Histologic observation, identification, and secondary metabolites analysis of endophytic fungi isolated from a medicinal plant, hedychium acuminatum Roscoe

Abstract

Abstract. Some endophytic fungi live in medicinal plant tissue and could produce some secondary metabolites. This research was done to 1) determine the endophytic fungi position in the Hedychium acuminatum Roscoe. plant tissue by histological observation, 2) identify the endophytic fungi species isolated from the rhizome, leaf sheath, and the leaf of H. acuminatum, and 3) analyze the contents of some secondary metabolites that be produced by the endophytic fungi species isolated from H. acuminatum. The determination of the position of endophytic fungi in the H. acuminatum plant tissue was done by histologic observation. The endophytic fungi were isolated from H. acuminatum plant, then were inoculated on Potato Dextrose Agar medium and incubated at 25 °C during 7 to 14 × 24 h. Each endophytic fungus isolate was identified. Each endophytic fungus isolate was inoculated in Potato Dextrose Broth medium and shook at 120 rpm for 7 × 24 h; then the secondary metabolites content was analyzed using the spectrophotometric method. The research result was: 1) the endophytic fungi mycelium were found on the parenchyma cell wall and intercellular space of rhizome, epidermis and parenchyma cell wall of leaf sheath, epidermis cell wall of leaf; 2) eleven endophytic fungi species were found: Rhizoctonia sp., Fusarium solani, F. oxysporum, Colletotrichum alienum, C. aotearoa, C. ti, Aspergillus parasiticus, C. coccodes, C. gloeosporoides, Hansfordia biophila, and F. semitectum; 3) the secondary metabolites content in fungi culture in broth medium are: alkaloid 20.48 μm · mL -1 to 117.12 μm · mL -1 ), flavonoid (148.48 μm · mL -1 to 816.96 μm · mL -1 ), terpenoid (4.73 μm · mL -1 to 32.98 μm · mL -1 ), and tannin (9.39 μm · mL -1 to 53.02 μm · mL -1 ).

Identification and Histological Observation of Endophytic Fungi Isolated from Medicinal Plant, Physalis angulata L.

“…Some endophytic fungi species isolated from P. angulata plant could be found in another plant. Colletotrichum alienum also found as one of the endophytic fungi species isolated from Hedycium acuminatum (Hastuti et al 2018), from Cananga odorata (Hastuti et al 2019a). Another species of Colletotrichum have been isolated from Piper nigrum, i.e: Colletotrichum gloeosporoides (Chitra et al 2014), in tomato fruit, i.e: Colletotrichum acutatum (Zivkovic et al 2010).…”

“…In the previous research it was found an endophytic fungi: Penicillium sp, isolated from Curcuma longa produced an antimicrobial compound (Singh et al 2014). Another research proved that some endophytic fungi, i.e: Fusarium oxysporum, Rhizoctonia sp., Fusarium solani, Colletotrichum alienum, Colletotrichum ti, Colletotrichum aotearoa, Aspergillus parasiticus, Hansfordia biophila, Fusarium semitectum, Colletotrichum coccodes, and Colletotrichum gloeosporoides isolated from Hedychium acuminatum could produced antibacterial compounds: alkaloid, flavonoid, terpenoid, and tannin in the secondary metabolites (Hastuti et al 2018). It is also proved that the secondary metabolites produced by those endophytic fungi species isolated from H. acuminantum have antimicrobial activity towards Staphylococcus aureus, and Bacillus subtilis (Hastuti et al 2019b).…”

“…It is also proved that the secondary metabolites produced by those endophytic fungi species isolated from H. acuminantum have antimicrobial activity towards Staphylococcus aureus, and Bacillus subtilis (Hastuti et al 2019b). The endophytic fungi live on the parenchyma cell wall and intercellular space of rhizome, epidermis, and parenchyma cell wall of leaf sheath, epidermis cell wall of H. acuminatum plant (Hastuti et al 2018). The histologic observation can be use to observe the endophytic fungi colonization in plant tissues.…”

The diversity of bacterial endophytes from Iris pseudacorus L. and their plant beneficial traits

“…It assumed that endophytes colonizing plant tissue play an important role in synthesizing of biologically active compounds and protecting plants from soil-borne disease ( Cho et al., 2015 ). The diversity of endophytic microbes associated with medicinal plant species such as Aloe vera, Mentha arvensis, Dracaena cochinchinensis, Hedycium acuminatum, Armoracia rusticana, Hypericum perforatum and others, and their biological activity were reported ( Salam et al., 2017 ; Hastuti et al., 2002 ; Egamberdieva et al., 2017 , 2020a ; Shurigin et al. 2020a ).…”

Diversity and biological activity of culturable endophytic bacteria associated with marigold (<i>Calendula officinalis</i> L.)

“…Endophytes use several mechanisms to support plant health, including the production of phytohormones [25] – [27] , 1-aminocyclopropane-1-carboxylate (ACC) deaminase [28] , hydrogen cyanide [29] , [30] and induced systemic resistance [31] – [33] . The diversity of endophytic microbes associated with medicinal plant species such as Aloe vera , Mentha arvensis , Dracaena cochinchinensis , Hedycium acuminatum, Armoracia rusticana , and others, were reported [34] – [36] . Although several studies reported on the phytochemical constituents and biological activity of marigold ( C. officinalis L.), to date, there have been no reports of endophytes associated with marigold and their biological properties.…”