Histochemical Evaluation of Placental Dipeptidyl Peptidase IV (CD26) in Pre‐eclampsia: Enzyme Activity in Villous Trophoblast Indicates an Enhanced Likelihood of Gestational Hypertensive Disorders

Neudeck, H.; Joncic, Marion; Schuster, Christof; Bisson, S.; Hildebrandt, R.; Oney, T; Stiemer, B.; Hopp, H. Esteban; Graf, R.

doi:10.1111/j.1600-0897.1997.tb00259.x

Cited by 5 publications

(2 citation statements)

References 17 publications

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“…We now confirmed DPPIV protein expression in both MEDIUM/LARGE STB‐EVs and SMALL STB‐EVs of normal pregnancies (Figure 1(d) upper band) using specific immunoblotting. Consistent with the literature, we detected DPPIV in the whole placental tissue, with a signal localized in the syncytiotrophoblast layer (Figure 1(e)), demonstrating that DPPIV was present at the site of STB‐EVs production [26,27].…”

Section: Resultssupporting

confidence: 90%

Placental extracellular vesicles express active dipeptidyl peptidase IV; levels are increased in gestational diabetes mellitus

Kandžija

Zhang

Motta-Mejia

et al. 2019

J of Extracellular Vesicle

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Gestational diabetes mellitus (GDM) is the most common metabolic disorder in pregnancy and is characterized by insulin resistance and decreased circulating glucagon-like peptide-1 (GLP-1). GDM resolves rapidly after delivery implicating the placenta in the disease. This study examines the biological functions that cause this pathology. The placenta releases syncytiotrophoblast-derived extracellular vesicles (STB-EVs) into the maternal circulation, which is enhanced in GDM. Dipeptidyl peptidase IV (DPPIV) is known to play a role in type 2 diabetes by breaking down GLP-1, which in turn regulates glucose-dependent insulin secretion. STB-EVs from control and GDM women were analysed. We show that normal human placenta releases DPPIV-positive STB-EVs and that they are higher in uterine than paired peripheral blood, confirming placental origin. DPPIV-bound STB-EVs from normal perfused placentae are dose dependently inhibited with vildagliptin. DPPIV-bound STB-EVs from perfused placentae are able to breakdown GLP-1 in vitro. STB-EVs from GDM perfused placentae show greater DPPIV activity. Importantly, DPPIV-bound STB-EVs increase eightfold in the circulation of women with GDM. This is the first report of STB-EVs carrying a biologically active molecule that has the potential to regulate maternal insulin secretion.

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Section: Resultssupporting

confidence: 90%

Placental extracellular vesicles express active dipeptidyl peptidase IV; levels are increased in gestational diabetes mellitus

Kandžija

Zhang

Motta-Mejia

et al. 2019

J of Extracellular Vesicle

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“…The aberrant up-regulations of OPG, KiSS-1, VCAM, or PDGF-AA expression in placentas of preeclamptic pregnancies might closely correlate with the pathogenesis of PE [19-22]. Several enzymes, such as villous trophoblast dypeptidyl peptidase IV, 11-β hydroxysteroid dehydrogenase, and mitogen-activated protein kinase, are also abnormally activated in PE [23-25]. Nevertheless, up to date, there have been no attempts reported to screen the regulatory factors involved in placenta of preeclamptic pregnancies by large-scale proteomic analysis.…”

Section: Introductionmentioning

confidence: 99%

Comparative Proteome Profile of Human Placenta from Normal and Preeclamptic Pregnancies

et al. 2013

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To better understand the molecular mechanisms involved in pathological development of placenta in preeclampsia, we used LC-MS/MS to construct a large-scale comparative proteome profile of human placentas from normal and preeclamptic pregnancies. A total of 2636 proteins were detected in human placentas, and 171 different proteins were definitively identified between control and preeclamptic placentas. Further bioinformatics analysis indicated that these differentially expressed proteins correlate with several specific cellular processes which occur during pathological changes of preeclamptic placenta. 6 proteins were randomly selected to verify their expression patterns with Western blotting. Of which, 3 proteins’ cellular localizations were validated with immunohistochemistry. Elucidation of how protein-expression changes coordinate the pathological development would provide researchers with a better understanding of the critical biological processes of preeclampsia and potential targets for therapeutic agents to regulate placenta function, and eventually benefit the treatment of preeclampsia.

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Purification and Characterization of a Complex from Placental Syncytiotrophoblast Microvillous Membranes which Inhibits the Proliferation of Human Umbilical Vein Endothelial Cells

Kertész

Hurst

Ward³

et al. 1999

Placenta

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Histochemical Evaluation of Placental Dipeptidyl Peptidase IV (CD26) in Pre‐eclampsia: Enzyme Activity in Villous Trophoblast Indicates an Enhanced Likelihood of Gestational Hypertensive Disorders

Abstract: This study demonstrates for the first time that increased villous trophoblastic DPP IV activity indicates an increased likelihood of the presence and of the severity of hypertensive disorders in pregnancy.

Cited by 5 publications

References 17 publications

Placental extracellular vesicles express active dipeptidyl peptidase IV; levels are increased in gestational diabetes mellitus

Placental extracellular vesicles express active dipeptidyl peptidase IV; levels are increased in gestational diabetes mellitus

Comparative Proteome Profile of Human Placenta from Normal and Preeclamptic Pregnancies

Purification and Characterization of a Complex from Placental Syncytiotrophoblast Microvillous Membranes which Inhibits the Proliferation of Human Umbilical Vein Endothelial Cells

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