Histochemical localisation of carboxylesterase activity in rat and mouse oral cavity mucosa

Robinson, Darren; Bogdanffy, Matthew S.; Reed, Celia J.

doi:10.1016/s0300-483x(02)00375-x

Cited by 11 publications

(7 citation statements)

References 34 publications

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“…36 Site-specific expression of xenobiotic metabolizing enzymes within the oral cavity has not been reported in cats or humans; however, a study by Robinson et al demonstrated that carboxylesterase activity in the oral cavity of mice varied, depending on location, with an apparent correlation between lesion distribution following exposure to a high concentrations of vinyl acetate (a carcinogen) and high carboxylesterase activity in different areas of the oral cavity. 26 Bertone et al demonstrated a 2-fold increased risk for the development of OSCC in cats exposed to environmental tobacco smoke. 2 Perhaps the expression of polymorphic and site-specific xenobiotic metabolizing enzymes in the lingual mucosa makes some cats more susceptible to carcinogenesis, thereby developing OSCC of the tongue at an earlier age.…”

Section: Discussionmentioning

confidence: 99%

Bone-Invasive Oral Squamous Cell Carcinoma in Cats

et al. 2010

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Feline oral squamous cell carcinoma (OSCC) is the most common oral tumor in cats. There is no effective treatment, and the average duration of survival after diagnosis is only 2 months. Feline OSCC is frequently associated with osteolysis; however, the mechanisms responsible are unknown. The objective of this study was to characterize the epidemiology and pathology of bone-invasive OSCC in cats and to determine the expression of select bone resorption agonists. In sum, 451 cases of feline OSCC were evaluated. There was no sex or breed predisposition, although there were more intact cats in the OSCC group compared to the control group. Gingiva was the most common site, followed by the sublingual region and tongue. Cats with lingual OSCC were younger (mean, 11.9 years) compared to cats with gingival OSCC (mean, 13.6 years). In addition to osteolysis, there was periosteal new bone formation, osseous metaplasia of tumor stroma, and direct apposition of OSCC to fragments of bone, suggestive of bone-binding behavior. Eighty-two cases were selected for immunohistochemical detection of parathyroid hormone-related protein (PTHrP). Specimens with osteolysis had increased PTHrP expression and nuclear localization, compared to OSCC without osteolysis. Thirty-eight biopsies of OSCC with osteolysis were evaluated for tumor necrosis factor α expression, and only 4 biopsies had such expression in a small proportion of tumor cells. Increased tumor expression of PTHrP and increased localization of PTHrP to the nucleus were associated with osteolysis and may play an important role in bone resorption and tumor invasion in cats with OSCC.

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Section: Discussionmentioning

confidence: 99%

Bone-Invasive Oral Squamous Cell Carcinoma in Cats

et al. 2010

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“…Some authors found no mPD-L2 mRNA in brain tissue by Northern blotting [8]. Given that the mPD-L2 molecule was reported to be distributed mainly in organs of the immune system, we suspected that the mPD-L2-positive staining in mouse brain and diverse epithelia might be due to cross-reactivity with proteins other than mPD-L2.…”

Section: Cross-reactivity Of Anti-mpd-l2 Pab With Antigen(s) Other Thmentioning

confidence: 85%

“…(ii) Decalcification. Adult mouse olfactory epithelium was prepared as described previously [8], with modifications. Briefly, after fixation, the head of the mouse was submersed in 10% EDTA until the bone became half-transparent and soft when tested by application of needle tracks.…”

Section: Tissue Preparation and Stainingmentioning

confidence: 99%

Cross-reactivity of anti-programmed death ligand 2 polyclonal antibody in mouse tissues

Zhao

Bian

et al. 2012

Sci. China Life Sci.

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The inhibitory co-receptor programmed death 1 (PD-1, encoded by pdcd1) and its two ligands PD-L1 and PD-L2 comprise an important immune inhibitory signaling pathway for defense against microbes and for self-tolerance. Unlike other members of the B7-CD28 family, expression of PD-L1 and PD-L2 is not limited to the immune system. In this study, we determined that a polyclonal antibody (pAb) (R&D Systems) against extracellular domains of mouse PD-L2 (mPD-L2) could recognize antigen(s) in diverse mouse tissues, including the anterior and intermediate pituitary gland, olfactory bulbs and olfactory epithelium, tongue epithelium, keratinized epithelial cells and skin and whisker hair follicles. These findings differed from previous reports of mPD-L2 localization. Reverse transcription PCR and Western blot analyses, however, were unable to detect any mPD-L2 transcripts or proteins of the 25-kD predicted molecular weight in RNA and protein extracts, respectively, from the above tissues, suggesting that the anti-mPD-L2 pAb cross-reacts with certain novel antigen(s). Developmental studies revealed that the earliest expression of mPD-L2-like antigen was in the olfactory epithelium at embryonic day 12.5 (E12.5). At E14.5, mPD-L2-like antigen was present in the skin, tongue and follicles of the skin and whiskers. The distribution patterns of mPD-L2-like antigen remained similar from E18.5 to adulthood. The results of bioinformatic analysis and other experiments suggested neural cell adhesion molecule and hemicentin-1 as candidate proteins with cross-reactivity to the anti-mPD-L2 pAb. These results demonstrate that care is required in interpreting staining patterns generated when anti-PD-L2 pAb is used to locate PD-L2-expressing cells in the central nervous system and epithelial tissues, such as the olfactory epithelium. In addition, this anti-PD-L2 pAb may be used as an alternative antibody for labeling the olfactory epithelium during embryonic development in mice.

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“…(2) 脱钙. 参照以往成年小鼠嗅上皮制备的方法 [8] 并作修改: 固定后, 动物头部浸没在 10% EDTA 中, 直至骨骼成半透明状, 用针刺时变得柔软为止. 然后将脱钙后的脑组织置于 20%蔗糖中直至组织沉底.…”

Section: 组织制备和染色unclassified

“…有研究通过 Northern 印迹发现脑组织中没有 mPD-L2 mRNA 的表达 [8] . 考虑到曾有报道指出 mPD-L2 分子主要分布在免疫系统的器官上, 本文怀疑 mPD-L2 在小鼠脑和多种上皮组织中的表达可能是由于该抗体和 mPD-L2 抗原以外的其他抗原交叉反应的结果.…”

Section: Mpd-l2 多克隆抗体除 Mpd-l2 抗原外和其他抗原存在交叉反应unclassified

程序性死亡配体2(pd-L2)多克隆抗体在小鼠组织中的交叉反应性研究

刘¹,

王²,

郭³

et al. 2013

Sci. Sin.-Vitae

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Histochemical localisation of carboxylesterase activity in rat and mouse oral cavity mucosa

Cited by 11 publications

References 34 publications

Bone-Invasive Oral Squamous Cell Carcinoma in Cats

Bone-Invasive Oral Squamous Cell Carcinoma in Cats

Cross-reactivity of anti-programmed death ligand 2 polyclonal antibody in mouse tissues

程序性死亡配体2(pd-L2)多克隆抗体在小鼠组织中的交叉反应性研究

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