Phytochemical Composition and Antioxidant Potential of Ruta graveolens L. In Vitro Culture Lines

Abstract

Ruta graveolens L. is a medicinal plant used in traditional systems of medicine for treatment of psoriasis, vitiligo, leucoderma, and lymphomas with well-known anti-inflammatory and anticancer properties. Therefore antioxidant potential of R. graveolens (in planta and in vitro) was investigated. As antioxidants present in plant extracts are multifunctional, their activity and mechanism depends on the composition and conditions of the test system. Therefore, the total antioxidant capacity was evaluated using assays that detect different antioxidants: free radical scavenging (DPPH and ABTS), transition metal ion reduction (phosphomolybdenum assay), reducing power, and nitric oxide reduction. Content of furanocoumarin-bergapten in the extracts showed good corelation with free radical scavenging, transition metal reduction and reducing power, while total phenolic content showed good corelation with nitric oxide reduction potential. Antioxidant activity of in vitro cultures was significantly higher compared to in vivo plant material. The present study is the first report on comprehensive study of antioxidant activity of R. graveolens and its in vitro cultures.

Light-induced fluctuations in biomass accumulation, secondary metabolites production and antioxidant activity in cell suspension cultures of Artemisia absinthium L.

“…Additionally, several reports are available on phenolics related antioxidant activity in the in vitro cultures of different plants [45][46][47][48]. In conclusion, light-grown suspension cultures of A. absinthium L. are potential entities for enhanced accumulation of phenolics.…”

Antioxidative Effects of Phenolic Compounds of Mushroom Mycelia in Simulated Regions of the Human Colon, In Vitro Study

“…The total antioxidant activity of samples was measured using the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid, ABTS) radical cation and expressed as equivalents of ascorbic acid (mmol/mL) [Diwan et al, 2012;Vamanu et al, 2013]. The control was represented by the activity determined before administration of the food supplement/product.…”

Localisation of furanocoumarins in the tissues and on the surface of shoots of Heracleum sosnowskyi

“…When washed away from the plant surface by rainfall, coumarins can also act as inhibitors of seed germination (Zobel and Brown 1995). The compounds inhibit cell division D r a f t (Diwan et al 2012). In addition to their diverse activities, furanocoumarins have been shown to have anticancer properties (Luo et al 2011;Bronikowska et al 2012).…”