Histamine operates Cl–-gated channels in crayfish neurosecretory cells

Abstract

SUMMARY We describe a histamine-activated Cl– conductance in the X-organ neurons from crayfish Cherax quadricarinatus, which has comparable properties to the homomultimeric histamine-gated ion channels described in Drosophila. Topical application of histamine inhibited spontaneous neuronal firing in the X-organ sinus gland tract, concomitant with an increase in the membrane conductance. In X-organ neurons in culture and under voltage-clamp conditions, histamine evoked outward currents at –40 mV that reversed at the Cl– equilibrium potential. Histamine sensitivity in these neurons had a half-maximal response(EC50)=3.3±1 μmol l–1, with a Hill number of 2.6±0.4. The histamine-evoked current was blocked by tiotidine, cimetidine, ranitidine and 256±11 and 483±11 μmol l–1, respectively) and d-tubocurarine(IC50=21±2 μmol l–1), but was insensitive to picrotoxin, bicuculline and strychnine. Neither GABA nor glutamate was capable of desensitizing the histamine response, indicating that histamine activates a particular Cl– conductance. The presence of immunoreactive neurons to histamine in the medulla terminalis with axonal projections to the neuropile suggests a possible histaminergic modulation of the X-organ sinus gland system.

Coordination of distinct but interacting rhythmic motor programs by a modulatory projection neuron using different co-transmitters in different ganglia

“…The recorded response to histamine's effect also served as the indicator for when the histamine receptor antagonists had fully blocked histamine responses. Although most histamine responses that have been examined in crustaceans are blocked by the H 2 receptor antagonist cimetidine (Bayer et al, 1989;Callaway and Stuart, 1989;El Manira and Clarac, 1994;Christie et al, 2004;Cebada and García, 2007;McCoole et al, 2011), it failed to block the responses to puffed histamine in either the CoG or the STG of H. americanus. We therefore tested a series of other histamine receptor antagonists, and found that the responses to puffs of histamine were blocked most effectively by a cocktail of two antagonists, the H 1 receptor antagonist transtriprolidine hydrochloride (Tocris Bioscience, Ellisville, MO, USA; catalog no.…”

“…0662) and the H 2 receptor antagonist ranitidine hydrochloride (Tocris Bioscience; catalog no. 1967), both of which have been shown to block some crustacean histamine receptors (Callaway and Stuart, 1989;Cebada and García, 2007); the cocktail consisted of 10 −6 to 5×10…”

GABA‐, histamine‐, and FMRFamide‐immunoreactivity in the visual, vestibular and central nervous systems ofHermissenda crassicornis

“…FMRFamide-IR in P. japonica (Ohsuga et al, 2000) and now H. crassicornis. Finally, based on evidence from arthropods, it is possible that some of the physiological experiments implicating GABA as the neurotransmitter in the hair cells could have been a consequence of GABA activating HA-gated chloride channels (McClintock & Ache, 1989;Cebada & García, 2007). This cross-activation has been described twice, although in both cases it was not susceptible to bicuculline (Kita, Irie, Nomura, Ozoe, & Ozoe, 2017;Gisselmann, Plonka, Pusch, & Hatt, 2004…”

Interactions between accumulated copper, bacterial community structure and histamine levels in crayfish meat during storage

“…Ingestion of food containing histamine can cause histamine poisoning with allergic‐like symptoms such as nausea, vomiting, diarrhea, hives, itching, red rash and hypotension . In crustaceans, histamine may not only be produced by bacteria, but is also naturally present and acts as a neurotransmitter . The United States Food and Drug Administration (US FDA) has set the maximum level of histamine in seafood as 50 µg g −1 (wet weight)…”