Highly sensitive phenolic biosensor based on magnetic polydopamine-laccase-Fe3O4 bionanocomposite

Specificity enhancement towards phenolic substrate by immobilization of laccase on surface plasmon resonance sensor chip

“…Therefore, detection and elimination of hazardous phenolic compounds using laccases has gained attention during recent decades. To date, various biosensors for both environmentally important pollutants and clinically relevant metabolites have been developed [5][6][7]. Although the determination of phenol and its derivative compounds is of environmental greatness [8,9], but in clinical detection system preparation, specific detection of a single substrate in complex mixtures is more critical and requires the use of enzymes with high substrate specificity, which means that laccase with broad range specificity [10], could not be a highly suitable candidate for detection.…”

Poly-dopamine-beta-cyclodextrin: A novel nanobiopolymer towards sensing of some amino acids at physiological pH

“…Recently, a novel Chronocoulometric DNA biosensor based on a GCE modified with gold nanoparticles, PDA and carbon nanotubes has been reported, lately [60]. Also, A phenolic biosensor based on magnetic PDA has been introduced, as well [61]. More importantly, PDA reduced graphene oxide nanocomposite has been applied for electrochemical application in simultaneous determination of hydroquinone and catechol [62].…”

Determination of Phenol and Chlorophenols at Single-Wall Carbon Nanotubes/Poly(3,4-ethylenedioxythiophene) Modified Glassy Carbon Electrode Using Flow Injection Amperometry

“…Tyrosinase (Tyr) based sensors have been the most widely used amperometric biosensor for determination of phenols and were prepared in a number of matrices and conventional electrodes, including carbon paste [13], conducting polymers [14,15], CNT conducting polymer composites [16,17], self-assembled monolayer [18], and silica sol-gel composite [19]. Laccase based biosensors [20,21] and horseradish peroxidase (HRP) [22] based electrochemical detectors coupled with flow injection system for the determination of phenol and phenol derivatives were also reported. In the investigated biosensors, the enzymes were either individually immobilized or composite multienzyme systems were immobilized on conventional electrodes to determine the phenolic compounds.…”