Histochemical demonstration of carbonic anhydrase and in the pecten oculi of the fowl

Eichorn, Michael; Flügel, Cassandra

doi:10.1016/0014-4835(88)90031-0

Cited by 7 publications

(1 citation statement)

References 10 publications

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“…The presence of the microfolds determines an enormous enlargement of the inner and outer surfaces of the vessels. The demonstration of carbonic anhydrase activity in the apical and basal microfolds of the pectineal endothelial cells (Eichhorm and Flugel, 1988) indicates that the pecten may play a significant role in the intraocular pH regulation, as already suggested by Brach (1977) after experimental destruction of the pecten and by Gerhardt et al (1999) during its development. Furthermore, as it was shown that the pectineal endothelial cells possess an extraordinarily high amount of glucose transporter isoform‐1 (Gerhardt et al, 1999), it is possible to propose that in the budgerigar the folds region, characterized by the longest luminal and AM, may have a trophic role as a glucose provider to fuel the glycolysis of the avascular retina (Wolburg et al, 1999).…”

Section: Discussionmentioning

confidence: 74%

Morphological and Morphometric Study of the Pecten Oculi in the Budgerigar (Melopsittacus undulatus)

Micali

Pisani

Ventrici

et al. 2012

The Anatomical Record

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The pecten oculi is a highly vascular and pigmented organ placed in the vitreous body of the avian eye. As no data are currently available on the morphological organization of the pecten in the Psittaciformes, the pecten oculi of the budgerigar (Melopsittacus undulatus) was studied. The eyes from adult male budgerigars were examined by light, transmission, and scanning electron microscopy and a morphometric study on both light and transmission electron microscopy specimens was also performed in the different parts of the organ. In the budgerigar, the type of the pecten oculi was pleated. Its basal part had a cranio-caudal and postero-anterior course; its body consisted of 10-12-folds joined apically by a densely pigmented bridge. The pecten showed many capillaries, whose wall was thick and formed by pericytes and endothelial cells. These latter had a large number of microfolds, rectilinear on their luminal surface and tortuous on their abluminal surface. Interstitial pigment cells were placed among the capillaries, filled with melanin granules and showed many cytoplasmic processes. The morphometric analysis demonstrated significant differences among the three parts of the organ relative to the length of the endothelial processes and to the number and size of the pigment granules. The morphological and morphometric analysis showed that the bridge of the budgerigar, different from the other birds, had a large number of capillaries, so that this part of the organ could also play a trophic role for the retina in addition to the choriocapillaris. Anat Rec, 295:540-550, 2012. V V C 2012 Wiley Periodicals, Inc.

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Section: Discussionmentioning

confidence: 74%

Morphological and Morphometric Study of the Pecten Oculi in the Budgerigar (Melopsittacus undulatus)

Micali

Pisani

Ventrici

et al. 2012

The Anatomical Record

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Carbonic anhydrase activity is increased in retinal pigmented epithelium and choriocapillaris of RCS rats

Eichhorn

Schreckenberger

Tamm

et al. 1996

Graefe's Arch Clin Exp Ophthalmol

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Characterization of bovine ciliary pigmented epithelial cells in monolayer culture: An ultrastructural, enzyme histochemical and immunohistochemical study

Eichhorn

Bermbach

Dermietzel

et al. 1993

Graefe's Arch Clin Exp Ophthalmol

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Monolayer cultures of the pigmented epithelial (PE) cells derived from two regions of the pars plicata of bovine eyes were established and grown up to the third passage. After this passage, the cultures became senescent. During the first three passages, the PE cells lost their pigment granules but developed a distinct cellular polarity by forming junctional complexes at their apical cell portions and depositing basement membrane like material on their basal side. The junctional complexes were shown to be impermeable for horseradish peroxidase, suggesting that they contained tight junctions. Histochemically, the monolayer cells stained for carbonic anhydrase (CA) and Na+/K(+)-ATPase, enzymes involved in active fluid secretion. Staining for CA and Na+/K(+)-ATPase as well as for acid phosphatase and immunostaining for vimentin and actin of the cultured PE cells were comparable with that of PE cells in vivo. Therefore, PE monolayer cultures are considered to be a suitable model for experimental studies in vitro.

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Histochemical demonstration of carbonic anhydrase and in the pecten oculi of the fowl

Cited by 7 publications

References 10 publications

Morphological and Morphometric Study of the Pecten Oculi in the Budgerigar (Melopsittacus undulatus)

Morphological and Morphometric Study of the Pecten Oculi in the Budgerigar (Melopsittacus undulatus)

Carbonic anhydrase activity is increased in retinal pigmented epithelium and choriocapillaris of RCS rats

Characterization of bovine ciliary pigmented epithelial cells in monolayer culture: An ultrastructural, enzyme histochemical and immunohistochemical study

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