Regulation of osteoclast differentiation by static magnetic fields

Abstract

Static magnetic field (SMF) modulates bone metabolism, but little research is concerned with the effects of SMF on osteoclast. Our previous studies show that osteogenic differentiation is strongly correlated with magnetic strength from hypo (500 nT), weak (geomagnetic field, GMF), moderate (0.2 T) to high (16 T) SMFs. We speculated that the intensity that had positive (16 T) or negative (500 nT and 0.2 T) effects on osteoblast differentiation would inversely influence osteoclast differentiation. To answer this question, we examined the profound effects of SMFs on osteoclast differentiation from pre-osteoclast Raw264.7 cells. Here, we demonstrated that 500 nT and 0.2 T SMFs promoted osteoclast differentiation, formation and resorption, while 16 T had an inhibitory effect. Almost all the osteoclastogenic genes were highly expressed under 500 nT and 0.2 T, including RANK, matrix metalloproteinase 9 (MMP9), V-ATPase, carbonic anhydrase II (Car2) and cathepsin K (CTSK), whereas they were decreased under 16 T. In addition, 16 T disrupted actin formation with remarkably decreased integrin β3 expression. Collectively, these results indicate that osteoclast differentiation could be regulated by altering the intensity of SMF, which is just contrary to that on osteoblast differentiation. Therefore, studies of SMF effects could reveal some parameters that could be used as a physical therapy for various bone disorders.

Disorder of Iron Metabolism Inhibits the Recovery of Unloading-Induced Bone Loss in Hypomagnetic Field

“…However, our recent research indicated that the effects of HyMF on bone cannot be overlooked. In vitro, we found that HyMF can inhibit osteoblast differentiation and mineralization, and promote osteoclast formation and bone resorption . In vivo, HyMF aggravated bone loss induced by hindlimb unloading (HLU) in rats and mice .…”

Effects of static magnetic fields on eukaryotic cytoskeleton

“…(a) 9 T static magnetic field makes the Paramecium caudatum move along the direction of magnetic field; (b) the trajectory of Paramecium caudatum in a series of static magnetic fields (i), and the anisotropy of diamagnetic susceptibility were calculated from the relationship between magnetic field and trajectory (ii) [50] ; (c) 8 T static magnetic field causes the Paramecium cilia parallel to the magnetic field, and Ctr is control group [44] 响其与微丝的相互作用. 2017年, Zhang等人 [59] [54] Figure 6 Static magnetic fields affect the self-assembly process of G-actin protein in vitro. (a) Optical micrograph of the G-actin paracrystals in 10 T static magnetic field.…”

Transcriptome Analysis Reveals the Negative Effect of 16 T High Static Magnetic Field on Osteoclastogenesis of RAW264.7 Cells

“…In contrast, HiSMF (16 T) had a negative effect, which is related with the downregulated expression of osteoclastogenic genes such as matrix metalloproteinase 9 (MMP9), V-ATPase, carbonic anhydrase II (Car2), and RANK. Furthermore, HiSMF altered osteoclast cytoskeleton organization, which destructed the formation of filamentous actin ring and downregulated the expression of integrin β3 [18].…”