Highly sensitive polysilicon wire sensor for DNA detection using silica nanoparticles/γ-APTES nanocomposite for surface modification

Wu, You‐Lin; Lin, Jing‐Jenn; Hsu, Po Kuei; Hsu, Chung-Ping

doi:10.1016/j.snb.2011.01.035

Cited by 33 publications

(19 citation statements)

References 29 publications

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“…To overcome and solve such drawbacks, technological solutions have been envisioned and tested like the, immobilization of specific probes and/or biomolecules onto the gate-dielectric surface. This alternative approach was successfully developed for the fabrication of bio-FETs (biologically modified field-effect transistors) with the use and grafting of biomolecules (Uslu, 2004;Wu, 2011;Tatikonda, 2013;Hammock, 2013;Lee, 2009b), leading to sensors with higher specificity and selectivity.…”

Section: Introductionmentioning

confidence: 99%

A field effect transistor biosensor with a γ-pyrone derivative engineered lipid-sensing layer for ultrasensitive Fe3+ ion detection with low pH interference

Nguyen

Labed

Zein

et al. 2014

Biosensors and Bioelectronics

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Field effect transistors have risen as one of the most promising techniques in the development of biomedical diagnosis and monitoring. In such devices, the sensitivity and specificity of the sensor rely on the properties of the active sensing layer (gate dielectric and probe layer). We propose here a new type of transistor developed for the detection of Fe(3+) ions in which this sensing layer is made of a monolayer of lipids, engineered in such a way that it is not sensitive to pH in the acidic range, therefore making the device perfectly suitable for biomedical diagnosis. Probes are γ-pyrone derivatives that have been grafted to the lipid headgroups. Affinity constants derived for the chelator/Fe(3+) complexation as well as for other ions demonstrate very high sensitivity and specificity towards ferric ions with values as high as 5.10(10) M and a detected concentration as low as 50 fM.

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Section: Introductionmentioning

confidence: 99%

A field effect transistor biosensor with a γ-pyrone derivative engineered lipid-sensing layer for ultrasensitive Fe3+ ion detection with low pH interference

Nguyen

Labed

Zein

et al. 2014

Biosensors and Bioelectronics

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“…Polysilicon was chosen for its ability to withstand high temperatures and its high compatibility with standard semiconductor processing steps. The advantages of this material also include high sensitivity, a fast response time and high accuracy, as previously reported [ 34 , 35 ]. In the quest to reduce the use of expensive equipment, a conventional lithography microfabrication technique was implemented; this technique can be used to fabricate simple and non-complex nanostructures such as nanogaps and nanowires [ 36 , 37 ].…”

Section: Introductionmentioning

confidence: 62%

A Point-of-Care Immunosensor for Human Chorionic Gonadotropin in Clinical Urine Samples Using a Cuneated Polysilicon Nanogap Lab-on-Chip

et al. 2015

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Human chorionic gonadotropin (hCG), a glycoprotein hormone secreted from the placenta, is a key molecule that indicates pregnancy. Here, we have designed a cost-effective, label-free, in situ point-of-care (POC) immunosensor to estimate hCG using a cuneated 25 nm polysilicon nanogap electrode. A tiny chip with the dimensions of 20.5 × 12.5 mm was fabricated using conventional lithography and size expansion techniques. Furthermore, the sensing surface was functionalized by (3-aminopropyl)triethoxysilane and quantitatively measured the variations in hCG levels from clinically obtained human urine samples. The dielectric properties of the present sensor are shown with a capacitance above 40 nF for samples from pregnant women; it was lower with samples from non-pregnant women. Furthermore, it has been proven that our sensor has a wide linear range of detection, as a sensitivity of 835.88 μA mIU-1 ml-2 cm-2 was attained, and the detection limit was 0.28 mIU/ml (27.78 pg/ml). The dissociation constant Kd of the specific antigen binding to the anti-hCG was calculated as 2.23 ± 0.66 mIU, and the maximum number of binding sites per antigen was Bmax = 22.54 ± 1.46 mIU. The sensing system shown here, with a narrow nanogap, is suitable for high-throughput POC diagnosis, and a single injection can obtain triplicate data or parallel analyses of different targets.

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“…However, to fabricate microfluidic chips, the open microchannels need to be sealed. A number of different bonding techniques such as APTES bonding [9,10], thermal bonding [11][12][13], solvent bonding [14,15] and microwave welding methods [16] have been reported in recent several years. Among these bonding methods, thermal bonding has attracted increasing interests due to its simple and low-cost characters.…”

Section: Introductionmentioning

confidence: 99%

A fast and simple bonding method for low cost microfluidic chip fabrication

Yin

Zou

2018

Journal of Electrical Engineering

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With the development of the microstructure fabrication technique, microfluidic chips are widely used in biological and medical researchers. Future advances in their commercial applications depend on the mass bonding of microfluidic chip. In this study we are presenting a simple, low cost and fast way of bonding microfluidic chips at room temperature. The influence of the bonding pressure on the deformation of the microchannel and adhesive tape was analyzed by numerical simulation. By this method, the microfluidic chip can be fully sealed at low temperature and pressure without using any equipment. The dye water and gas leakage test indicated that the microfluidic chip can be bonded without leakage or block and its bonding strength can up to 0.84 MPa.

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Highly sensitive polysilicon wire sensor for DNA detection using silica nanoparticles/γ-APTES nanocomposite for surface modification

Cited by 33 publications

References 29 publications

A field effect transistor biosensor with a γ-pyrone derivative engineered lipid-sensing layer for ultrasensitive Fe3+ ion detection with low pH interference

A field effect transistor biosensor with a γ-pyrone derivative engineered lipid-sensing layer for ultrasensitive Fe3+ ion detection with low pH interference

A Point-of-Care Immunosensor for Human Chorionic Gonadotropin in Clinical Urine Samples Using a Cuneated Polysilicon Nanogap Lab-on-Chip

A fast and simple bonding method for low cost microfluidic chip fabrication

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