Histidinemia: Studies on histidase activity in stratum corneum

Speech and Language Disorders in Histidinaemia and other Amino Acid Disturbances

“…Two-dimensional thin layer chromatography of histidine and imidazoles with the same detection (Wadman et al, 1967) and quantitative estimation with liquid column chromatography on amino acid analyzers using short programmes were used for confirmatory studies. Skin histidase activity was measured according to Kihara and Boggs (1968). In loading test with L-histidine (100rag kg weight-1) blood and urine were taken at intervals of 0, 60, 120, 180, 240 and 360rains and changes in speech capacity were evaluated using the Lee effect method 60 rain after the load.…”

Speech Disorders Associated with Histidinemia and Other Hereditary Disorders of Amino Acid Metabolism

“…1980], Qualitative assessment of dermatic histidase was made by means of one-dimension descendant chro matography according to Levy et al [1974] from a skin homogenate. Quantitative assessment of the activity of dermatic histidase was made front a skin homoge nate according to Kihara et al [1969], The toleration test with /.-histidine after the ad ministration of 100 mg of pure amino acid per kilo gram of physical weight was made according to llyà-nek [1980], Statistical evaluation was made by means of the Fischer test.…”

“…Blood exami nation did not prove an increased quantity of histidine and its derivatives in these persons. Skin examination using the method of Levy et al [1974] for assessing the activity of der matic histidase failed to detect a decrease in enzymatic activity, even by applying the method of Kihara et al [1969].…”