Histochemical localization in Ruta graveolens cell cultures: elucidating the relationship between cellular differentiation and furanocoumarin production

Abstract

Cell cultures of Ruta graveolens L. were used as a model system to study the relationship between cellular organization and furanocoumarin production. Relative contributions of individual cells were traced using a combination of biochemical and localization techniques in three types of cell cultures: dispersed, aggregated, and organized. The proportion of relative furanocoumarins produced varied with the organization level in cultures. Productive population in dispersed cell culture was 10% which increased to 17% and to 35% in aggregated and organized cell cultures, respectively. Large cell clusters accumulating furanocoumarins were restricted to organized cell cultures. In these lines, sites for psoralen, bergapten, and xanthotoxin accumulation were spatially separated from each other, which has been reported for the first time. Variation in production was due to change in relative size of productive population in the three types of cultures studied. A model has been proposed for differential furanocoumarin producing ability of cells based on differentiation levels.

Phytochemical Composition and Antioxidant Potential of Ruta graveolens L. In Vitro Culture Lines

“…Three cell culture lines with varying degree of differentiation (dispersed cell line RC1, aggregated cell line RC3 and differentiated cell line RC6) [10] were selected for evaluating antioxidant potential. Shoot line RS2 [11] and genetically transformed clone Ia3 (Manuscript under revision by Acta Physiologiae Plantarum) were selected due to their lower doubling time and high furanocoumarin productivity.…”

Efecto Tóxico del Extracto Acuoso de Ruta graveolens del Norte de México sobre el Hígado de Rata Wistar

“…La rutina se vende en farmacias como constituyente medicinal contra la fragilidad capilar, vermífugo intestinal y como antí-doto para las toxinas de serpiente y escorpión (Freire et al, 2010), además se ha utilizado para la psoriasis leucodérmica, linfomas cutáneos, artritis reumática y contra el cáncer (Diwan & Malpathak, 2009). Las furanocumarinas se han empleado para tratar la leucoderma, vitíligo, linfomas cutáneos y como agente anti-HIV (Diwan & Malpathak, 2010).…”

Coumarin Compounds in Coronilla scorpioides Callus Cultures

“…Coumarin production by in vitro cultures has been extensively reported for different species known as coumarin producers [24,25] and it was seen that the amount of coumarin produced in unorganized cell cultures [26][27][28] was comparatively less than that obtained in organized cultures [29][30][31][32].…”