Highly Sensitive N-(1-Naphthyl)Ethylene Diamine Method for the Spectrophotometric Determination of Trace Amounts of Nitrite in Various Water Samples

Nagaraja, Padmarajaiah; Shivaswamy, Mattighatta; Kumar, H.C. Charan

doi:10.1080/03067310108044384

Cited by 17 publications

(7 citation statements)

References 23 publications

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“…The bacterial cell growth was determined by spectrophotometry (Barman et al, ), the absorbance was measured at 600 nm with a visible spectrophotometer (OD 600 ).

{NO}_{2}^{-}

‐N concentration was determined using N‐(1‐naphthyl)‐ethylenediamine dihydrochloride spectrophotometric method (Nagaraja, Shivaswamy, & Kumar, ). NH 4 + ‐N concentration was determined with indophenol blue colorimetric spectrophotometric method (Pai, Tsau, & Yang, ).…”

Section: Methodsmentioning

confidence: 99%

Characterization of a novel marine origin aerobic nitrifying–denitrifying bacterium isolated from shrimp culture ponds

Zhao

Tian

et al. 2019

Aquac Res

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A novel marine origin Bacillus subtilis strain H1 isolated from a shrimp culture pond effectively removed NH4+‐N, NO2-‐N and NO3-‐N, with a maximum ammonium, nitrite and nitrate removal rate of 2.35 mg NH4+‐N L−1 hr−1 per OD, 9.64 mg NO2-‐N L−1 hr−1 per OD and 0.75 mg NO3-‐N L−1 hr−1 respectively. The gas chromatography–isotope ratio mass spectrometry results indicated that N2O was emitted when 15NH4Cl, Na15NO2 or Na15NO3 was used. Additionally, N2 was also produced when Na15NO2 was used. Single‐factor experiments suggested that the optimal conditions for NH4+‐N and NO2-‐N removal were glucose as a carbon source, C/N 15, initial pH 7.5, 30 g/L NaCl, 28°C and a shaking speed of 160 rpm. Orthogonal tests showed that the optimal conditions for NH4+‐N removal were C/N 15, pH 9, 10 g/L NaCl and shaking speed 160 rpm when ammonium chloride was used as the substrate. The optimal conditions for NO2-‐N removal were C/N 10, pH 6, 10 g/L NaCl and a shaking speed of 160 rpm when sodium nitrite was used as the substrate. In summary, B. subtilis strain H1 had highly efficient aerobic nitrifying–denitrifying ability and high adaptability, suggesting that it is potentially valuable to marine aquaculture.

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“…The bacterial cell growth was determined by spectrophotometry (Barman et al, ), the absorbance was measured at 600 nm with a visible spectrophotometer (OD 600 ).

{NO}_{2}^{-}

Section: Methodsmentioning

confidence: 99%

Characterization of a novel marine origin aerobic nitrifying–denitrifying bacterium isolated from shrimp culture ponds

Zhao

Tian

et al. 2019

Aquac Res

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“…Many methods have been reported for quantitative determination of nitrite, including kinetic (Koupparis et al 1982;Montes and Laserna 1987;Ensafi and Samimifar 1993;Xingguo et al 1996), chromatographic (Binghui et al 2006;Mou et al 1993), potentiometric (Li et al 1994;Schaaler et al 1994), amperometric (Bertotti and Pletcher 1997), flowinjection (Kojo and Gorodkiewicz 1995;Ahmed et al 1996), polorographic (Gao et al 1990), spectrophotometric methods (Nagaraja and Hemanthakumar 2001;Nagaraja and Hemanthakumar 2002) and Raman spectroscopic (Wada and Hattori 1971) methods. Although, these methods are quite sensitive, they are plagued by several limitations as they are tedious, time consuming and require fairly sophisticated equipment.…”

Section: Introductionmentioning

confidence: 99%

Sensitive spectrophotometric methods for the assessment of nitrite in water sample

Nagaraj

Prakash

Shivakumar

et al. 2008

Environ Monit Assess

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Two spectrophotometric methods have been developed for the determination of nitrite using dapsone (DAP) with alpha-naphthol and 4-amino-5-hydroxynapthalene-2,7-disulphonic acid monosodium salt (AHNDMS) as chromogenic reagents with maximum absorbance wavelength at 540 and 520 nm respectively. For the method that utilizes dapsone with alpha-naphthol (DAP-alpha-naphthol), the beer's law range is obeyed between 0.05-0.8 microg ml(-1) with molar absorptivity of 5.749 x 104 l mol(-1) cm(-1). The second method that uses dapsone with AHNDMS (DAP-AHNDMS), the beer's law is valid over the range 0.2-1.4 mug ml(-1) and molar absorptivity 2.44 x 104 l mol(-1) cm(-1). The common interfering ions in the analytical procedures have been studied. This proposed methods are reliable, reproducible and have been successfully applied to determine nitrite in various water sources of environmental interest.

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“…Among the spectrophotometric methods that are adopted as an AOAC (Association of Official Analytical Chemists) official method of analysis for nitrite determination is its reaction with N-(1-naphthyl)-ethylenediamine hydrochloride (NEDH) and sulfanilamide (SA) (AOAC Methods 1995). Several aromatic amines have been used for diazotization such as 4-aminobenzoic acid (Flamerz and Bashir 1981b), 4-aminophenylmercaptoasetic acid (Tarafder and Rathore 1988), Dapsone and iminodibenzyl (Nagaraja et al 2001a), N-(1-naphthyl)ethylene diamine (Nagaraja et al 2001b),4,4 0 -diaminostilbene-2,2 0 -disulphonic acid H. Ozmen et al 824 (Reddy 2004), sulfathiazole (Horita et al 1977;Helaleh and Korenaga 2001), p-nitroaniline (Kesari and Gupta 1998;Revanasiddappa et al 2001;Sreekumar et al 2003;Revanasiddappa et al 2003), and 4,4-methylenebis-m-nitroaniline (Suvardhan et al 2005). Active methylene compounds such as acetyl acetone (Revanasiddappa et al 2001), ethyl acetoacetate (Suvardhan et al 2005), or dibenzoylmethane (Shanthi and Balasubramanian 1994) have also been used in coupling.…”

Section: Introductionmentioning

confidence: 99%

Spectrophotometric Determination of Nitrite in Water Samples with 4‐(1‐Methyl‐1‐Mesitylcyclobutane‐3‐yl)‐2‐Aminothiazole

2006

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A simple, sensitive, and specific spectrophotometric method for the measurement of nitrite in water has been developed and optimum reaction conditions along with other analytical parameters have been evaluated. The azo dye, 4-(1-methyl-1-mesitylcylobutane-3-yl)-2-( p-N,N-dimethylazobenzene)-1,3-thiazole was synthesized with the reaction of 4-(1-methyl-1-mesitylcylobutane-3-yl)-2-aminothiazole and N,N-dimethyl aniline in acidic medium. Obtained azo dye has been characterized by infrared (IR), 1 H nuclear magnetic resonance (NMR), and microanalysis methods. The dye shows an absorption maximum at 482 nm. The method is optimized for acid concentration, pH, amount of reagents required, time, and interfering species. All the determinations were carried out at this wavelength throughout the work. At an analytical wavelength of 482 nm, Beer's law is obeyed over the concentration range 0.05 to 2.00 mg nitrite per mL analyte. The molar absorptivity, Sandell's sensitivity, and relative standard deviation are 2.03 Â 10 4 L mol 21 cm 21 + 251.3 (95%), 2.28 Â 10 23 mg cm 22 , and 2.74% (n ¼ 10), respectively. The detection limit of the method is 0.012 mg ml 21 of nitrite ion. The method was succesfully applied to the determination of nitrite in tap water and lake water.

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Highly Sensitive N-(1-Naphthyl)Ethylene Diamine Method for the Spectrophotometric Determination of Trace Amounts of Nitrite in Various Water Samples

Cited by 17 publications

References 23 publications

Characterization of a novel marine origin aerobic nitrifying–denitrifying bacterium isolated from shrimp culture ponds

Characterization of a novel marine origin aerobic nitrifying–denitrifying bacterium isolated from shrimp culture ponds

Sensitive spectrophotometric methods for the assessment of nitrite in water sample

Spectrophotometric Determination of Nitrite in Water Samples with 4‐(1‐Methyl‐1‐Mesitylcyclobutane‐3‐yl)‐2‐Aminothiazole

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