Highly sensitive spectrofluorimetric method for rapid determination of orciprenaline in biological fluids and pharmaceuticals

Abstract

Orciprenaline sulphate (ORP) is a direct‐acting sympathomimetic with mainly beta‐adrenoceptor stimulant activity. It is used as a bronchodilator in the management of reversible airway obstruction. For the first time, a rapid highly sensitive spectrofluorimetric method is described that is relied on measuring the fluorescence spectra of ORP at acidic pH and without addition of any chemical reagents. The relative fluorescence intensity was measured at 310 nm and after excitation at 224 nm. ORP native fluorescence was calibrated in both water and acetonitrile as diluting solvents. The method was designed to estimate the drug in miscellaneous matrices with high accuracy and precision. Linear ranges of calibration curves were 30.0–400.0 ng/ml and 10.0–240.0 ng/ml in water and acetonitrile, respectively. The detection limits were calculated and reached as low as 3.3 and 3.1 ng/ml, respectively, representing the ultra‐sensitivity of the proposed method. This result permitted application of this method for spiked human plasma and urine and was used as a preliminary investigation with good percentage recovery (89.4–106.8%). The application was further extended to analyse ORP in its pharmaceutical formulations. The method was validated in compliance with International Council of Harmonization (ICH) Guidelines.

Economic Spectrofluorometric Bioanalysis of Empagliflozin in Rats’ Plasma

“…Its capacity of detection is approximately one order of magnitude greater than that of molecular absorption spectroscopy, and its selectivity is clearly greater than that of other spectroscopic methods. Accordingly, spectrofluorometry has been applied for the analysis of different analytes in complex matrices [ 29 – 31 ]. Furthermore, in the developing countries (that include Egypt), the application of spectrofluorometry in bioanalysis continues to be popular due to the simplicity, the common availability of the instrumentation, and the low cost without the need of costly infrastructure that is not applicable in QC labs with limited fund.…”

Solid-phase extraction and validated spectrofluorimetric quantification of pamidronate in human plasma

“…Spectrofluorimetric methods are advantageous in many aspects. They are highly sensitive [9]. In addition, spectrofluorimetric methods offer a high degree of selectivity because not all substances that absorb in the UV-region emit fluorescence.…”