Hippocampus and Prefrontal Cortex Modulation of Contextual Fear Memory Is Dissociated by Inhibiting De Novo Transcription During Late Consolidation

Pereira, Luciana Melo; Castro, Caio M. de; Guerra, Lorena Terene Lopes; Queiroz, Thaís M; Marques, João Trindade; Pereira, Grace Schenatto

doi:10.1007/s12035-018-1463-4

Cited by 13 publications

(13 citation statements)

References 73 publications

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“…Similar to findings in the hippocampus, de novo transcription as well as de novo protein synthesis are required in the mPFC at CFC encoding for proper memory consolidation [10,12,65]. More precisely, whole-ACC RNA-seq revealed a global shift in the mPFC transcriptome towards biological processes linked with synaptic plasticity, and a decreased expression of genes involved in immunity 1 h after conditioning [9].…”

Section: Post-learning Molecular Modifications In the Mpfcsupporting

confidence: 60%

The Medial Prefrontal Cortex and Fear Memory: Dynamics, Connectivity, and Engrams

Dixsaut

Gräff

2021

IJMS

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It is becoming increasingly apparent that long-term memory formation relies on a distributed network of brain areas. While the hippocampus has been at the center of attention for decades, it is now clear that other regions, in particular the medial prefrontal cortex (mPFC), are taking an active part as well. Recent evidence suggests that the mPFC—traditionally implicated in the long-term storage of memories—is already critical for the early phases of memory formation such as encoding. In this review, we summarize these findings, relate them to the functional importance of the mPFC connectivity, and discuss the role of the mPFC during memory consolidation with respect to the different theories of memory storage. Owing to its high functional connectivity to other brain areas subserving memory formation and storage, the mPFC emerges as a central hub across the lifetime of a memory, although much still remains to be discovered.

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Section: Post-learning Molecular Modifications In the Mpfcsupporting

confidence: 60%

The Medial Prefrontal Cortex and Fear Memory: Dynamics, Connectivity, and Engrams

Dixsaut

Gräff

2021

IJMS

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“…Critically, CFM consolidation can be disrupted by SD over the first 5 to 6 h following CFC ( 17 – 21 ). Over this same post-CFC interval, disruption of either neuronal activity ( 16 ), transcription ( 22 , 23 ), or translation ( 24 – 27 ) in the dorsal hippocampus can likewise disrupt consolidation. This suggests that an activity- and sleep-dependent mechanism, impinging on biosynthetic pathways in the hippocampus, is essential for consolidation.…”

mentioning

confidence: 99%

Hippocampal neurons’ cytosolic and membrane-bound ribosomal transcript profiles are differentially regulated by learning and subsequent sleep

Delorme

Wang

Kodoth

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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The hippocampus is essential for consolidating transient experiences into long-lasting memories. Memory consolidation is facilitated by postlearning sleep, although the underlying cellular mechanisms are largely unknown. We took an unbiased approach to this question by using a mouse model of hippocampally mediated, sleep-dependent memory consolidation (contextual fear memory). Because synaptic plasticity is associated with changes to both neuronal cell membranes (e.g., receptors) and cytosol (e.g., cytoskeletal elements), we characterized how these cell compartments are affected by learning and subsequent sleep or sleep deprivation (SD). Translating ribosome affinity purification was used to profile ribosome-associated RNAs in different subcellular compartments (cytosol and membrane) and in different cell populations (whole hippocampus, Camk2a+ neurons, or highly active neurons with phosphorylated ribosomal subunit S6 [pS6+]). We examined how transcript profiles change as a function of sleep versus SD and prior learning (contextual fear conditioning; CFC). While sleep loss altered many cytosolic ribosomal transcripts, CFC altered almost none, and CFC-driven changes were occluded by subsequent SD. In striking contrast, SD altered few transcripts on membrane-bound (MB) ribosomes, while learning altered many more (including long non-coding RNAs [lncRNAs]). The cellular pathways most affected by CFC were involved in structural remodeling. Comparisons of post-CFC MB transcript profiles between sleeping and SD mice implicated changes in cellular metabolism in Camk2a+ neurons and protein synthesis in highly active pS6+ (putative “engram”) neurons as biological processes disrupted by SD. These findings provide insights into how learning affects hippocampal neurons and suggest that the effects of SD on memory consolidation are cell type and subcellular compartment specific.

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“…It is important to highlight that the SSEP provides a unique perspective on evaluating circuitry involved in sensory signal processing once its “tags” the electrophysiological recordings of such brain substrates with a distinct spectral signature assigned by the modulation frequency. Moreover, since the footshock can be precisely manipulated regarding its parameters the system can be used in different behavioral models, as an example of contextual fear conditioning (Pereira et al, 2019) or in studies of post-traumatic stress disorder (Bali and Jaggi, 2015).…”

Section: Discussionmentioning

confidence: 99%

A Custom Microcontrolled and Wireless-Operated Chamber for Auditory Fear Conditioning

et al. 2019

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Animal behavioral paradigms, such as classical conditioning and operant conditioning, are an important tool to study the neural basis of cognition and behavior. These paradigms involve manipulating sensory stimuli in a way that learning processes are induced under controlled experimental conditions. However, the majority of the commercially available equipment did not offer flexibility to manipulate stimuli. Therefore, the development of most versatile devices would allow the study of more complex cognitive functions. The purpose of this work is to present a low-cost, customized and wireless-operated chamber for animal behavior conditioning, based on the joint operation of two microcontroller modules: Arduino Due and ESP8266-12E. Our results showed that the auditory stimulation system allows setting the carrier frequency in the range of 1 Hz up to more than 100 kHz and the sound stimulus can be modulated in amplitude, also over a wide range of frequencies. Likewise, foot-shock could be precisely manipulated regarding its amplitude (from ∼200 μA to ∼1500 μA) and frequency (up to 20 pulses per second). Finally, adult rats exposed to a protocol of cued fear conditioning in our device showed consistent behavioral response and electrophysiological evoked responses in the midbrain auditory pathway. Furthermore, the device developed in the current study represents an open source alternative to develop customized protocols to study fear memory under conditions of varied sensory stimuli.

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Hippocampus and Prefrontal Cortex Modulation of Contextual Fear Memory Is Dissociated by Inhibiting De Novo Transcription During Late Consolidation

Cited by 13 publications

References 73 publications

The Medial Prefrontal Cortex and Fear Memory: Dynamics, Connectivity, and Engrams

The Medial Prefrontal Cortex and Fear Memory: Dynamics, Connectivity, and Engrams

Hippocampal neurons’ cytosolic and membrane-bound ribosomal transcript profiles are differentially regulated by learning and subsequent sleep

A Custom Microcontrolled and Wireless-Operated Chamber for Auditory Fear Conditioning

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