Hirsutella thompsonii as a mycoacaricide for Aceria guerreronis on coconut in India: research, development, and other aspects

Kumar, Prabhat

doi:10.1007/978-90-481-9837-5_73

Cited by 6 publications

(5 citation statements)

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“…Hirsutella thompsonii Fisher (Ophiocordycipitaceae, Hypocreales, Sordariomycetes, Ascomycota) was first described on citrus rust mites (Phyllocoptruta oleivora) in Florida (Fisher, 1950). Since then, it has been reported to be pathogenic to various mite pests in diverse ecosystems (Weibelzahl and Liburd, 2009;Kumar, 2009;Ferreira Picoli and Vieira, 2013;Nuvoloni et al, 2014). As important phytophagous pests of agricultural crops worldwide, mites can cause serious damage and yield losses when reaching the outbreak level (Singh and Raghuraman, 2011;Rezende et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…As important phytophagous pests of agricultural crops worldwide, mites can cause serious damage and yield losses when reaching the outbreak level (Singh and Raghuraman, ; Rezende et al ., ). As long‐term reliance of mite control on chemical acaricides has caused resistance and public concerns of residues in agroproducts, exploiting biopesticides has been suggested as sustainable alternatives to conventional pesticides (Kumar, ). H. thompsonii is reported to produce phomalactone (Krasnoff and Gupta, ) as well as the protein hirsutellin (Herrero‐Galán et al ., ; ), which are toxic to a variety of agricultural pests.…”

Section: Introductionmentioning

confidence: 99%

“…The fungus is safe to human, mammals, predators and parasites, and has been one of the few fungal species that were commercially exploited as bioinsecticides. For example, spraying of Mycohit, a powder formulation of the fungus, on young bunches resulted in high fungus‐associated mortality of the coconut mite Aceria guerreronis in many locations (Kumar, ). ALMITE, a liquid formulation of the fungus, was claimed to control red, pink and yellow mites in tea, rose, fruit, vegetables and plantation crops by foliar application (http://www.iplbiologicals.com/hirsutella-thompsonii/#!).…”

Section: Introductionmentioning

confidence: 99%

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Mitochondrial genome, comparative analysis and evolutionary insights into the entomopathogenic fungus Hirsutella thompsonii

Wang

Zhang

et al. 2018

Environmental Microbiology

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Nuclear genomes of two isolates of Hirsutella thompsonii, a pathogen causing epizootics among mites, have been reported; in contrast, its mitochondrial genome (mitogenome) has remained unknown, limiting our understanding of its evolution. Herein, we annotated the first complete mitogenome of H. thompsonii, which encoded all standard fungal mitochondrial genes plus three free-standing ORFs. Transcriptional analyses validated the expression of most conserved genes and revealed some interesting transcription patterns of mitochondrial genes. Phylogenetic analyses confirmed its placement in Ophiocordycipitaceae. Comparison of five different isolates originally collected from different locations revealed mitogenome size variations (60.3-66.4 kb) mainly due to different numbers of introns. A total of 15 intron loci were identified, with 11 existing in all 5 isolates and 4 showing presence/absence dynamics. These introns were most likely obtained through horizontal transfer from other fungal organisms. Those common introns might have been in H. thompsonii mitogenomes since the divergence of the fungus from its putative sister species H. minnesotensis, whereas those dynamic introns might have experienced 1-2 gain or loss events. We also detected evidence of degeneration for some introns. Overall, our study shed new insights into the mitochondrial evolution of the acaropathogenic fungus H. thompsonii.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mitochondrial genome, comparative analysis and evolutionary insights into the entomopathogenic fungus Hirsutella thompsonii

Wang

Zhang

et al. 2018

Environmental Microbiology

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“…Later, two liquid variants of this product, viz. Mycohit-LG20 and Mycohit-OS, have also been formulated, extensively field-tested and found to be equally effective (Sreerama Kumar 2006). Unlike most of the commercial mycopesticides, which contain only spores or conidia (Jenkins et al 1998), these bioproducts include both mycelia and conidia: mycelia for secondary cycling and disseminating the fungus, and conidia to target stray mites and to passively enter the perianth along with the spray fluid.…”

Section: Introductionmentioning

confidence: 98%

Enabling mycelial application of Hirsutella thompsonii for managing the coconut mite

Kumar

Singh

2008

Exp Appl Acarol

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Laboratory and field studies were conducted to examine the prospect of mycelial application of Hirsutella thompsonii as an alternative to the use of mycelial-conidial formulations of the fungus in the suppression of the coconut mite, Aceria guerreronis. In a series of laboratory experiments, glycerol, yeast extract powder and dehydrated malt extract broth were found to be the best among nine substances investigated as possible adjuvants for use on coconut palms in the field along with H. thompsonii mycelia. H. thompsonii biomass in the presence of adjuvants not only produced more colonies but also yielded more conidia per pellet. In terms of the density of conidia generated on a mycelial mat the treatments varied highly significantly in two methods, with glycerol showing an average of 106% increase over control. Though irradiance with simulated sunlight resulted in reduced conidiogenesis, in general, adjuvant-treated pellets, both exposed and unexposed to simulated sunlight, produced substantial conidiation compared with control, irrespective of the two incubation conditions. Better conidiation was observed under alternating light-dark regime than under total darkness in all the treatments. Glycerol boosted the pathogenicity of H. thompsonii by 16.5% over control. In the field, a newly developed mycelial formulation of H. thompsonii applied after tank-mixing separately with the three selected adjuvants brought down the post-treatment population of the coconut mite by 85.6-97.1%. Application of the fungus in combination with glycerol resulted in a tolerable mean nut damage grade of 2.0 during the pre-harvest stage, compared with an acute score of 4.0 in control palms.

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“…Entomopathogenic fungi such as Beauveria bassiana, Metarhizium anisopliae, Lecanicillium lecanii, Nomuraea rileyi, Hirsutella thompsonii, etc. showed excellent insecticidal activity [12][13][14][15][16]. For instance, Isaria tenuipes against the dengue vector Aedes aegypti (Linn.)…”

Section: Introductionmentioning

confidence: 99%

Insecticidal characteristics and structural identification of the potential active compounds from Streptomyces sp. KR0006: Strain improvement through mutagenesis

Kim

Umurzokov

Cho³

et al. 2022

PLoS ONE

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Pest control by biological means is an effective, eco-friendly, and promising method that typically involves compounds naturally derived from actinomycetes. Thus, the present study aimed to screen, characterize, and identify the structure of insecticidal compounds from Streptomyces sp. KR0006 and increase the activity through mutagenesis. In the examination of the insecticidal activity level of the isolates, Streptomyces sp. KR0006 metabolite showed significant activity against larvae and moths of Plutella xylostella. Taxonomic analyses of the 16S rRNA gene sequences revealed that the isolated KR0006 strain tended to be 99% consistent with Streptomyces cinereoruber strain NBRC 12756. Three active compounds isolated from the culture filtrate of KR0006 were purified by solvent partition, mid-pressure liquid chromatography (MPLC), Sephadex LH20 column chromatography, and high-performance liquid chromatography (HPLC). By performing 1H-NMR, 13C-NMR, and 2D-NMR experiments, and high-resolution electrospray ionization mass spectrometry analysis, the 316-HP2, 316-HP3, and 316-HP5 compounds were inferred as antimycin A3a (MW, 519.; C26H36N2O9), antimycin A8a (MW, 534; C27H38N2O9), and antimycin A1a (MW, 548; C28H40N2O9) respectively. Mutant U67 obtained from exposure to ultraviolet (UV) irradiation (254 nm, height 17 cm) for 70 seconds resulted in a 70% more larval mortality than that of the initial wild culture. The second mutation of the culture broth enhanced insecticidal activity by 80 and 100% compared with the first mutation and initial medium, respectively. Our study found that Streptomyces sp. KR0006 strain produces insecticidal active compounds and could be used for practical pest management.

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Hirsutella thompsonii as a mycoacaricide for Aceria guerreronis on coconut in India: research, development, and other aspects

Cited by 6 publications

References 1 publication

Mitochondrial genome, comparative analysis and evolutionary insights into the entomopathogenic fungus Hirsutella thompsonii

Mitochondrial genome, comparative analysis and evolutionary insights into the entomopathogenic fungus Hirsutella thompsonii

Enabling mycelial application of Hirsutella thompsonii for managing the coconut mite

Insecticidal characteristics and structural identification of the potential active compounds from Streptomyces sp. KR0006: Strain improvement through mutagenesis

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