Histochemical Studies of Leukocytes from an Inflammatory Exudate. Glycogen and Phosphorylase

Glycogen granules in resting and inflammatory rainbow trout phagocytes-an ultrastructural study

“…injection of casein or LPS (lipopolysaccharide), Scott & Cooper (1974) found that at 24 h post-injection, the amount of glycogen of peritoneal neutrophils was increased 7-fold, while, at the same time, blood neutrophils showed only a 1.6-fold increase in glycogen content. This observation is in accordance with those of Wulff (1962) and Robinson et al (1982), and with our finding that trout with peritoneal cavities inflamed by 2 consecutive i.p. injections of bacteria have blood neutrophils with glycogen granules in amounts similar to those of the non-injected fish.…”

Increase in RNA and Glycogen but Not in DNA in Developing Human Macrophages Proven by Integrating Microdensitometry

“…In earlier quantitative cytochemical studies, blood monocytes were shown to contain only a quarter as much glycogen as neutrophils [Gahrton and Yataganas, 1976]. An increasing glycogen content during development has been suggested by changes in PAS staining intensity of macrophages in skin window preparations over a 28-hour period of study [Wulff, 1962]. As glycogen is a storage form of carbohy drate.…”

Histochemical Demonstration of Uridine Diphosphate Glucose-Glycogen Glucosyltransferase and Phosphorylase in Rabbit Blood and Bone Marrow Cells on Smears and Their Distribution

“…Thereby, he had performed a differentiation between polysaccharide synthesized by the enzyme activity and native glycogen. Cytochemical demonstration of UDPGGT in leucocytes of inflammatory exudate was reported by Wulff (40). Takeuchi and Sano (33) reported distribution of UDPGGT in blood and bone marrow cells using fixed and unfixed smears.…”

“…Eranko and Palkama (8) reported that the phosphorylase reaction became intense by adding polyvinyl pyrrolidone in the incubating medium. A further modification of method for applying in hematology was reported by Wulff and Sorensen (39,41,42). Their method permited the use of unfixed air-dried blood film, due to the protective effect of a high concentration of polyvinyl pyrrolidone in the incubating medium.…”