Histological analysis of the effects of a static magnetic field on bone healing process in rat femurs

Puricelli, Edela; Ulbrich, Lucienne Miranda; Ponzoni, Deise; Filho, João Julio da Cunha

doi:10.1186/1746-160x-2-43

Cited by 37 publications

(40 citation statements)

References 15 publications

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“…During vibration, the noise produced by the platform was minimal. The intensity of the static magnetic field generated by the platform was measured to be below 1 Gauss on the platform, which was far below the intensity reported to have osteogenic effects [30][31][32][33][34] or the safety level recommended by the Institute of Electrical and Electronics Engineers (IEEE). The thermal effect of the platform was not detected either on the platform surface or within the rat cage after 48 h of continuous running test.…”

Section: Methodsmentioning

confidence: 87%

Low‐magnitude high‐frequency vibration accelerates callus formation, mineralization, and fracture healing in rats

Leung

Shi

Cheung

et al. 2009

Journal Orthopaedic Research

104

130

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Fracture healing is a biological regenerative process that follows a well-orchestrated sequence. Most healing is uneventful and enhancement of normal fracture healing is not commonly done, although it is clinically important in the recovery and regain of functions after fracture. This study investigated the osteogenic effect of low-magnitude high-frequency vibration (LMHFV, 35 Hz, 0.3 g) on the enhancement of fracture healing in rats with closed femoral shaft fracture by comparing with sham-treated control. Assessments with plain radiography, micro-CT as well as histomorphometry showed that the amount of callus was significantly larger (p ¼ 0.001 for callus area, 2 weeks posttreatment); the remodeling of the callus into mature bone was significantly faster ( p ¼ 0.039, 4 weeks posttreatment) in the treatment group. The mechanical strength of the healed fracture in the treatment group at 4 weeks was significantly greater ( p < 0.001). The results showed the acceleration of callus formation, mineralization, and fracture healing in the treatment group. It is concluded that LMHFV enhances healing in the closed femoral shaft fracture in rats. The potential clinical advantages shall be confirmed in the subsequent clinical trials. ß

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Section: Methodsmentioning

confidence: 87%

Low‐magnitude high‐frequency vibration accelerates callus formation, mineralization, and fracture healing in rats

Leung

Shi

Cheung

et al. 2009

Journal Orthopaedic Research

104

130

View full text Add to dashboard Cite

show abstract

“…The present study followed the research line established by Puricelli [14]. As in many other experimental studies, the rat was used as a model, due to its advantages in terms of ease of acquisition, maintenance and surgical manipulation [16][17][18][19].…”

Section: Discussionmentioning

confidence: 99%

Histological evaluation of the influence of magnetic field application in autogenous bone grafts in rats

Puricelli

Dutra

Ponzoni

2009

International Journal of Oral and Maxillofacial Surgery

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“…SMF has been studied as a noninvasive physical treatment for promoting bone growth and bone healing, and preventing bone loss (Zhang et al, 2014a). Animal studies have revealed that certain SMFs have a synergistic role in such circumstances as bone surgical invasion (~50 mT) (Yan et al, 1998), ischemic bones (~50 mT) (Xu et al, 2001), ovariectomized rats (180 mT) (Xu et al, 2011), adjuvant arthritis rats (30-80 mT) (Taniguchi et al, 2004), bone fracture (4 mT) (Puricelli et al, 2006) and bone grafts (4 mT) (Puricelli et al, 2009). Most cellular studies indicate that the anabolic effects on bone homeostasis are due to the enhanced activities of osteoblast and promoted osteogenic differentiation in mesenchymal stem cells, although the underlying mechanisms of SMF effects on cell activities are still unclear (Colbert et al, 2009;Markov, 2007).…”

Section: Introductionmentioning

confidence: 99%

Regulation of osteoclast differentiation by static magnetic fields

Zhang

Meng

Ding

et al. 2016

Electromagnetic Biology and Medicine

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Static magnetic field (SMF) modulates bone metabolism, but little research is concerned with the effects of SMF on osteoclast. Our previous studies show that osteogenic differentiation is strongly correlated with magnetic strength from hypo (500 nT), weak (geomagnetic field, GMF), moderate (0.2 T) to high (16 T) SMFs. We speculated that the intensity that had positive (16 T) or negative (500 nT and 0.2 T) effects on osteoblast differentiation would inversely influence osteoclast differentiation. To answer this question, we examined the profound effects of SMFs on osteoclast differentiation from pre-osteoclast Raw264.7 cells. Here, we demonstrated that 500 nT and 0.2 T SMFs promoted osteoclast differentiation, formation and resorption, while 16 T had an inhibitory effect. Almost all the osteoclastogenic genes were highly expressed under 500 nT and 0.2 T, including RANK, matrix metalloproteinase 9 (MMP9), V-ATPase, carbonic anhydrase II (Car2) and cathepsin K (CTSK), whereas they were decreased under 16 T. In addition, 16 T disrupted actin formation with remarkably decreased integrin β3 expression. Collectively, these results indicate that osteoclast differentiation could be regulated by altering the intensity of SMF, which is just contrary to that on osteoblast differentiation. Therefore, studies of SMF effects could reveal some parameters that could be used as a physical therapy for various bone disorders.

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Histological analysis of the effects of a static magnetic field on bone healing process in rat femurs

Abstract: Background: The aim of this study was to investigate, in vivo, the quality of bone healing under the effect of a static magnetic field, arranged inside the body.

Cited by 37 publications

References 15 publications

Low‐magnitude high‐frequency vibration accelerates callus formation, mineralization, and fracture healing in rats

Low‐magnitude high‐frequency vibration accelerates callus formation, mineralization, and fracture healing in rats

Histological evaluation of the influence of magnetic field application in autogenous bone grafts in rats

Regulation of osteoclast differentiation by static magnetic fields

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