Histologic and skeletal abnormalities in benzo(a)pyrene-treated rainbow trout alevins

Hose, Jo Ellen; Hannaht, James B.; Puffer, Harold W.; Landolt, Marsha L.

doi:10.1007/bf01055930

Cited by 47 publications

(15 citation statements)

References 27 publications

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“…Notwithstanding the fact that in fish, lower MN frequencies, both spontaneous and induced, can be observed with respect to mammals (Rizzoni et al, 1987), a dose-dependent and time-dependent response has been ascertained by many authors (Hooftman and Vink, 1981;Hose et al, 1984;Das and Nanda, 1986). This confirms the suitability of this test system to study the relationship between pollution levels and mutagenic toxicity.…”

Section: Discussionsupporting

confidence: 62%

Micronucleus test in erythrocytes of Barbus plebejus (Teleostei, Pisces) from two natural environments: a bioassay for the in situ detection of mutagens in freshwater

Minissi

Ciccotti

Rizzoni

1996

Mutation Research/Genetic Toxicology

132

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Erythrocyte micronucleus frequencies in wild fish from two riverine environments and in fish reproduced and reared under controlled conditions (control group) were compared, with the aim to evaluate the suitability of the MN test for the in situ detection of mutagens in freshwaters. Fish were caught in different months in two rivers of central Italy which have different pollution levels. As indicator species, the barbel (Barbus plebejus) was chosen because of its ecological significance. Blood samplings were performed on wild fish immediately after capture and repeated at different time intervals on the same individuals, which were maintained in controlled conditions after capture. A total of 10000 erythrocytes per specimen were scored. No significant differences in micronucleus frequencies were observed between the control group and fish from the unpolluted river (Mignone). A significantly higher frequency of micronuclei was observed in fish caught in the polluted river (Tiber), in comparison to both the controls and the Mignone river fish. No significant seasonal differences were observed. Barbels examined 50 and 100 days after capture presented a remarkable decrease in micronucleus frequency in comparison with the frequency observed in barbels at capture. The micronucleus test in fish erythrocytes was shown to be a sensitive bioassay for detecting mutagenic pollution in fresh water environments.

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Section: Discussionsupporting

confidence: 62%

Micronucleus test in erythrocytes of Barbus plebejus (Teleostei, Pisces) from two natural environments: a bioassay for the in situ detection of mutagens in freshwater

Minissi

Ciccotti

Rizzoni

1996

Mutation Research/Genetic Toxicology

132

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“…23,42 In the current study, we demonstrated that subchronic exposure of adult medaka fish to BaP caused similar skeletal deformities (scoliosis) in the F1 generation eleutheroembryos ( Figure 3D). The spines of most skeletal deformational eleutheroembryos were curved from side to side, resembling a "C" or "S" rather than a straight line.…”

Section: ■ Discussionmentioning

confidence: 75%

Modulation of Benzo[a]pyrene-Induced Toxic Effects in Japanese Medaka (Oryzias latipes) by 2,2′,4,4′-Tetrabromodiphenyl Ether

Zhao

Luo

Fan

et al. 2013

Environ. Sci. Technol.

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Because polycyclic aromatic hydrocarbons (PAHs) and polybrominated diphenylethers (PBDEs) are ubiquitous and coexist in the environment and in wildlife, there are potential interactions between them that could cause toxic effects. In this study, the modulating effects of 2,2',4,4'-tetrabromodiphenyl ether (BDE47) and benzo[a]pyrene (BaP)-induced reproductive and developmental toxic effects in Japanese medaka (Oryzias latipes) were investigated by exposing adult Japanese medaka to BaP alone, BDE47 alone, and coexposing them with both BaP and BDE47 at different concentrations, respectively. Exposure to BaP alone significantly suppressed fecundity and egg protein content and markedly induced skeletal deformation in F1 generation eleutheroembryos. BDE47 significantly recovered reproductive functions, fecundity, and egg protein content, suppressed by BaP when the concentration of BDE47 increased to 0.44 μg/L. Such effects can be at least partly explained by the decreased BaP levels in the coexposure groups and the accompanying increase in the circulating level of 17β-estradiol in female medaka fish. The prevalence of skeletal deformations markedly increased to 19.3 ± 2.4% and 16.0 ± 1.6% in fish coexposed to BaP and BDE47 at 0.44 and 2.58 μg/L compared with that of fish exposed to 1.21 μg/L BaP alone (9.7 ± 1.7%), and the impacts on male medaka fish in the coexposure groups would be the crucial reason leading to these effects. Considering that the measured water concentrations of BaP and BDE47 in the present study were comparable with those reported in rivers and harbors, BaP and BDE47 contamination in the real world would have a significant level of interactive effects on wild fish.

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“…Maternal exposure to PAHs can also result in reduced survival of eggs and fry with survivors exhibiting physical malformations (Hose et al, 1984;Hall & Oris, 1991). Embryos and larvae of shortnose sturgeon Acipenser brevirostrum (Lesueur) exposed to coal tar-contaminated sediments rich on PAHs produced over 95% embryo-larval mortality (Kocan et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Effects of sediment eluates and extracts from differently polluted small rivers on zebrafish embryos and larvae

Strmac

Oberemm

Braunbeck

2002

Journal of Fish Biology

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The effects on newly fertilized eggs, embryos and larvae of zebrafish Danio rerio following exposure to sediment samples from the more heavily contaminated River Kö rsch, southern Germany, occurred earlier and were more prominent than in samples from the less contaminated Krähenbach. Dose-and time-related effects following exposure to Kö rsch sediment eluates and extracts included: (1) hatching failure and subsequent death of larvae exposed to undiluted aqueous sediment eluates and reduced hatching rates at sediment extract concentrations d0·0125%; (2) increased mortality after exposure to 25 and 50% dilutions of aqueous sediment eluates, and dilutions of d0·00625% sediment extracts; (3) reduction of heart beat frequency for 50% dilutions of sediment eluates and concentrations of d0·025% extracts; (4) increased frequency of heart and yolk sac oedema after exposure to d0·0125% sediment extracts. Since adverse effects of sediment extracts observed in zebrafish laboratory tests correlated with reproductive failure in natural populations of brown trout Salmo trutta f. fario in the severely polluted River Kö rsch, early life stages tests with zebrafish appear to be a suitable tool to assess the contamination rate of natural sediments.

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Histologic and skeletal abnormalities in benzo(a)pyrene-treated rainbow trout alevins

Cited by 47 publications

References 27 publications

Micronucleus test in erythrocytes of Barbus plebejus (Teleostei, Pisces) from two natural environments: a bioassay for the in situ detection of mutagens in freshwater

Micronucleus test in erythrocytes of Barbus plebejus (Teleostei, Pisces) from two natural environments: a bioassay for the in situ detection of mutagens in freshwater

Modulation of Benzo[a]pyrene-Induced Toxic Effects in Japanese Medaka (Oryzias latipes) by 2,2′,4,4′-Tetrabromodiphenyl Ether

Effects of sediment eluates and extracts from differently polluted small rivers on zebrafish embryos and larvae

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