Histochemical Study of Lectin Binding to Gestational Endometrium

Bychkov, Vladimir; Toto, Patrick D.

doi:10.1097/00004347-198703000-00008

Cited by 9 publications

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“…The majority of lectin studies with human endometrium have employed light microscopy (LM) and have concentrated on glandular and stromal compartments (Aoki et al, 1989;Bychkov and Toto, 1987;Soderstrom, 1987;Wu et al, 1993) rather than the apical uterine epithelial surface. Furthermore, the limited evidence available on the apical uterine epithelial surface in primates is conflicting mostly because of the different methods used in the LM studies.…”

Section: Discussionmentioning

confidence: 99%

“…In general, the literature on Con A suggests that a weak homogeneous staining occurs across the uterine surface, irrespective of the physiological state of the epithelium or type of probes applied (Aoki et al, 1989;Bychkov and Toto, 1987;Duncan et al, 1988;Klentzeris et al, 1991;Kupryjanczyk, 1989). Conversely, the reported decrease in the density of Con A staining across the surface of ''infertile'' versus ''normal fertile'' women (Klentzeris et al, 1991) supports the present findings that indicate a likely role for ␣-mannosyl and ␣-glucosyl residues in preparing a receptive epithelium for blastocyst attachment.…”

Section: Discussionmentioning

confidence: 99%

“…In the few studies using endometrial samples from women (Aoki et al, 1989;Bychkov and Toto, 1987;Duncan et al, 1988;Kupryjanczyk, 1989), apes (Jones et al, 1998) and monkeys (Anderson et al, 1990) insufficient quantities of surface epithelium from curettings were generally obtained. The range of carbohydrates that typically constitute a plasma membrane glycocalyx has largely been unexplored in primate studies.…”

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Ultrastructural studies of glycan changes in the apical surface of the uterine epithelium during pre-ovulatory and pre-implantation stages in the marmoset monkey

1999

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It has been postulated that carbohydrates are involved in a variety of cell-cell interactions including blastocyst implantation. In primates, there are only limited investigations on the ultrastructural localisation of the cyclic changes in uterine epithelial surface carbohydrates. Our aim was to investigate such changes during the pre-ovulatory and pre-implantation stages of the reproductive cycle in the marmoset monkey.After fixation of endometrial tissues, avidin-ferritin lectin cytochemistry was employed for apical surface glycan detection at the ultrastructural level. Five lectins were used including Canavalia ensiformis (Con A), Lotus tetragonolobus (LTA), Glycine max (SBA), Phytolacca americana (PWM) and Triticum vulgaris (WGA). Morphometry was used to quantitate changes in the intensity of lectin staining by determining the total number of ferritin particles per unit length of membrane. Surface and intra-cytoplasmic vesicles, stained by the lectins, were also examined.Quantitative ferritin assessment showed that 1 day before presumed implantation (days 11 to 12 after ovulation in the marmoset monkey) there was a significant increase in Con A, LTA and SBA staining on the apical uterine epithelial plasma membrane compared to the pre-ovulatory phase and earlier stages of pregnancy (days 4-8 after ovulation). A significant increase in PWM was also detected from early pregnancy to preimplantation stages. All lectins except WGA produced reproducible staining within reproductive cycle groups. The greatest variation and intensity of epithelial surface staining was observed with WGA and the weakest with LTA. The patchy staining with LTA compared with thick coverage by WGA indicated the complexity of the carbohydrate arrangement in the glycocalyx of the uterine surface plasma membrane. Reduction of WGA reactivity after neuraminidase treatment suggested that the lectin binding might be related to the presence of heavily sialylated apical uterine membrane glycoconjugates.This is the first high-resolution study in primates to report quantitative cyclic changes in fucosyl, galactosyl, glucosyl, and mannosyl sugar residues of the apical uterine epithelial glycocalyx. The findings support the concept Abbreviations: DC, NЈN diacetyl chitobiose; Fuc, fucose; Glc, glucose; Man, mannose; GalNAc, N-acetyl galactosamine; GlcNAc, N-acetyl glucosamine; NeuNAc, neuraminic acid (sialyl residues); NЈdase, neuraminidase; sWGA, succinyl wheatgerm; WGA, native wheatgerm; nil, not applied; 0 to 7, lectin staining on a scale from 0 (negative) to 7 (heavy).

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Ultrastructural studies of glycan changes in the apical surface of the uterine epithelium during pre-ovulatory and pre-implantation stages in the marmoset monkey

1999

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“…Application of lectin histochemistry for the study of ovarian neoplasms is very limited [15,16], though the results already received (including our preliminary data [17,18]) indicate the usefulness of the method for the better understanding of ovarian tumors and their malig nant potential.…”

Section: Introductionmentioning

confidence: 92%

“…Such organs as the ovary and the uterus seem to be especially suitable for this type of histochemical analysis by virtue of their fast pace of altera tions in the course of the normal menstrual cycle and in the development of neoplasia. Though the number of publications on lectin binding in gynecologic pathology is small and mostly confined to the study of uterine cervix [11], and endometrium [12][13][14], the results of these stud ies demonstrate the utility of this method.…”

Section: Introductionmentioning

confidence: 97%

Lectin Binding to Common Epithelial Tumors of the Ovaries

Bychkov¹,

Dolan²,

Reddy³

1991

Gynecol Obstet Invest

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Binding sites of peanut agglutinin (PNA), Ulex europaeus (UEA-1), wheat germ agglutinin, concanavalin A, and Ricinus communis were localized in 46 common epithelial ovarian tumors. Binding pattern of serous cystad-enomas showed significant similarity with the pattern seen in the epithelium of fallopian tubes. Lectin affinity of endometriosis and endometrioid tumors was similar to normal endometrium in secretory phase. Binding pattern of clear cell carcinomas was similar to gestational endometrium, with PNA and UEA-1 showing strong affinity. This pattern is in contrast to clear cell carcinomas of the kidney, that do not bind PNA and UEA-1. Mucinous cystade-nomas exhibited binding patterns identical with cervical epithelium. Malignant counterparts of the described tumors were characterized by the partial loss of lectin binding and uneven distribution of the binding reaction.

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Cycle- and function-related changes in lectin binding to human endometrium: a histochemical study with pronase treatment

Kupryjańczyk

1989

Arch Gynecol Obstet

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Forty-eight endometrial biopsy specimens were obtained during a normal menstrual cycle, during pregnancy and from patients with dysfunctional bleeding. The specimens were examined for Peanut (PNA), Soybean (SBA), Vicia villosa (VVA), Phytohem- (PHA), Lens culinaris (LCA) and Concanavalin (succ. Con A) agglutinin binding. The study was performed on paraffin sections using the pronase digestion and either the peroxidase-antiperoxidase or the avidin-biotin-peroxidase method. Cycle-related changes of the PNA, SBA, VVA, and to some degree PHA binding, were based on the transfer of the cytoplasmic reaction toward the glandular lumena. PNA + and SBA + material moved to the cell surface at the transition of the follicular and luteal phase and before the basal vacuolization appeared. Functional disturbances mainly influenced the intensity of the reaction. It was true only for those lectins, whose binding pattern showed cycle-related changes. In curettings from patients with a prolonged menstrual cycle the lectin binding seen in normal late secretory endometrium was absent or significantly diminished. Lectin binding to the endometrial surface epithelium was variable; PHA was the only lectin, the binding pattern of which followed cyclic changes in the glycocalyx, detected previously by means of PAS and alcian blue methods.

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Histochemical Study of Lectin Binding to Gestational Endometrium

Cited by 9 publications

References 0 publications

Ultrastructural studies of glycan changes in the apical surface of the uterine epithelium during pre-ovulatory and pre-implantation stages in the marmoset monkey

Ultrastructural studies of glycan changes in the apical surface of the uterine epithelium during pre-ovulatory and pre-implantation stages in the marmoset monkey

Lectin Binding to Common Epithelial Tumors of the Ovaries

Cycle- and function-related changes in lectin binding to human endometrium: a histochemical study with pronase treatment

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