Histochemical, biochemical, and contractile properties of red, white, and intermediate fibers

Barnard, R. James; Vr, Edgerton; Furukawa, Tatsuo; Peter, John

doi:10.1152/ajplegacy.1971.220.2.410

Cited by 368 publications

(112 citation statements)

References 8 publications

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“…20,29,30 Finally, in the present study UCP2 and UCP3 mRNA levels on the high-fat diet, as well as the diet-induced changes in UCP2, UCP3L and UCP3T mRNA were correlated with the amount of type IIA ®bers. Type IIA ®bers are characterized by a high capacity for both carbohydrate and fat oxidation 31 and it can be suggested that these ®bers have a high capacity to switch from carbohydrate to fat oxidation when a high-fat diet is consumed. Therefore, these data, together with data from Samec et al 8,15 who showed similar ®ndings in rats, are in accordance with a role for UCPs in the metabolism of fatty acids, but further studies are clearly needed to explore the physiological function of human uncoupling proteins.…”

Section: Discussionmentioning

confidence: 99%

Fiber type dependent upregulation of human skeletal muscle UCP2 and UCP3 mRNA expression by high-fat diet

et al. 2001

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OBJECTIVE:To test the hypothesis that consumption of a high-fat diet leads to an increase in UCP mRNA expression in human skeletal muscle. In a group of endurance athletes, with a range in ®ber type distribution, we hypothesized that the effect of the high-fat diet on UCP2 and UCP3 mRNA expression is more pronounced in muscle ®bers which are known to have a high capacity to shift from carbohydrate to fat oxidation (type IIA ®bers). DESIGN: Ten healthy trained athletes (®ve males, ®ve females) consumed a low-fat diet (17 AE 0.9 en% of fat) and high-fat diet (41.4 AE 1.4 en% fat) for 4 weeks, separated by a 4 week wash-out period. Muscle biopsies were collected at the end of both dietary periods. MEASUREMENTS: Using RT-PCR, levels of UCP2 and UCP3 mRNA expression were measured and the percentage of type I, IIA and IIB ®bers were determined using the myo®brillar ATPase method in all subjects. RESULTS: UCP3L mRNA expression tended to be higher on the high-fat diet, an effect which reached signi®cance when only males were considered (P 0.037). Furthermore, diet-induced change in mRNA expression of UCP3T (r: 0.66, P 0.037), UCP3L (r: 0.61, P 0.06) and UCP2 (r: 0.70, P 0.025), but not UCP3S, correlated signi®cantly with percentage dietary fat on the high-fat diet. Plasma FFA levels were not different during the two diets. Finally, the percentage of type IIA ®bers was positively correlated with the diet-induced change in mRNA expression for UCP2 (r: 0.7, P 0.03), UCP3L (r: 0.73, P 0.016) and UCP3T (r: 0.68, P 0.03) but not with UCP3S (r: 0.06, NS). CONCLUSION: UCP2 and UCP3 mRNAs are upregulated by a high-fat diet. This upregulation is more pronounced in humans with high proportions of type IIA ®bers, suggesting a role for UCPs in lipid utilization.

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Section: Discussionmentioning

confidence: 99%

Fiber type dependent upregulation of human skeletal muscle UCP2 and UCP3 mRNA expression by high-fat diet

et al. 2001

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“…In birds, type III fibres (slow tonic and multi innervated fibres) can also be distinguished. Combining metabolic enzyme-based and mATPase-based histochemical methods, Barnard et al [6], and Peter et al [61] distinguished three types of fibres ( Fig. 1C) : slow-twitch oxidative (SO), fast-twitch oxido-glycolytic (FOG) and fasttwitch glycolytic (FG).…”

Section: Myofibre Typingmentioning

confidence: 99%

Muscle fibre ontogenesis in farm animal species

et al. 2002

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“…Celui-ci est un facteur limitant du niveau de l'activité, lié en partie à sa capacité de transport par la circulation sanguine, en partie à son taux d'utilisation par les mitochondries. Seule parmi les enzymes de la glycolyse, l'activité de l'hexokinase est plus élevée dans les muscles rouges que dans les muscles blancs (Peter etal., 1968 ;Burleigh et Schimke, 1968 ; (Barnard et al, 1971 ;Fardeau, 1973 ;Edgerton et al, 1975 ;Vigneron et al, 1976 (Barany et al, 1965 ;Sreter et a/., 1966 ;Barany, 1967 ;Samaha et al, 1970 ;Marston et Taylor, 1980 (Hoh, 1979 ;Lowey et al, 1982 ;Whalen et al, 1982 (Barton etal., 1985 ; Wieczorek et al, 1985 ;revue de Weydert, 1988 Eisenberg, 1980). Les troponines sont des protéines globulaires dénommées troponine C (PM = 18 000), 1 (PM = 21 000-24 000) et T (PM = 31 000-36 000) présen-tant une grande affinité respectivement pour le CA ++ , pour l'actine et pour la tropomyosine (Mannherz et Goody, 1976).…”

Section: A) Propriétés Des Fibres Musculairesunclassified

Propriétés des fibres musculaires squelettiques. 1. Influence de l'innervation motrice

Bacou¹,

Vigneron²

1988

Reprod. Nutr. Dévelop.

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Histochemical, biochemical, and contractile properties of red, white, and intermediate fibers

Cited by 368 publications

References 8 publications

Fiber type dependent upregulation of human skeletal muscle UCP2 and UCP3 mRNA expression by high-fat diet

Fiber type dependent upregulation of human skeletal muscle UCP2 and UCP3 mRNA expression by high-fat diet

Muscle fibre ontogenesis in farm animal species

Propriétés des fibres musculaires squelettiques. 1. Influence de l'innervation motrice

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