Histamine Release From Isolated Rat Mast Cells in Metal Ion-Free Medium

Yamasaki, Hidemasa; Sugiyama, Katsumi

doi:10.1254/jjp.22.325

Cited by 10 publications

(6 citation statements)

References 9 publications

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“…keeping with the results of Thon & Uvnas (1967) and Yamasaki & Sugiyama (1972), there was still a significant, corrected release of histamine in the absence of any added metal cations. The spontaneous release of histamine was verv high uLnder these conditions but was mUch reduced by low concentrations of divalent cations.…”

supporting

confidence: 86%

The Effect of Alkaline Earth Cations on the Release of Histamine From Rat Peritoneal Mast Cells Treated With Compound 48/80 and Peptide 401

Atkinson

Ennis

Pearce

1979

British J Pharmacology

140

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1 Extracellular calcium ions have a dual effect on the release of histamine from rat peritoneal mast cells treated with compound 48/80 and peptide 401. The release is either potentiated or inhibited according to the relative concentrations of ion and inducer. 2 Strontium similarly potentiates the release produced by optimal concentrations of inducer but higher concentrations are required than in the case of calcium. Strontium is markedly less inhibitory than calcium. 3 Mast cells may be depleted of intracellular calcium by incubation for short periods with the chelating agent, ethylenediamine tetraacetic acid (EDTA). They thereby become unresponsive to compound 48/80 and peptide 401 unless calcium is reintroduced into the incubation medium. Strontium and barium, but not magnesium, will substitute for calcium in this system. Barium additionally produces a marked release of histamine even in the absence of inducer. Pretreatment with the ionophore A23187 similarly inhibits the subsequent response to peptide 401 in divalent cation-free medium. This inhibition is reversed on the reintroduction of calcium. 4 Compound 48/80 and peptide 401 release histamine from mast cells incubated in isotonic sucrose in the complete absence of added metal ions. However, the corrected release under these conditions is potentiated by both mono and divalent cations. 5 On the basis of these results, the possible mechanism of action of the basic releasing agents and their usefulness as models for studying histamine secretion is discussed.

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supporting

confidence: 86%

The Effect of Alkaline Earth Cations on the Release of Histamine From Rat Peritoneal Mast Cells Treated With Compound 48/80 and Peptide 401

Atkinson

Ennis

Pearce

1979

British J Pharmacology

140

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“…Spontaneous histamine re lease was 5.2 ± 2.5% (n = 75). Basic secreta gogues are able to release histamine in the absence of extracellular calcium or in the pres ence of a low concentration of calcium (3,14,15). Under our conditions, the values of hista mine release percentage induced by compound 48/80, bradykinin, PEI6 and substance P in the presence of 0.3 mM calcium were 62.2 ± 3.2 (n = 16), 42.9 ± 3.2 (n = 8), 34.7 ± 3.1 (n = 10) and 24.1 ± 1.6 (n = 8), respec tively.…”

Section: Preparation Of Purified Mast Cellsmentioning

confidence: 99%

Sugar-Specific Inhibitory Effects of Wheat Germ Agglutinin and Phytohemagglutinin-E4 on Histamine Release Induced by Basic Secretagogues from Rat Peritoneal Mast Cells and Their Possible Action Sites

Suzuki-Nishimura

Nagaya

Matsuda

et al. 1991

Japanese Journal of Pharmacology

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ABSTRACT-The histamine release induced by compound 48/80, bradykinin or polyethylenimine with a molecular weight of 600 (PEI6) was inhibited by wheat germ agglutinin (WGA) and phytohemagglutinin E-subunits (PHA-E4), and the inhibition was specifically reversed by N-acetyl glucosamine and N-acetyl galactosamine, respec tively. Concanavalin A (Con A) and phytohemagglutinin L-subunits (PHA-L4) did not inhibit the histamine release induced by compound 48/80, bradykinin or PEI6. The histamine release induced by substance P was also inhibited sugar-specifically by WGA and PHA-E4. The binding sites for compound 48/80, bradykinin, PEI6 and sub stance P, therefore, seemed to especially overlap each other. These binding sites were found to be glycoproteins having affinities to WGA and PHA-E4, but not to Con A and PHA-L4. The binding of WGA and PHA-E4 to the glycoproteins resulted in inhibition of the interaction between the basic secretagogues including bradykinin and substance P and their binding sites on the mast cells. The bindings of five lectins to mast cell glycoproteins were examined by lectin-blotting. Several glycoproteins, which had specific affinities to WGA and PHA-E4, but not to Con A and PHA-L4 were de tected. We assumed that the binding sites for basic secretagogues which are coupled with histamine-releasing mechanisms exist among these glycoproteins. A 41-kDa pro tein (a-subunit of pertussis toxin-sensitive G protein) was not detected by WGA, sug gesting that the binding sites for the basic secretagogues were not G proteins.Stimulus-secretion coupling mechanisms in rat peritoneal mast cells are thought to involve G proteins, because GTP-analogues enhanced histamine release (1). Moreover, histamine re lease induced by compound 48/80 or brady kinin is inhibited by pertussis toxin, but that induced by antigen or anti-IgE is not (2-4). We found that the histamine release induced by polyethylenimine (MW = 600, PEI6) was inhibited by pertussis toxin (5). Compound 48/80, bradykinin and polyethylenimine are all basic secretagogues. There is, however, no similarity in their chemical structures. The ac tion sites for compound 48/80 on the plasma

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“…Unlike IgE-depend ent activation, the activation by non-immunologic stimuli is independent of IgE receptors, extracellular calcium and phosphatidylserine (10), but is dependent on Gi-like G protein activation (11)(12)(13)(14). Compound 48/80, substance P and bradykinin are basic secretagogues.…”

mentioning

confidence: 99%

Inhibitory Effects of Sialic Acid- or N-Acetylglucosamine-Specific Lectins on Histamine Release Induced by Compound 48/80, Bradykinin and a Polyethylenimine in Rat Peritoneal Mast Cells

Matsuda

Niitsuma

Uchida

et al. 1994

Japanese Journal of Pharmacology

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ABSTRACT-The effects of seven lectins with various sugar-specificities on histamine release from rat peritoneal mast cells induced by non-immunologic stimuli were studied. The non-immunologic stimuli used were three basic secretagogues, compound 48/80, bradykinin and PEI6 (polyethylenimine with a molecular weight of 600). In this study, we observed inhibition of the histamine release by Macckia amurensis mitogen and Solanum tuberosum agglutinin (100 tg/ml at 371C for 10 min), which are specific for sialic acid-a2,3 N-acetyl galactosamine (Siaa2,3Ga1NAc) and N-acetyl glucosamine (G1cNAc) oligomers, respectively. The effects of Phytolacca americana mitogen and Sambucus sieboldiana agglutinin were different. Three lectins specific for mucin type oligosaccharides inhibited the histamine release induced by compound 48/80 but not that induced by bradykinin or PEI6. Since bradykinin and PEI6 additively enhanced the histamine release in duced by compound 48/80, they partially shared the same signalling pathways. Glycoproteins with bisecting GIcNAc and Sia residues, as described previously (Jpn. J. Pharmacol. 57, 79-90, 1991), seemed to be one of the action sites for compound 48/80, bradykinin and PEI6. In addition to the direct activation of the per tussis toxin-sensitive G proteins, we propose another mechanism of non-immunologic stimuli via specific glycoproteins on rat peritoneal mast cells. The apparent sugar residues involved were asparagine-linked oligosaccharides with Sia (especially Siaa2,3Gal), G1cNAc oligomers and/or bisecting G1cNAc.

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Histamine Release From Isolated Rat Mast Cells in Metal Ion-Free Medium

Cited by 10 publications

References 9 publications

The Effect of Alkaline Earth Cations on the Release of Histamine From Rat Peritoneal Mast Cells Treated With Compound 48/80 and Peptide 401

The Effect of Alkaline Earth Cations on the Release of Histamine From Rat Peritoneal Mast Cells Treated With Compound 48/80 and Peptide 401

Sugar-Specific Inhibitory Effects of Wheat Germ Agglutinin and Phytohemagglutinin-E4 on Histamine Release Induced by Basic Secretagogues from Rat Peritoneal Mast Cells and Their Possible Action Sites

Inhibitory Effects of Sialic Acid- or N-Acetylglucosamine-Specific Lectins on Histamine Release Induced by Compound 48/80, Bradykinin and a Polyethylenimine in Rat Peritoneal Mast Cells

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