Histochemical localization of NADPH-diaphorase in neurons of the pheasant ileum

Schmidtová, K; Kočišová, M

doi:10.1078/0065-1281-00661

Cited by 3 publications

(1 citation statement)

References 9 publications

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“…Neuropathological verification of spinal cord ischemic injury is based on currently used cellular stainings, histochemic methods (10), immunohistochemic methods or silver impregnation procedures. The results of our laboratory suggest that neurons after ischemic injury and reperfusion are stained, in early stage after ischemia, by suppressive Nauta method (7).…”

Section: Introductionmentioning

confidence: 99%

Visualization of Protein Aggregation in Nerve Cells After Ischemia/Reperfusion by Ubiquitin Immunohistochemistry and Impregnative Nauta Method

Mechírová¹,

Domoráková²,

Feriková³

2004

Acta Med. (Hradec Kralove, Czech Repub.)

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Using ubiquitin immunohistochemistry and impregnative Nauta method we demonstrated that ubiquitin positivity and Nauta positivity in the neurons affected with ischemic injury in the lumbosacral spinal cord of rabbits and dogs may be of the same origin. Increased number of ubiquitin-positive aggregates was found in the cytoplasm of neurons in the intermediate zone and lamina IX of ventral horns of spinal cord in rabbits after 30 min of ischemia followed by 24 h lasting reperfusion. Nauta-positive, flocculent, intracytoplasmic, dark clusters appeared in the same localization in the canine lumbosacral spinal cord neurons after 30 min of ischemia and 24 h of reperfusion. Ubiquitin aggregates and Nauta-positive dark clusters in the injured spinal cord neurons could be the first light microscopic signs of slow neuronal death following spinal cord ischemia and reperfusion.

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Section: Introductionmentioning

confidence: 99%

Visualization of Protein Aggregation in Nerve Cells After Ischemia/Reperfusion by Ubiquitin Immunohistochemistry and Impregnative Nauta Method

Mechírová¹,

Domoráková²,

Feriková³

2004

Acta Med. (Hradec Kralove, Czech Repub.)

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Acute infection with an avirulent strain of Toxoplasma gondii causes decreasing and atrophy of nitrergic myenteric neurons of rats

et al. 2017

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Comparative study of the distribution of NOS‐positive neurons in pigeon intestine

Sun

Gao

2020

Anat Histol Embryol

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Nitric oxide (NO) plays an important role in regulating gut motility, mucosal barrier function and secretions in the enteric nervous system. Nicotinamide adenine dinucleotide phosphate diaphorase (NADPH‐d) staining has been used to identify nitrergic neurons of the enteric nervous system in different species. However, NADPH‐d staining lacks specificity because it also reflects the presence of enzymes other than nitric oxide synthase (NOS). Therefore, NOS immunohistochemistry techniques are needed to test for nitrergic neurons in the avian gut. In the present work, the morphology, density and size of NOS‐positive neurons in the duodenum, jejunum, ileum, caecum and rectum myenteric plexus of adult pigeons were investigated using NOS immunohistochemistry and whole‐mount preparations techniques. The density of NOS‐positive ganglion was highest in the ileum, similar to the caecum and rectum, and the lowest staining levels were observed in the duodenum. The staining intensity of NOS‐positive neurons in the duodenum, jejunum and ileum was dark, followed by the rectal regions, with weak staining in the caecum. These results suggested that NOS immunohistochemistry and whole‐mount preparation techniques provide an effective assessment method of the ganglia in the pigeon intestinal myenteric nerve plexus and are more accurate for cell counting compared with conventional sections.

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Histochemical localization of NADPH-diaphorase in neurons of the pheasant ileum

Cited by 3 publications

References 9 publications

Visualization of Protein Aggregation in Nerve Cells After Ischemia/Reperfusion by Ubiquitin Immunohistochemistry and Impregnative Nauta Method

Visualization of Protein Aggregation in Nerve Cells After Ischemia/Reperfusion by Ubiquitin Immunohistochemistry and Impregnative Nauta Method

Acute infection with an avirulent strain of Toxoplasma gondii causes decreasing and atrophy of nitrergic myenteric neurons of rats

Comparative study of the distribution of NOS‐positive neurons in pigeon intestine

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