α-(-)-bisabolol is a natural monocyclic sesquiterpene present in the essential oil has generated considerable interest in the chemical and pharmaceutical industries and currently in use in various formulations, mainly in cosmetics. This study was undertaken to evaluate its therapeutic profile against skin inflammation using in-vitro, in-vivo and in-silico assays. Lipopolysachharide (LPS) and 12-O-tetradecanoyl-phorbol-13-acetate (TPA)-induced production of proinflammatory cytokines (TNF-α and IL-6) in macrophage cells as well as in TPA-induced skin inflammation in mice was significantly inhibited by α-(-)-bisabolol. TPA-induced ear thickness, ear weight and lipid peroxidation and histopathological damage in the ear tissue were also significantly inhibited by topical application of α-(-)-bisabolol in a dose dependent manner. In-vitro and in-vivo toxicity profiles indicate that it is safe for topical application on skin. Molecular docking study also revealed its strong binding affinity to the active site of the pro-inflammatory proteins. These findings suggested that α-(-)-bisabolol may be a useful therapeutic candidate for the treatment of skin inflammation.

A precise method has been developed for measuring the activity of Ornithine decarboxylase (ODC) enzyme, a promising target of cancer and infectious diseases using petite quantity of chemicals. The modified spectrophotometric assay has been used to find inhibitors of ODC enzyme. The methodology is based on the cognizance that enzyme transforms L-ornithine hydrochloride (substrate) to yellow colored putrescine (product) soluble in pentanol, the absorbance of which was measured spectrophotometrically. The technique described herein was optimized for evaluating efficacy of phytomolecules as ODC attenuator but could also be used for exploration of novel bioactive compounds as chemopreventive and/or chemotherapeutic agents. A reaction concoction was prepared and hydrophobic withdrawal of yellow colored TNP-Putrescine-TNP complex was recorded at 426nm. Advanced validation and understanding of the factors affecting the enzyme activity i.e. substrate concentration, inhibitor concentration, temperature, pH of the reaction medium and incubation time were also investigated.

In this review, PubMed, Sci-finder and other search engines were used to gather information on Cathepsin D. The necessary and relevant information was thoroughly studied to make the article appropriate to highlight all the aspects related to Cathepsin D and its role in cancer. Findings & Conclusion: The present review illustrates structural, functional and regulatory aspects of CATD in cancer, its significant role in angiogenesis, metastasis, invasion, apoptosis, cell proliferation, and therapeutic potential besides the benefits of targeting CATD by the natural products in cancer chemoprevention.

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