Histamine-Releasing Activity of Endogenous Peptides on Mast Cells Derived from Different Sites and Species

Arock, Michel; Devillier, Philippe; Luffau, G.; Guillosson, Jean‐Jacques; Renoux, M

doi:10.1159/000234951

Cited by 15 publications

(2 citation statements)

References 28 publications

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“…Culture of mast cells from bone marrow takes 14 to 15 weeks to produce a 99% pure population. Mast cells can also be isolated from various tissues (e.g., airways, intestine) or from the peritoneal cavity (Arock et al, 1989).…”

Section: Mast Cellsmentioning

confidence: 99%

The Use ofIn VitroSystems for Evaluating Immunotoxicity: The Report and Recommendations of an ECVAM Workshop

Gennari

Ban

Braun

et al. 2005

Journal of Immunotoxicology

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This is the report of a workshop organised by the European Centre for the Validation of Alternative Methods (ECVAM). ECVAM's main goal, as defined in 1993 by its Scientific Advisory Committee, is to promote the scientific and regulatory acceptance of alternative methods that are of importance to the biosciences and which replace, reduce or refine the use of laboratory animals. One of the first priorities set by ECVAM was the implementation of procedures that would enable it to become well informed about the state-of-the-art of non-animal test development and validation, and the potential for the possible incorporation of alternative tests into regulatory procedures. It was decided that this would be best achieved by the organization of ECVAM workshops on specific topics, at which small groups of invited experts would review the current status of various types of in vitro tests and their potential uses, and make recommendations about the best ways forward (Anonymous, 1994). The workshop on "The use of in vitro systems for evaluating Immunotoxicity" was held at ECVAM (Ispra), Italy, on 24th-26th November 2003. The participants represented academia, national organizations, international regulatory bodies and industry. The aim of the workshop was to review the state-of-the-art in the field of in vitro immunotoxicology, and to develop strategies towards the replacement of in vivo testing. At the end of this report are listed the recommendations that should be considered for prevalidation and validation of relevant and reliable procedures, that could replace the use of animals in chemical and cosmetics toxicity testing.

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Section: Mast Cellsmentioning

confidence: 99%

The Use ofIn VitroSystems for Evaluating Immunotoxicity: The Report and Recommendations of an ECVAM Workshop

Gennari

Ban

Braun

et al. 2005

Journal of Immunotoxicology

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“…Enhancement of serotonin release by bradykinin. A wide variety of substances have been reported to induce histamine release or to enhance the response of mast cells to substances that induce histamine release in vitro (1,6,17,21,27,29,30,31,35,48,50,52; for a review, see reference 49). Neuropeptides have also been suggested to trigger mast cells to release histamine in vivo (10).…”

Section: Resultsmentioning

confidence: 99%

Effects of staphylococcal enterotoxin B on rodent mast cells

et al. 1992

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Staphylococcal enterotoxin B (SEB) was tested in rodent mast cell cultures for the release of serotonin. Both rat RBL-2H3 mast cells and murine peritoneal cells released serotonin after SEB stimulation in culture. Release of serotonin in RBL-2H3 cells depended on the concentration of SEB; an appreciable release was seen at 50 ,ug/ml. The release of serotonin was not due to cell death. Serotonin release could be enhanced by bradykinin but not by vasoactive intestinal peptide, substance P, lipopolysaccharide from Salmonella typhimurium, the calcium ionophore A23187, acetylcholine, adenosine, 5-hydroxyeicosatetraenoic acid, indomethacin, or phorbol myristate acetate. SEB bound directly to the membrane of RBL-2H3 mast cells, and the SEB-binding site, the presumptive receptor, appeared to be a protein. The SEB receptor could not be capped under membrane-capping conditions, and serotonin release could not be enhanced by attempts to cross-link the receptor. These results suggest that mast cells may be an important cell type involved in SEB toxicosis and that release of serotonin may be enhanced by activation of the kinin-kallikrein system. The mechanism whereby staphylococcal enterotoxins cause toxic shock and other syndromes is unknown. Marrack and coworkers (32) suggested that the pathological reaction (weight loss and immunosuppression) in mice to staphylococcal enterotoxin B (SEB) is caused by T cells that are able to respond to SEB by mitosis. However, th6 toxicity of SEB and the related toxin staphylococcal enterotoxin C1 in monkeys is partially dissociated from the mitogellic effects of these molecules (45, 46). Thus, cells other t1ii T cells may also be involved in SEB toxicosis. For examplde there is some indirect evidence that mast cells may also contribute to some of the toxic reactions to SEB. Scheuber and coworkers (41) intradermally injected SEB into monkeys and found an immediate-type skin reaction that was presumably caused by mast cells because it was inhibitable by histamine inhibitors. We sought to study the interaction of mast cells with SEB. The RBL-2H3 rat leukemic cell line (3) and peritoneal mast cells from mice were used to determine whether SEB has a direct effect on mast cells. Most of the work was done with RBL-2H3 cells because of their homogeneity and the ease of obtaining large numbers of cells. In addition, there was no necessity to rule out immunoglobulin E-mediated phenomena (as there would be if fresh mast cells were used exclusively). We have found that rodent mast cells do in fact respond to SEB by releasing serotonin and that this response is enhanced by bradykinin. The SEB-binding site or receptor on mast cells appears to be a protein. MATERIALS AND METHODS Materials. RBL-2H3 cells (3) were the kind gift of Reuben Siraganian, National Cancer Institute, Bethesda, Md. SEB was produced by the method of Schantz and coworkers (40) and was obtained in lyophilized form from the U.S. Army Medical Research Institute of Infectious Diseases, Frederick, Md. Acetylcholine chloride, adenosine, b...

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Interactions of mast cells with the nervous system ?Recent advances

1992

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This article reviews recent advances in the understanding of mast cell-nervous system interactions. It is drawn largely from work published within the last ten years, and discusses the anatomical and biochemical evidence of a functional connection between mast cells and the nervous system, and the implications that such a relationship may have for normal and abnormal physiological functioning. Mast cells are found at varying levels of association with the nervous system; in CNS parenchyma (mainly thalamus), in connective tissue coverings (e.g. meninges, endoneurium), and in close apposition to peripheral nerve endings in a variety of tissues. There is, as yet, no clearly defined role for mast cells in nervous system function, or vice-versa, and it seems most likely that their interactions fulfil mutually modulatory roles. By extension, pathological situations where one of the partners in this relationship is overly stimulated may lead to a dysregulation of the other, and contribute to disease symptomatology.

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Histamine-Releasing Activity of Endogenous Peptides on Mast Cells Derived from Different Sites and Species

Cited by 15 publications

References 28 publications

The Use ofIn VitroSystems for Evaluating Immunotoxicity: The Report and Recommendations of an ECVAM Workshop

The Use ofIn VitroSystems for Evaluating Immunotoxicity: The Report and Recommendations of an ECVAM Workshop

Effects of staphylococcal enterotoxin B on rodent mast cells

Interactions of mast cells with the nervous system ?Recent advances

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