Histamine Release From Blood Cells in Anaphylaxis in Vitro

Katz, Gerhard

doi:10.1126/science.91.2357.221

Cited by 82 publications

(19 citation statements)

References 4 publications

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“…The demonstration that histamine is released during antigen-antibody interaction is in agreement with the findings of many previous investigators (9,10,17).…”

Section: Discussionsupporting

confidence: 81%

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Serotonin and Histamine Release During Anaphylaxis in the Rabbit

Waalkes¹,

Weissbach²,

Bozicevich³

et al. 1957

J. Clin. Invest.

164

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The release of physiologically active substances in animal tissues during anaphylaxis has been a subject of considerable interest for many years. Most of this interest has centered on the release of histamine from a bound to a free form, the latter being capable of producing marked physiological changes (1). However, despite the toxicity which can be attributed to histamine, attempts to explain all the alterations seen in anaphylaxis on the basis of histamine release alone have been unsatisfactory (2).Many of the effects produced by serotonin (5-hydroxytryptamine) in animals (3) resemble the manifestations of hypersensitivity reactions. In addition, patients with malignant carcinoid, a syndrome (4) in which there is present an excessive amount of serotonin, frequently show symptoms resembling those of an allergic process.. These findings have suggested that this amine may play a role in anaphylaxis and other allergic phenomena.Serotonin is found in the blood platelets of animals and man (5). Anaphylaxis is known to produce profound changes in these blood cells (6). Recently, Humphrey and Jaques (7) showed that both serotonin and histamine were released from platelets upon addition of purified antigen and antibody to normal rabbit platelets suspended in plasma. Also, Herxheimer (8) reported that in guinea pigs an interrelationship exists between desensitization and tolerance to serotonin.The present study was designed to determine whether serotonin is released during anaphylaxis. The experiments outlined below for serotonin are similar to those that were previously used to demonstrate the changes in blood histamine which occur during anaphylactic shock (9-12). Simultaneous determinations for both serotonin and histamine were carried out for comparative purposes. In this work, it has been shown that both amines appear in a free form in the plasma when a specific antigen is injected into sensitized rabbits. MATERIALS AND METHODSThe rabbit was chosen for these experiments for several reasons: (a) the large amounts of both serotonin and histamine carried in the blood platelets, (b) the ease of obtaining serial blood samples, and (c) the ability of the rabbit to produce adequate amounts of antibody.Male rabbits (1.5 Kg.) were sensitized to either egg albumin or horse serum. Those sensitized to egg albumin were given 10 ml. of a 10 per cent solution in saline intraperitoneally every other day for four injections. Those sensitized to horse serum were given 1 ml. of the undiluted serum intraperitoneally every day for six days. Ater three to five weeks, the rabbits were used as indicated. At this time, complement fixation antibody titers were high.Siliconized glassware was employed wherever whole blood had to be handled. Blood for the in ritro studies was obtained by intracardiac puncture. Blood samples for the in vivo studies were obtained by means of a plastic tube inserted into the carotid artery.Both serotonin and histamine were determined in all samples of plasma and whole blood. For plasma, 2 ml. were treated accord...

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“…The demonstration that histamine is released during antigen-antibody interaction is in agreement with the findings of many previous investigators (9,10,17).…”

Section: Discussionsupporting

confidence: 81%

“…The experiments outlined below for serotonin are similar to those that were previously used to demonstrate the changes in blood histamine which occur during anaphylactic shock (9)(10)(11)(12). Simultaneous determinations for both serotonin and histamine were carried out for comparative purposes.…”

mentioning

confidence: 77%

Serotonin and Histamine Release During Anaphylaxis in the Rabbit

Waalkes¹,

Weissbach²,

Bozicevich³

et al. 1957

J. Clin. Invest.

164

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show abstract

“…Taken together, the following observations strongly suggest that certain isoantibodies may, in causing hemolysis, provoke the release of histamine, serotonin and possibly other related substances from leukocytes and platelets: 1) the finding of others (56)(57)(58) that antigen-antibody reactions with the probable involvement of complement cause a release of histamine and of serotonin from leukocytes, platelets and certain other cells; 2) the observation of others (11) (60) and may reasonably be attributed to the sequence of events postulated to occur during hemolysis by isohemolysins.…”

Section: Dermal Responses To Immune Hemolysismentioning

confidence: 99%

“…Serotonin has been shown to cause edema in animals and to account for much of the edema provoked by foreign proteins (55). Furthermore, it has been shown that serotonin (56), as well as histamine (57), is released when antigen-antibody reactions occur in the presence of blood cells in vitro, and that in the case of serotonin, platelets appear to be the cellular source and complement is apparently involved (56). In rabbits, anaphylaxis is associated with immediate rises in plasma levels of serotonin and histamine, and concomitant falls have been recorded in the levels of these substances in blood cells (58).…”

Section: Dermal Responses To Immune Hemolysismentioning

confidence: 99%

The Destruction of Red Cells by Antibodies in Man. II. Pyrogenic, Leukocytic and Dermal Responses to Immune Hemolysis1

Jandl¹,

Tomlinson²

1958

J. Clin. Invest.

105

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“…The histamine in rabbit blood is contained mostly in the pla telet fraction (22) and can be released into the plasma by various agents or circumstances which injure the histamine-rich formed elements, such as trypsin and papain (4,5,25,26), peptone (9), the clotting of blood (1,2,28), the antigen-antibody reaction (11), etc. The question of just how these various agents, particularly antigen-antibody, accomplished the release of histamine is very important, but largely unanswered.…”

mentioning

confidence: 99%

The Mechanism of Histamine Release in Rabbit Blood

McIntire

1957

Int Arch Allergy Immunol

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Histamine Release From Blood Cells in Anaphylaxis in Vitro

Cited by 82 publications

References 4 publications

Serotonin and Histamine Release During Anaphylaxis in the Rabbit

Serotonin and Histamine Release During Anaphylaxis in the Rabbit

The Destruction of Red Cells by Antibodies in Man. II. Pyrogenic, Leukocytic and Dermal Responses to Immune Hemolysis1

The Mechanism of Histamine Release in Rabbit Blood

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