Histochemistry of Leukocytes

Wachstein, M.

doi:10.1111/j.1749-6632.1955.tb46001.x

Cited by 41 publications

(8 citation statements)

References 40 publications

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“…The observations on the developing control kidney in the present investigation are consistent with those of Les son [1960] and Larson [1975], The localization of succinic dehydrogenase conforms with that of Wachstein and Bradshaw [1955].…”

Section: Discussionsupporting

confidence: 81%

Experimental Study on Developing Rat Kidney after <i>Naja haje</i> Envenomation

Hessy¹,

Rahman²,

Iskander³

et al. 1987

Cells Tissues Organs

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In the present experiment 164 pregnant white Wistar rats were used to study the effect of Naja haje (Egyptian cobra) venom on the developing kidney. The rats were divided into 3 groups; a control group, a group receiving one LD₅₀ of N. haje venom and the third injected with % of LD₅₀. The injection was performed at different stages of gestation. After birth, the neonates of group I and III and embryos of group II were examined histologically, histochemically and electron-microscopically. Both lethal and sublethal doses of N. haje venom produced haemorrhages and vascular congestion of the developing kidney. The lethal dose had degenerative effects on the podocytes and endothelium. Tubular damage appeared mainly as mitochondrial degeneration and bud-like extension, protrusions of cytoplasm and vacuolization. The succinic dehydrogenase enzyme showed decreased activity. The sublethal dose had an effect on the glomerular basement membrane in the form of splitting, increased mesangial cells and matrix, mitochondrial degeneration and fusion of podocyte processes. Tubulization of the parietal epithelium, vacuolization of the proximal tubules, mitochondrial degeneration and apical budding were evident.

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Section: Discussionsupporting

confidence: 81%

Experimental Study on Developing Rat Kidney after <i>Naja haje</i> Envenomation

Hessy¹,

Rahman²,

Iskander³

et al. 1987

Cells Tissues Organs

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“…It is improbable that the accumulation of glycogen re presents a degenerative phenomenon. Firstly cells showing morpho logical signs of degeneration were not frequent in the preparations, and secondly blood neutrophils may stain equally intensely for instance in cases of acute infections (11).…”

Section: Commentmentioning

confidence: 99%

Histochemical Studies of Leukocytes from an Inflammatory Exudate. Glycogen and Phosphorylase

Wulff¹

1962

Acta Haematol

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“…Few histochemical studies have been reported concerning the localization of dehydrogenase activity in the cells of the blood and bone marrow (1,8,14,15,17). Wachstein's studies (14,15) indicated the presence of dehydrogenase activity in many of the cellular elements of normal and abnormal blood, bone marrow, and lymphatic tissue, as well as in exudates, by the use of a buffered neotetrazolium (2,2'-(p-diphenylene)bis(3,5-diphenyl) tetrazolium chloride) incubating medium. These studies indicated further that the reaction was improved by the addition of sodium succinate or various activators to the incubating solution.…”

mentioning

confidence: 99%

Histochemical Demonstration of Dehydrogenase Activity in the Cells of Normal Human Blood and Bone Marrow

Ackerman

1960

The Journal of Cell Biology

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Endogenous and succinic dehydrogenase activity was demonstrated in the living cells of normal human blood and bone marrow using a buffered nitro BT-succinate incubating solution. With this technique dchydrogenase activity was localized primarily in the granular leukocytes and the sites of cnzymatic activity appcared to be non-mitochondrial. The addition of a non-ionic surface active agent to thc incubating solution resulted in marked differences in the cellular and intracellular localization of dehydrogenase activity. With this method it was possible to demonstrate dehydrogenase activity in the mitochondria of most of the formed elements of the blood and bone marrow, including developing granulocytes and erythroid cells, agranulocytes, and blood platelets. Mature erythrocytes also exhibited a minimal dehydrogenase reaction with this procedure. This investigation indicated that in order adequately to demonstrate and evaluate dehydrogenase activity in the cells of the blood and bone marrow it was necessary to have increased cellular and mitochondrial permeability, as well as partially viable cells with an intact dehydrogenase system.

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Histochemistry of Leukocytes

Cited by 41 publications

References 40 publications

Experimental Study on Developing Rat Kidney after <i>Naja haje</i> Envenomation

Experimental Study on Developing Rat Kidney after <i>Naja haje</i> Envenomation

Histochemical Studies of Leukocytes from an Inflammatory Exudate. Glycogen and Phosphorylase

Histochemical Demonstration of Dehydrogenase Activity in the Cells of Normal Human Blood and Bone Marrow

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