Histochemical Changes Associated with Aging: IV. Liver, Brain, and Kidney in the Rat

Lowry, Oliver H.; Hastings, A. Baird; McCay, C. M.; Brown, Andrea N.

doi:10.1093/geronj/1.3_part_1.345

Cited by 53 publications

(30 citation statements)

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“…Values agrees with the actual sodium space based on estimates of brain sodium concentration (Lowry et al 1946;Reed et al 1964). Figs The results ofthe peel-off analysis are as follows.…”

Section: Victor Levin and Clifford S Patlaksupporting

confidence: 74%

“…2 and 3 are the respective semilogarithmic plots for "Na brain and nerve exchange. The ordinate is the function 1-24Na Space/TES, where TES is the 'theoretical equilibrium space' for sodium in brain or nerve which should be equal to the ratio of the chemically determined value of the tissue sodium concentration to the plasma water sodium concentration (Ames, Sakanove & Endo, 1964;Ferguson'& Woodbury, 1969;Hanig & Aprison, 1967;Koch & Woodbury, 1960;Lowry et al' 1946;Luciano, 1968;Reed et at. 1964).…”

Section: Victor Levin and Clifford S Patlakmentioning

confidence: 99%

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A compartmental analysis of ²⁴Na kinetics in rat cerebrum, sciatic nerve and cerebrospinal fluid

Levin¹,

Patlak²

1972

The Journal of Physiology

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SUMMARY1. The kinetics of 24Na exchange in rat cerebrospinal fluid, brain and sciatic nerve were studied during steady-state plasma isotope conditions. The entrance of 24Na into brain and sciatic nerve was found to fit a three compartmental model. Using newly derived equations for compartmental analysis, the first compartment 24Na space of brain was found to be about 22 % and the second compartment space about 6 %; the corresponding sciatic nerve spaces were 26 and 11 %, respectively. In the discussion, arguments are presented that suggest that the first compartment is the extracellular space (ECS) and the second compartment is the intracellular space. The tj for compartmental equilibration between plasma and brain ECS was 2 hr and from ECS to intracellular space 14 hr; in nerve the respective ti's were i hr and 10 hr.2. A third compartment of 18 % was found in sciatic nerve which was interpreted to be anatomically confined to the epi-and perineurium; in brain the corresponding space was 2 % and was felt to be anatomically and kinetically an inactive closed compartment in parallel with the blood.3. There were two tj's for plasma-c.s.f. 24Na equilibration of less than 10 min and 2 hr.4. Profiles of brain, from cortex to ventricle, at 6 min, demonstrated a gradient of decreasing 24Na radioactivity from ventricle to cortex.

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“…Values agrees with the actual sodium space based on estimates of brain sodium concentration (Lowry et al 1946;Reed et al 1964). Figs The results ofthe peel-off analysis are as follows.…”

Section: Victor Levin and Clifford S Patlaksupporting

confidence: 74%

Section: Victor Levin and Clifford S Patlakmentioning

confidence: 99%

A compartmental analysis of ²⁴Na kinetics in rat cerebrum, sciatic nerve and cerebrospinal fluid

Levin¹,

Patlak²

1972

The Journal of Physiology

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“…From the Department of Physiology, University College London (Received 26 April 1954) A number of quantitative studies have been made of the growth of collagen after birth in various mammalian organs (Lowry, Hastings, McCay & Brown, 1946;Elster & Lowry, 1950), and in adult organs subjected to stimuli to growth (Abercrombie & Johnson, 1946, 1947Harkness, 1952;Harkness & Harkness, 1954b). No similar studies appear to have been made on the products of gestation, though they have been made on the chick embryo (Neuman, 1950).…”

Section: By Margaret L R Harkness and R D Harknessmentioning

confidence: 99%

The growth of collagen in the foetus, placenta and foetal membranes of the rat

Harkness¹,

Harkness²

1955

The Journal of Physiology

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. We have now made quantitative observations on the collagen content of the placenta, membranes and foetus of the rat during pregnancy. In all cases collagen content increased more rapidly than wet or dry weight. In the course of this work we also studied the quantity of amniotic fluid present at different times of pregnancy. METHODSAlbino rats ofthe local strain were used. Pregnancy was timed to ± 1 day by the method previously used (Harkness & Harkness, 1954b). Collagen was estimated by the method of Neuman & Logan (1950) with minor modifications (Harkness & Harkness, 1954a). Dryweightoftissueswas measured after drying over phosphorus pentoxide in vacuo. Total nitrogen was measured by the Kjeldahl procedure; digestion was done by the method of Chibnall, Rees & Williams (1943) and the ammonia was estimated after distillation into boric acid containing bromeresol green-methyl red indicator (Conway, 1947). The placenta and foetuses were homogenized in a small blendor of the Waring type. This did not break up all the individual bones of the older foetuses but we found that this did not appreciably affect the estimation. For both foetuses and placentas, judged by the hydroxyproline content of the residuum, the amount of collagen left after the usual aliquot was removed for analysis after autoclaving (Harkness & Harkness, 1954a) corresponded to the amount expected from the size of the aliquot, i.e. if any collagen in the bones remained undissolved after autoclaving the amount was too small to affect the results appreciably. Tyrosine estimations were made by the method of Medes (1932) on a number of samples and the quantity was small enough to be neglected. The hydroxyproline values have been accepted without correction for tyrosine.The animals were killed by a blow on the head and breaking the neck. The whole uterus was removed and weighed. The horns were opened and the foetuses removed with their placentas and membranes enclosing the amniotic fluid intact. The foetuses were then separated from the placentas, umbilical cords and membranes. These structures, together with the foetuses and empty uterus, were weighed and the weight of amniotic fluid was obtained by difference from the original 15 PHYSIO. CXXVIII

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“…In extreme age there are small decreases in fat acid soluble phosplhorus, and potassium, with an increase in sodium (23).…”

Section: Changes In the Brainmentioning

confidence: 97%

“…There are studies that report a definite decrease in water content in the brain throughout the entire life span (22,23). This decrease varies somewhat with different species since there seems to be a remarkable correlation between physical and mental development at the time of birth and subsequent water loss.…”

Section: Changes In the Brainmentioning

confidence: 99%

Changes in Nervous System with Age

Wilcox¹

1956

Public Health Reports (1896-1970)

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Histochemical Changes Associated with Aging: IV. Liver, Brain, and Kidney in the Rat

Cited by 53 publications

References 0 publications

A compartmental analysis of ²⁴Na kinetics in rat cerebrum, sciatic nerve and cerebrospinal fluid

A compartmental analysis of ²⁴Na kinetics in rat cerebrum, sciatic nerve and cerebrospinal fluid

The growth of collagen in the foetus, placenta and foetal membranes of the rat

Changes in Nervous System with Age

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Histochemical Changes Associated with Aging: IV. Liver, Brain, and Kidney in the Rat

Cited by 53 publications

References 0 publications

A compartmental analysis of 24Na kinetics in rat cerebrum, sciatic nerve and cerebrospinal fluid

A compartmental analysis of 24Na kinetics in rat cerebrum, sciatic nerve and cerebrospinal fluid

The growth of collagen in the foetus, placenta and foetal membranes of the rat

Changes in Nervous System with Age

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Product

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A compartmental analysis of ²⁴Na kinetics in rat cerebrum, sciatic nerve and cerebrospinal fluid

A compartmental analysis of ²⁴Na kinetics in rat cerebrum, sciatic nerve and cerebrospinal fluid