Highly sensitive systems for experimental insertional mutagenesis in repair-deficient genetic environment in Drosophila melanogaster: New opportunities for studying postreplication repair of double-stranded DNA breaks and mechanisms of transposable element migration

Chmuzh, E. V.; Shestakova, L. A.; Volkova, Valeriya; Захаров, И. К.

doi:10.1134/s1022795407010073

Cited by 2 publications

(2 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…mus210 G1 (genotype: mus210 G1 /CyO) are strain of the E-cytotype with a mutation in the mus210 gene, which participates in excision repair (Sekelsky et al, 1998). The presence of mutations in genes responsible for the excision repair in the genome does not impair the stability of chromosomes experiencing transposon activity (Chmuzh et al, 2007). We used this strain as a conditional negative control.…”

Section: Strains Without Hobo Transposonsmentioning

confidence: 99%

Involvement of DNA Repair Genes and System of Radiation-Induced Activation of Transposons in Formation of Transgenerational Effects

Yushkova

2020

Front. Genet.

View full text Add to dashboard Cite

The study of the genetic basis of the manifestation of radiation-induced effects and their transgenerational inheritance makes it possible to identify the mechanisms of adaptation and possible effective strategies for the survival of organisms in response to chronic radioactive stress. One persistent hypothesis is that the activation of certain genes involved in cellular defense is a specific response of the cell to irradiation. There is also data indicating the important role of transposable elements in the formation of radiosensitivity/radioresistance of biological systems. In this work, we studied the interaction of the systems of hobo transposon activity and DNA repair in the cell under conditions of chronic low-dose irradiation and its participation in the inheritance of radiation-induced transgenerational instability in Drosophila. Our results showed a significant increase of sterility and locus-specific mutability, a decrease of survival, fertility and genome stability (an increase the frequency of dominant lethal mutations and DNA damage) in non-irradiated F1/F2 offspring of irradiated parents with dysfunction of the mus304 gene which is responsible for excision and post-replicative recombination repair and repair of double-stranded DNA breaks. The combined action of dysfunction of the mus309 gene and transpositional activity of hobo elements also led to the transgenerational effects of irradiation but only in the F1 offspring. Dysfunction of the genes of other DNA repair systems (mus101 and mus210) showed no visible effects inherited from irradiated parents subjected to hobo transpositions. The mei-41 gene showed specificity in this type of interaction, which consists in its higher efficiency in sensing events induced by transpositional activity rather than irradiation.

show abstract

Section: Strains Without Hobo Transposonsmentioning

confidence: 99%

Involvement of DNA Repair Genes and System of Radiation-Induced Activation of Transposons in Formation of Transgenerational Effects

Yushkova

2020

Front. Genet.

View full text Add to dashboard Cite

show abstract

“…is defected in the gene mus304 (allele D1) which takes in post-replication DNA repair and recombination, in the repair DSB and excision repair of pyrimidine dimers (Harris and Boyd 1993;Brodsky et al 2000;Chmuzh et al 2007), homolog of mammal ATRIP gene (Oikemus et al 2006 (Chan et al 2010;D ıaz-Vald es et al 2010), homolog of mammal and human Polq gene (Sharief et al 1999;Shima et al 2004); stock mus309 (genotype: mus309[D3,ry]/CyO) (allele D3) has violation of the system repair DSB (Beall and Rio 1997;Portin 2010), homolog of mammal Kup70 and human XRCC6 genes (Beall and Rio 1996;Pei et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Interaction between gene repair and mobile elements-induced activity systems after low-dose irradiation

Yushkova¹,

Zainullin²

2016

International Journal of Radiation Biology

View full text Add to dashboard Cite

Purpose: To analyze the role of mus-genes repair in system activation P-elements in Drosophila melanogaster induced by a chronic exposure to low doses. Materials and methods: The materials were dysgenic individuals of Drosophila melanogaster with mutations in repair genes (mus101, mus205, mus304, mus308, mus309) and simultaneous transposition of mobile P-elements. The animals were exposed to a chronic irradiation in low doses (0.42 mGy/h). The reaction of animals was analyzed by the DNA damage rate in somatic cells ('Comet assay'), level of dominant lethal mutations, fecundity, and survival rate. Results: The combined action of the systems of post-replication, recombination repair (mus205, mus304), repair of DNA double-stranded breaks (mus304), and of the transposition activity of P-elements after a chronic irradiation in low doses was identified according to every study parameter. The other repair systems and their genes (mus101, mus308, and mus309) responded to action of only one factor (irradiation or mobile elements transposition). Conclusion: The obtained data significantly contribute to the knowledge on a new reaction of the mechanisms of organisms to a chronic irradiation in low doses. ARTICLE HISTORY

show abstract

Highly sensitive systems for experimental insertional mutagenesis in repair-deficient genetic environment in Drosophila melanogaster: New opportunities for studying postreplication repair of double-stranded DNA breaks and mechanisms of transposable element migration

Cited by 2 publications

References 20 publications

Involvement of DNA Repair Genes and System of Radiation-Induced Activation of Transposons in Formation of Transgenerational Effects

Involvement of DNA Repair Genes and System of Radiation-Induced Activation of Transposons in Formation of Transgenerational Effects

Interaction between gene repair and mobile elements-induced activity systems after low-dose irradiation

Contact Info

Product

Resources

About