Histogenesis of Preneoplastic and Neoplastic Lesions in Tracheobronchial Epithelium

Biosynthesis and native granule characteristics of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in Delftia acidovorans

“…Harvested cells were prefixed in the presence of McDowellTrump fixative [17] in 0.1 M phosphate buffer (pH 7.2) and then washed in the same buffer for 30 min. Cells were then post-fixed in 1% osmium tetroxide at room temperature for approximately 2 h. Post-fixed cells were washed twice with distilled water and later dehydrated with a graded ethanol series.…”

Airway mucosa: secretory cells, mucus and mucin genes

“…MCDOWELL and TRUMP [35] have presented evidence and argued convincingly that such an epidermoid change arises subsequent to division of mucous cells, rather than arising directly from existing basal cells. Experimentally, carcinogens, mechanical trauma and vitamin A deficiency may each induce changes in mucous cells, leading to squamous cell metaplasia with or without stratification and keratinization [36].…”

Stem cells of the respiratory epithelium and their in vitro cultivation

“…Occasionally, there are small periodic acid Schiff (PAS)-positive granules. This first stage may be regarded as a predifferentiation stage, at which many of the uhrastrnctural features resemble those of the small mucus granule cells observed in a regenerating hamster and human airway epithelium (McDowell and Trump, 1983). We detected antigenicity of Clara cell-specific 10 kDa protein (CC10) (Singh et al, 1988) in about 30% of the cells at this first stage with most of the positive reaction in the cytoplasm being rather homogeneous (agranular).…”

“…Under the conventional culture conditions, the cells are phenotypically undifferentiated with poorly developed smooth and rough endoplasmic reticulum (sER and rER, respectively) and Golgi complex, and no secretory granules (Emura et at., 1982). They do not possess features of basal cells (Inayama et al, 1989) but rather resemble the intermediate cells described by McDowell and Trump (1983) or the undifferentiated nonciliated bronchiolar epithelial (Clara) cells described by Plopper et at. (1992).…”

“…for Clara ceils and type II cells (Stripp et al, 1995) Undifferentiated "indifferent" cells produced by dedifferentiation of differentiated secretory, basal, and ciliated cells, and SMGC for goblet and secretory cells (McDowell et al, 1982) Intermediate ceils as a pluripoteut precursor for all cell types (McDowell and Trump, 1983) Stem cells for all cell types (McDowell, 1987;Marchevsky, 1990) Clara cells only for themselves and ciliated but not for basal and mucous cells, rejecting possibility of dedifferentiation (Brody et al, 1987) Basal cells for themselves, goblet and ciliated cells and SMGC (Inayama et al, 1988(Inayama et al, , 1989Nettesheim et al, 1990) a Basal cells for themselves, ciliated and mucous cells (Moller et al, 1989) a,b Secretory cells, more pluripotent than basal cells (Johnson and Hubbs, 1990) a Basal cells, pluripotent (Ford and Terzaghi-Howe, 1992) a Pluripotent undifferentiated cells produced by dedifferentiation of differentiated secretory and basal cells (Liu et al, 1994) Pluripoteut stem ceils (Engelhardt et al, 1995) '~The isolated epithelial cell fractions may have contained a small population of indiscrete undifferentiated epithelial cells (possibly pluripotent stem cells). bplated on a collagen-coated porous membrane.…”