Histamine signalingviathe metabotropic receptorTrapped in endoderm 1regulates courtship initiation inDrosophila melanogaster

Sa, Zaki; Sandhu, Jasanvir; Rl, French

doi:10.1101/150680

Cited by 2 publications

(2 citation statements)

References 37 publications

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“…If any of Tre1 super family could be implicated in light sensitivity is still unknown. Interestingly histamine was reported to activate Tre1 receptor 19 . Whether it is the only GPCR ligand to be able to bind to Tre1 receptor is unknown.…”

Section: Discussionmentioning

confidence: 99%

Evolutionary history and origin of Tre1 superfamily shed light on its role in regulating blood brain barrier

Kubick

Klimovich²,

Bieńkowska

et al. 2021

Preprint

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Understanding how the evolutionary relationship between immune cells and the blood-brain is important to devise therapeutic strategies that can regulate their critical function. In vertebrates, immune cells follow either a paracellular or transcellular pathway to infiltrate the BBB. In drosophila glial cells form the BBB that regulates the access of immune-like cells to the drosophila brain. However, it is still not known which route immune-like cells follow to infiltrate the drosophila brain. In vertebrates, paracellular migration is dependent on PECAM1, while transcellular migration is dependent on the expression of CAV1. Interestingly drosophila genome lacks both genes. Tre1 superfamily (Tre1, Moody, and Dmel_CG4313) play a diverse role in regulating transepithelial migration in drosophila. However, its evolutionary history and origin are not yet known. We performed phylogenetic analysis, together with HH search, positive selection, and ancestral reconstruction to investigate the Tre1 family Interestingly we found that Tre1 exists in mollusks, insects, ambulacria, and sclaidphora. Moody is shown to be a more ancient protein and it existed since cnidaria emergence and has a homolog (GPCR84) in mammals. The third family member (Dmel_CG4313) only exists in insects. The origin of the family seems to be related to the rhodopsin-like family and in particular family α. We found that opsin is the nearest receptor to have a common ancestor with the Tre1 superfamily that seems to have diverged in sponges. We investigated the positive selection of the Tre1 family using PAML. Tre1 seems to have evolved under negative selection, whereas Moody has evolved during positive selection. The sites that we found under positive selection are Likely to play a role in the speciation of function in the case of Moody. We have identified an SH3, in Tre1 and, moody and Dmel_CG4313. Sh3 is known to play a fundamental role in regulating actin movement in a Rho-dependent manner. We suggest that Tre1 could be playing an important role in paracellular diapedesis in drosophila.

show abstract

Section: Discussionmentioning

confidence: 99%

Evolutionary history and origin of Tre1 superfamily shed light on its role in regulating blood brain barrier

Kubick

Klimovich²,

Bieńkowska

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…If any of the Tre1 family members could be implicated in light sensitivity is still unknown. Interestingly histamine was reported to activate the Tre1 receptor [26]. Whether it is the only GPCR ligand to be able to bind to the Tre1 receptor is unknown.…”

Section: Evolution History Of the Tre1 Familymentioning

confidence: 99%

Investigation of Evolutionary History and Origin of the Tre1 Family Suggests a Role in Regulating Hemocytes Cells Infiltration of the Blood–Brain Barrier

Kubick

Klimovich²,

Bieńkowska³

et al. 2021

Insects

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Understanding the evolutionary relationship between immune cells and the blood–brain barrier (BBB) is important to devise therapeutic strategies. In vertebrates, immune cells follow either a paracellular or a transcellular pathway to infiltrate the BBB. In Drosophila, glial cells form the BBB that regulates the access of hemocytes to the brain. However, it is still not known which diapedesis route hemocytes cells follow. In vertebrates, paracellular migration is dependent on PECAM1, while transcellular migration is dependent on the expression of CAV1. Interestingly Drosophila genome lacks both genes. Tre1 family (Tre1, moody, and Dmel_CG4313) play a diverse role in regulating transepithelial migration in Drosophila. However, its evolutionary history and origin are not yet known. We performed phylogenetic analysis, together with HH search, positive selection, and ancestral reconstruction to investigate the Tre1 family. We found that Tre1 exists in Mollusca, Arthropoda, Ambulacraria, and Scalidophora. moody is shown to be a more ancient protein and it has existed since Cnidaria emergence and has a homolog (e.g., GPCR84) in mammals. The third family member (Dmel_CG4313) seems to only exist in insects. The origin of the family seems to be related to the rhodopsin-like family and in particular family α. We found that opsin is the nearest receptor to have a common ancestor with the Tre1 family that has diverged in sponges. We investigated the positive selection of the Tre1 family using PAML. Tre1 seems to have evolved under negative selection, whereas moody has evolved during positive selection. The sites that we found under positive selection are likely to play a role in the speciation of function in the case of moody. We have identified an SH3 motif, in Tre1 and, moody and Dmel_CG4313. SH3 is known to play a fundamental role in regulating actin movement in a Rho-dependent manner in PECAM1. Our results suggest that the Tre1 family could be playing an important role in paracellular diapedesis in Drosophila.

show abstract

Histamine signalingviathe metabotropic receptorTrapped in endoderm 1regulates courtship initiation inDrosophila melanogaster

Cited by 2 publications

References 37 publications

Evolutionary history and origin of Tre1 superfamily shed light on its role in regulating blood brain barrier

Evolutionary history and origin of Tre1 superfamily shed light on its role in regulating blood brain barrier

Investigation of Evolutionary History and Origin of the Tre1 Family Suggests a Role in Regulating Hemocytes Cells Infiltration of the Blood–Brain Barrier

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