Histological Alteration of Green Mussel Perna viridis Organs Exposed to Microplastics

Rahim, Nur Fadhilah; Yaqin, Khusnul

doi:10.15578/squalen.597

Cited by 7 publications

(2 citation statements)

References 72 publications

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“…This also takes place in wild samples (not undergone MPs‐experimental exposure) as demonstrated in green mussels by Vasanthi et al (2021). The results of other investigations show that MPs provoke the fusion of the two sides of the lamellae (Rahim & Yaqin, 2022). According to some authors, this could be due to the hyperplasia phenomenon (Carvalho et al, 2020).…”

Section: Discussionmentioning

confidence: 82%

The odd couple: Caffeine and microplastics. Morphological and physiological changes in Mytilus galloprovincialis

Impellitteri,

Briglia,

Porcino

et al. 2024

Microscopy Res & Technique

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In recent years, the presence of pharmaceuticals and microplastics (MPs) in aquatic ecosystems has raised concerns about their environmental impact. This study explores the combined effects of caffeine, a common pharmaceutical pollutant, and MPs on the marine mussel Mytilus galloprovincialis. Caffeine, at concentrations of 20.0 μg L−1, and MPs (1 mg L−1, 35–50 μm size range), was used to mimic real‐world exposure scenarios. Two hundred M. galloprovincialis specimens were divided into four groups: caffeine, MPs, Mix (caffeine + MPs), and Control. After a two‐week acclimation period, the mollusks were subjected to these pollutants in oxygen‐aerated aquariums under controlled conditions for 14 days. Histopathological assessments were performed to evaluate gill morphology. Cellular volume regulation and digestive gland cell viability were also analyzed. Exposure to caffeine and MPs induced significant morphological changes in M. galloprovincialis gills, including cilia loss, ciliary disk damage, and cellular alterations. The chitinous rod supporting filaments also suffered damage, potentially due to MP interactions, leading to hemocyte infiltration and filament integrity compromise. Hemocytic aggregation suggested an inflammatory response to caffeine. In addition, viability assessments of digestive gland cells revealed potential damage to cell membranes and function, with impaired cell volume regulation, particularly in the Mix group, raising concerns about nutrient metabolism disruption and organ function compromise. These findings underscore the vulnerability of M. galloprovincialis to environmental pollutants and emphasize the need for monitoring and mitigation efforts.Research Highlights The synergy of caffeine and microplastics (MPs) in aquatic ecosystems warrants investigation. MPs and caffeine could affect gill morphology of Mytilus galloprovincialis. Caffeine‐exposed cells had lower viability than the control group in the NR retention test. MPs and mix‐exposed cells struggled to recover their volume.

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