HILIC quantification of Oenotheralanosterol A and B from Oenothera biennis and their suppression of IL-6 and TNF-α expression in mouse macrophages

Antiproliferative and antimicrobial efficacy of the compounds isolated from the roots of Oenothera biennis L.

“…In an attempt to investigate novel activity of compounds/mixture isolated from the roots of O. biennis L., herein we are reporting antiproliferative and antimicrobial potential of seven compounds and one mixture (1–8), among which two were isolated and reported for the first time by our group …”

“…The roots of this plant are edible as vegetables and thus may have a role in health and disease. In prior findings, our group reported the isolation and structure elucidation of the compounds/mixture (1–8) from the roots of O. biennis L., and their antioxidant and anti‐inflammatory activity . Hence, to acquiesce our earlier findings and give a scientific explanation to its dietary and traditional usage, the present work was undertaken to examine the antiproliferative efficacy of the isolated compounds/mixture (1–8) from the roots of O. biennis L. Besides cytotoxicity analysis, a probable mechanism for antiproliferative activity has also been worked out utilizing in‐silico and in‐vitro experiments.…”

“…Seven compounds and one mixture (1)(2)(3)(4)(5)(6)(7)(8) were isolated and characterized from the roots of O. biennis L. in methanolic extracts and separated on silica gel and ODS (octadecyl silica) columns using different solvents for the elution with a gradient of n-hexane, n-hexane-ethyl acetate (9 : 1-1 : 9, v/v), ethyl acetate and ethyl acetate-methanol (9 : 1-1 : 9, v/v) for silica gel column and water-methanol for ODS column. [12,13,15] After isolation and characterization of all the compounds, it was found that 4 was a mixture of 1 and 3. Figure 1 represents the chemical structures and stereochemistry of all the compounds/mixture isolated from the roots of O. biennis L.…”

“…In prior findings, our group reported the isolation and structure elucidation of the compounds/ mixture (1)(2)(3)(4)(5)(6)(7)(8) from the roots of O. biennis L., and their antioxidant and anti-inflammatory activity. [13][14][15][16] Hence, to acquiesce our earlier findings and give a scientific explanation to its dietary and traditional usage, the present work was undertaken to examine the antiproliferative efficacy of the isolated compounds/mixture (1)(2)(3)(4)(5)(6)(7)(8) from the roots of O. biennis L. Besides cytotoxicity analysis, a probable mechanism for antiproliferative activity has also been worked out utilizing in-silico and in-vitro experiments. Additionally, antimicrobial efficacy of these compounds has also been explored both qualitatively and quantitatively using agar diffusion and broth dilution assays.…”

In Silico Assay Development for Screening of Tetracyclic Triterpenoids as Anticancer Agents against Human Breast Cancer Cell Line MCF7

“…Triterpenoids are known for their cytotoxic and anti-inflammatory activity from plant sources [3], [4]. Terpenoids are divided into many groups on the basis of their structures.…”