Histochemical demonstration of hydrochloric acid in the gastric tubules of teleosts using an in vivo prussian blue technique

Western, J. R. H.; Jennings, J. B.

doi:10.1016/0010-406x(70)90083-6

Cited by 23 publications

(8 citation statements)

References 10 publications

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“…As reported for reptiles (Ferri et al 1974), for amphibians (Sedar 1962) and for some fish (Western & Jennings 1970, Ling & Tan 1975, Noaillac-Depeyre & Gas, 1978 the cod gastric glands contain one type of cells only. As reported for reptiles (Ferri et al 1974), for amphibians (Sedar 1962) and for some fish (Western & Jennings 1970, Ling & Tan 1975, Noaillac-Depeyre & Gas, 1978 the cod gastric glands contain one type of cells only.…”

Section: Discussionsupporting

confidence: 53%

“…The structure and ultrastructure of the gastric glands of cod show similarities with those of other lower vertebrates. As reported for reptiles (Ferri et al 1974), for amphibians (Sedar 1962) and for some fish (Western & Jennings 1970, Ling & Tan 1975, Noaillac-Depeyre & Gas, 1978 the cod gastric glands contain one type of cells only. These cells have secretory granules or vesicles as well as a vast tubular system and we suggest that they have the function of secreting both digestive proenzymes and the acid.…”

Section: Discussionsupporting

confidence: 53%

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The ultrastructure of the gastric glands and its relation to induced secretory activity of cod, Gadus morhua (Day)

Mattisson¹,

Holstein²

1980

Acta Physiologica Scandinavica

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Light and electron microscopic studies have been performed on different parts of cod stomach. The studies have been concentrated on the gastric glands of the gastric mucosa. The glands consist of only one cell type. This cell contains an apically located, comprehensive tubular system as well as zymogen granules and therefore probably secretes both acid and zymogen. The high degree of development of certain organelles and their mutual localization shows a structural organization which appears suitable for an effective secretion. What appears to be intermediate organelle forms are common. When acid secretion is increased by continuous intramuscular infusion of histamine or of carbachol the most marked structural changes are increases in the size of the gland lumen and in the cell membrane facing the lumen. Histamine in particular causes the appearance of numerous long and slender cell processes in an enlarged gland lumen. When the acid secretion, increased by histamine, had reached a plateau, metiamide was infused. This histamine H2-receptor antagonist caused a decrease in acid secretion as well as in the size of the gland cell surface to values characteristic of untreated cod.

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Section: Discussionsupporting

confidence: 53%

Section: Discussionsupporting

confidence: 53%

The ultrastructure of the gastric glands and its relation to induced secretory activity of cod, Gadus morhua (Day)

Mattisson¹,

Holstein²

1980

Acta Physiologica Scandinavica

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“…Pepsinogen granules in the gastric gland cells were demonstrated by Adam's (1957) dimethylaminobenzaldehyde nitrite method for tryptophan. Hydrochloric acid in the gastric tubules was demonstrated by an in vivo Prussian blue technique (Western & Jennings, 1970).…”

Section: Methodsmentioning

confidence: 99%

Feeding and digestion in two cottid fishes, the freshwater Cottus gobio L. and the marine Enophrys bubalis (Euphrasen)

Western

1971

Journal of Fish Biology

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A comparative study of feeding and digestion in the freshwater Cuttus gobio and the marine Enoghrys bubnlis has been made. The digestion of live prey taken voluntarily, and of aqueous starch suspensions and of olive oil administered by stomach tube, are described at the macroscopic and histological level. Particular attention has been paid to the food capacity of the gut, the mechanical treatment of the food, and the role of mucus in the alimentary tract.Digestion, which is similar in the two species, is divided into three distinct phases, corresponding to the three morphologically separate regions of the post-pharyngeal gut. The relative roles of each of the three regions in the digestion and absorption of food are assessed.No modification of gut structure or function in either species directly attributable to environmental salinity has been detected. The evidence suggests that the faster growth rate of the marine species may be due to the greater digestive-absorptive capacity of its digestive system.

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“…The small boost of enzyme secretion after 24th h prior to feeding to finish off the remaining food implied that enzyme secretion might be regulated by stomach distension as proposed by Smit (1968) in frogs (Rana sp. ), and Western and Jennings (1970) in cottids (Cottus gobio) and brown trout (Salmo trutta). A smaller bolus of food created less distention of stomach wall which resulted in less enzyme secretion.…”

Section: Discussionmentioning

confidence: 99%

Gastric Emptying and the Enzymatic Activity in the Stomach of Amphiprion ocellaris Fed on Artificial Diet

Khoo

Das²,

Ghaffar

2019

JSM

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This study aims to elucidate the gastric emptying process of clownfish fed on artificial diet using two gastric evacuation models and to determine the pepsin activity in the digestion process in relation to feeding time. Regression analysis was used to evaluate the adequacy of 2 models; Anderson's [S t in describing the gastric emptying rate. Grove's model provided a better fit with higher r 2 value, with the calculated parameters of maximum meal size at time 0 (S 0 ) = 0.195 g and gastric emptying rate (K) = 0.0165 g h -1 . There was no initial delay phase as predicted and the evacuation followed a curve. Pepsin activity in the stomach showed rapid responses to food intake, where activity was detected at 1 h after feeding and reached its peak at 2 h after feeding. Pepsin activity decreased since then until the 12th h after feeding where it reached the lowest point. An increase of pepsin activity was detected later, where a small boost was detected at 24 h after feeding to digest the remaining food item in the stomach before the pepsin secretion decreased and maintained at pre-feeding level. Fast response of digestive enzyme in stomach implied that clownfish is equipped to utilize infrequent and irregular meals effectively. ABSTRAK Kajian ini bertujuan untuk menjelaskan proses pengosongan perut ikan badut yang diberi diet buatan dengan menggunakan dua model pengosongan gastrik dan untuk menentukan hubungan antara aktiviti enzim pepsin dalam proses pencernaan dengan masa selepas makan. Analisis regresi digunakan untuk menentukan ketepatan dua model; model Anderson [S t = S o (1-S o (α-1) ρ(1-α)t) 1/(1-α) + ξ] dan model Grove [S t = (S oα -αKt) 1/α ], dalam penentuan kadar pengosongan perut selepas makan. Model Grove lebih sesuai kerana mempunyai nilai r yang lebih tinggi dengan parameter yang dikira merupakan saiz hidangan maksimum pada masa 0(S 0 ) = 0.195 g dan kadar pengosongan perut (K) = 0.0165 g per jam. Tiada fasa lewat pada permulaan proses penghadaman seperti yang dianggarkan dan pengosongan perut adalah sejajar dengan lengkungan. Aktiviti enzim pepsin dalam perut menunjukkan tindak balas yang pantas terhadap pengambilan makanan dengan aktiviti pepsin dikesan seawal 1 jam selepas makan dan mencapai kemuncaknya pada masa 2 jam selepas makan. Aktiviti pepsin kemudiannya semakin menurun sehingga mencapai tahap terendahnya pada jam 12 selepas makan. Selepas itu, aktiviti pepsin meningkat semula dan terdapat rangsangan kecil dalam rembesan enzim yang dikesan pada 24 jam selepas makan. Aktiviti pepsin kemudiannya menurun semula kepada tahap sebelum makan. Tindak balas yang cepat terhadap pengambilan makanan menunjukkan ikan badut mampu menghadapi keadaan pembekalan makanan yang tidak menentu dengan berkesan.

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Histochemical demonstration of hydrochloric acid in the gastric tubules of teleosts using an in vivo prussian blue technique

Cited by 23 publications

References 10 publications

The ultrastructure of the gastric glands and its relation to induced secretory activity of cod, Gadus morhua (Day)

The ultrastructure of the gastric glands and its relation to induced secretory activity of cod, Gadus morhua (Day)

Feeding and digestion in two cottid fishes, the freshwater Cottus gobio L. and the marine Enophrys bubalis (Euphrasen)

Gastric Emptying and the Enzymatic Activity in the Stomach of Amphiprion ocellaris Fed on Artificial Diet

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