Histologic evaluation of skin‐derived and collagen‐based substrates implanted in palatal wounds

Ophof, Ricardo; Maltha, Jaap C.; Hoff, Johannes W. Von den; Kuijpers-Jagtman, Anne Marie

doi:10.1111/j.1067-1927.2004.012505.x

Cited by 40 publications

(29 citation statements)

References 54 publications

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“…We only found a transient influx of myofibroblasts between 1 and 2 weeks, including those of the sham groups, which is in accordance with a study on collagen scaffolds implanted in the palate of dogs. 47 Myofibroblasts are related to both wound contraction and scar formation. 48,49 In palatal wounds of rats, myofibroblasts are mainly present between 4 and 22 days, with a peak intensity after 1 week.…”

Section: Discussionmentioning

confidence: 99%

Collagen Scaffolds Implanted in the Palatal Mucosa

Jansen

Kuijpers-Jagtman²,

Kuppevelt³

et al. 2008

Journal of Craniofacial Surgery

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Scar formation after repair of the cleft palate leads to growth impairment of the upper jaw and midface. The implantation of a suitable scaffold during surgery may reduce this adverse effect. However, little is known about tissue reactions to scaffolds implanted in the oral cavity. Our goal was to analyze the tissue reactions to cross-linked type I collagen scaffolds after submucoperiosteal implantation in the palate of rats. Collagen type I scaffolds were implanted in the palate of 25 male Wistar rats. Groups of 5 rats were killed consecutively after 1, 2, 4, 8, and 16 weeks and were processed for histologic and immunohistochemical analyses. After 1 and 2 weeks, 3 rats from the sham group were also killed. On hematoxylin and eosin-stained sections, the cell density and the number of giant cells were determined. Blood vessels, inflammation, and the presence of myofibroblasts were detected by immunohistochemistry. An influx of inflammatory cells started after 1 week but had completely subsided after 8 weeks. Myofibroblasts were observed within the scaffolds only in the first 2 weeks. Angiogenesis already started after 1 week and showed a peak after 4 weeks, slowly declining afterward. The scaffolds were gradually integrated within the host tissue and only elicited a mild and transient inflammatory response. The scaffolds were biocompatible and seemed to be promising for future applications in cleft palate surgery.

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Section: Discussionmentioning

confidence: 99%

Collagen Scaffolds Implanted in the Palatal Mucosa

Jansen

Kuijpers-Jagtman²,

Kuppevelt³

et al. 2008

Journal of Craniofacial Surgery

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“…Ophof et al reported fewer histologic indications of scar tissue formation in open palatal wounds in beagle dogs grafted with various dermal substrates. 42 Reducing scar formation and wound contracture may help to decrease the incidence of oronasal fistulas and to reduce palatal shortening and its associated functional deficits. In 2003, Clark et al 43 reported their experience with human acellular dermal matrices in treating six cleft palate patients.…”

Section: Discussionmentioning

confidence: 99%

Repair of Oronasal Fistulae with Acellular Dermal Matrices

Kirschner

Cabiling

Slemp

et al. 2006

Plastic and Reconstructive Surgery

105

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“…Thus, picrosirius stained histological slices have been examined under bright field illumination. The quantification of collagen in picrosirius stained slices using bright field has been described elsewhere [38]–[44]. For each animal, a mean value was derived from the analysis of 9 histological slices.…”

Section: Methodsmentioning

confidence: 99%

Administration of Bleomycin via the Oropharyngeal Aspiration Route Leads to Sustained Lung Fibrosis in Mice and Rats as Quantified by UTE-MRI and Histology

et al. 2013

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Pulmonary fibrosis can be experimentally induced in small rodents by bleomycin. The antibiotic is usually administered via the intratracheal or intranasal routes. In the present study, we investigated the oropharyngeal aspiration of bleomycin as an alternative route for the induction of lung fibrosis in rats and mice. The development of lung injury was followed in vivo by ultrashort echo time magnetic resonance imaging (UTE-MRI) and by post-mortem analyses (histology of collagen, hydroxyproline determination, and qRT-PCR). In C57BL/6 mice, oropharyngeal aspiration of bleomycin led to more prominent lung fibrosis as compared to intranasal administration. Consequently, the oropharyngeal aspiration route allowed a dose reduction of bleomycin and, therewith, a model refinement. Moreover, the distribution of collagen after oropharyngeal aspiration of bleomycin was more homogenous than after intranasal administration: for the oropharyngeal aspiration route, fibrotic areas appeared all over the lung lobes, while for the intranasal route fibrotic lesions appeared mainly around the largest superior airways. Thus, oropharyngeal aspiration of bleomycin induced morphological changes that were more comparable to the human disease than the intranasal administration route did. Oropharyngeal aspiration of bleomycin led to a homogeneous fibrotic injury also in rat lungs. The present data suggest oropharyngeal aspiration of bleomycin as a less invasive means to induce homogeneous and sustained fibrosis in the lungs of mice and rats.

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Histologic evaluation of skin‐derived and collagen‐based substrates implanted in palatal wounds

Cited by 40 publications

References 54 publications

Collagen Scaffolds Implanted in the Palatal Mucosa

Collagen Scaffolds Implanted in the Palatal Mucosa

Repair of Oronasal Fistulae with Acellular Dermal Matrices

Administration of Bleomycin via the Oropharyngeal Aspiration Route Leads to Sustained Lung Fibrosis in Mice and Rats as Quantified by UTE-MRI and Histology

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