Histamine metabolism of the guinea‐pig gastric mucosa.

Abstract

1. The mobilization of gastric mucosal histamine as reflected by changes in formation and content has been studied in guinea‐pigs on feeding and after injections of pentagastrin or 2‐deoxy‐D‐glucose. 2. Re‐feeding fasting guinea‐pigs as well as injections of pentagastrin or 2‐deoxy‐D‐glucose raised the rate of mucosal histamine formation; pentagastrin induced a fourfold rise. 3. Some properties of the enzyme catalysing the formation of histamine were examined. The results indicated that this enzyme is histidine decarboxylase, L‐histidine carboxy‐lyase, E.C. 4.1.1. 22. 4. The enzyme imidazole‐N‐methyl transferase, E.C. 2.1.1.8, which carries out methylation of the imidazole ring to yield 1‐methyl‐4‐(beta‐aminoethyl)imidazole (methylhistamine), was found in high amounts in the mucosa. The enzyme did not change upon stimulation of the mucosa. 5. The metabolism of histamine within the gastric mucosa is discussed in relationship to a suggested role of the amine in exciting acid secretion.

A modified schayer procedure for the estimation of histidine decarboxylase activity: Its application on tissue extracts from gastric mucosa of various mammals

“…No! Human KAHLSON et al [ 11] HAgANSON et al [22] subject LINDELL and WESTLING [ 14] LORENZ et al [2] NOLL and LEVINE [ 151 Dog Rabbit Guinea-pig DENCKER et al [ 16] KAHLSON et al [11] LORENZ et al [2] WERLE and LORENZ [ 17] LORENZ et aL [2} KAHLSON et al [11] LORENZ et al [18] LORENZ et aL [2] BERGMARK et al [8] AURES et al [20] KIM and GLICK [21] AURES et al [3] H~,KANSON and OWMAN [ 19] AuREs et al [3] LORENZ et al [7] AuREs et al [3] ance. Again by analogy it is rather difficult to conceive that histamine has a physiological function in the secretory processes of the gastric mucosa if it is not intrinsically formed in most species, and not only in a few rodents which possess histamine-containing cells in the gastric mucosa that are not to be found in all other mammals [3].…”