Histologic Evaluation of Bone Regeneration using Titanium Mesh Prepared by Selective Laser Melting Technique

Yamamoto, Kayoko; Yamaguchi, Seiji; Matsushita, Tomiharu; Mori, Shigeo; Kitagaki, Hisashi; Yoshimura, Hitoshi; Sano, Kazuo; Sunano, Akihiro; Nakajima, Yoshiyuki; Nakano, Hirofumi; Ueno, Takaaki

doi:10.2485/jhtb.26.257

Cited by 6 publications

(5 citation statements)

References 14 publications

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“…Thus, we previously evaluated the osteogenic activity in a study in which a rat calvarial bone defect was covered with the Ti mesh subjected to the MAH treatment, as well as other surface treatments. The results showed that the MAH-treated Ti mesh exhibited the highest bone formation in the bone defect area from an early stage, demonstrating the effectiveness of the MAH treatment on the Ti mesh [ 30 , 31 , 32 ].…”

Section: Discussionmentioning

confidence: 99%

Mechanical, Histological, and Scanning Electron Microscopy Study of the Effect of Mixed-Acid and Heat Treatment on Additive-Manufactured Titanium Plates on Bonding to the Bone Surface

et al. 2020

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The additive manufacturing (AM) technique has attracted attention as one of the fully customizable medical material technologies. In addition, the development of new surface treatments has been investigated to improve the osteogenic ability of the AM titanium (Ti) plate. The purpose of this study was to evaluate the osteogenic activity of the AM Ti with mixed-acid and heat (MAH) treatment. Fully customized AM Ti plates were created with a curvature suitable for rat calvarial bone, and they were examined in a group implanted with the MAH-treated Ti in comparison with the untreated (UN) group. The AM Ti plates were fixed to the surface of rat calvarial bone, followed by extraction of the calvarial bone 1, 4, 8, and 12 weeks after implantation. The bonding between the bone and Ti was evaluated mechanically. In addition, AM Ti plates removed from the bone were examined histologically by electron microscopy and Villanueva–Goldner stain. The mechanical evaluation showed significantly stronger bone-bonding in the MAH group than in the UN group. In addition, active bone formation was seen histologically in the MAH group. Therefore, these findings indicate that MAH resulted in rapid and strong bonding between cortical bone and Ti.

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Section: Discussionmentioning

confidence: 99%

Mechanical, Histological, and Scanning Electron Microscopy Study of the Effect of Mixed-Acid and Heat Treatment on Additive-Manufactured Titanium Plates on Bonding to the Bone Surface

et al. 2020

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“…A surface roughness was produced on the Ti metal sample by the acid treatment, and this remained unchanged up to 600 • C, but the sample surface roughness decreased above 700 • C. Yamaguchi dipped mixed-acid and heat-treated Ti into simulated body fluid and then assessed its effect on bone formation [18]. In addition, Yamamoto et al [19,20] created a small bone defect in rat calvarial bones and implanted Ti mesh that had been subjected to various surface treatments into the defect. They found significantly greater bone formation with Ti specimens that had been subjected to mixed acid and heat treatment.…”

Section: Discussionmentioning

confidence: 99%

Histological Evaluation of Porous Additive-Manufacturing Titanium Artificial Bone in Rat Calvarial Bone Defects

et al. 2021

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Jaw reconstruction using an additive-manufacturing titanium artificial bone (AMTAB) has recently attracted considerable attention. The synthesis of a titanium artificial bone is based on three-dimensional computed tomography images acquired before surgery. A histological evaluation of porous AMTAB (pAMTAB) embedded in rat calvarial bone defects was conducted. This study examined three groups: rats implanted with mixed-acid and heat-treated pAMTAB, rats implanted with untreated pAMTAB, and rats with no implant. In both pAMTAB groups, bone defects were created in rat calvarial bones using a 5-mm trephine bar, followed by pAMTAB implantation. The pAMTAB was fixed to the defect using the fitting force of the surrounding bones. The rats were sacrificed at 4, 8, and 16 weeks after implantation, and the skull was dissected. Undecalcified ground slides were prepared and stained with Villanueva Goldner. Compared with the no implant control group, both pAMTAB groups exhibited new bone formation inside the defect, with greater bone formation in the mixed-acid and heat-treated pAMTAB group than in the untreated pAMTAB group, but the difference was not significant. These data suggest that pAMTAB induces bone formation after implantation in bone defects. Bone formation appears to be enhanced by prior mixed-acid and heat-treated pAMTAB.

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“… 23 The fact that Ti mesh prepared by SLM possesses osteogenic capacity is not inconsistent with our previous report. 24 …”

Section: Discussionmentioning

confidence: 99%

Osteogenic capacity of mixed-acid and heat-treated titanium mesh prepared by a selective laser melting technique

et al. 2018

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The practical use of additive manufacturing to create artificial bone as a material for repairing complex bone defects is currently attracting attention. In this study, we compared the osteogenic capacity of materials composited by the method developed by Kokubo et al. of treating 3D-printed titanium (Ti) mesh with a mixture of H 2 SO 4 and HCl and heating (mixed-acid and heat treatment) with that of materials subjected to conventional chemical treatment. Ti plates treated with this method have been found to promote highly active bone formation on their surface when inserted into rabbit tibial bone defects. No previous study has compared this method with other surface treatment methods. In this study, we used histological and other observations to compare the bone formation process in bone defects when Ti meshes prepared by the selective laser melting technique (SLM) and treated either with mixed acids and heat or with conventional chemical Ti surface treatments were implanted in a rat calvarial bone defect model. We found that both micro-computed tomography and observations of undecalcified ground sections showed that the best bone formation was observed in rats implanted with mesh treated with mixed acids and heat. Our results suggest that mixed-acid and heat-treated Ti mesh prepared by SLM may have a high osteogenic capacity in bone defects.

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Histologic Evaluation of Bone Regeneration using Titanium Mesh Prepared by Selective Laser Melting Technique

Cited by 6 publications

References 14 publications

Mechanical, Histological, and Scanning Electron Microscopy Study of the Effect of Mixed-Acid and Heat Treatment on Additive-Manufactured Titanium Plates on Bonding to the Bone Surface

Mechanical, Histological, and Scanning Electron Microscopy Study of the Effect of Mixed-Acid and Heat Treatment on Additive-Manufactured Titanium Plates on Bonding to the Bone Surface

Histological Evaluation of Porous Additive-Manufacturing Titanium Artificial Bone in Rat Calvarial Bone Defects

Osteogenic capacity of mixed-acid and heat-treated titanium mesh prepared by a selective laser melting technique

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