Histological and autoradiographic findings in cryonecrosis of the liver and kidney

Breining, H; Helpap, B.; Minderjahn, A; Lymberopoulos, S

doi:10.1016/0011-2240(74)90121-7

Cited by 21 publications

(5 citation statements)

References 16 publications

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“…Early experimental work by Breining and colleagues and Sindelar and associates helped shed light on the histologic and ultrastructural changes after renal cryoablation, respectively. 9,24 In the swine model, Chosy and coworkers 7 demonstrated that complete renal-tissue ablation occurs when the target temperature is Յ Ϫ19.4°C. However, a clear understanding of the experimental data is necessary prior to clinical application.…”

Section: Discussionmentioning

confidence: 99%

Cryotherapy of Renal Masses: Intermediate-Term Follow-Up

Moinzadeh

Spaliviero²,

Gill³

2005

Journal of Endourology

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Minimally invasive procedures for the management of small renal masses are increasingly being performed. Of the probe ablative modalities, renal cryotherapy is the most studied in the laboratory and the clinical setting. In this paper, we briefly review the principles of cryotherapy for the treatment of renal tumors. Current published series are outlined, with emphasis on intermediate oncologic follow-up.

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Section: Discussionmentioning

confidence: 99%

Cryotherapy of Renal Masses: Intermediate-Term Follow-Up

Moinzadeh

Spaliviero²,

Gill³

2005

Journal of Endourology

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“…Ten years later the histological and autoradiographic effects of direct cryonecrosis of the kidney were described by Breining et al . [22] The feasibility of renal cryoablation using a needle‐delivery system of pressurized liquid nitrogen as well as a detailed description of the microscopic and ultrastructural changes in the rat kidney were later reported by Sindelar et al . [13] Additional data, including the temperature characteristics of the iceball and the gross and histological effects of renal cryoablation over time, were described in porcine models [14,23].…”

Section: Discussionmentioning

confidence: 99%

A novel murine model for the study of human renal cryoablation

et al. 2007

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OBJECTIVE To develop a reproducible, non‐debilitating in vivo murine model of human renal cryoablation using a standard closed argon‐delivery system. MATERIALS AND METHODS Custom engineered 2‐mm conical tip cryoprobes for use on the standard argon‐based cryoablation unit (Endocare, Inc. Irvine, CA, USA) were used to create small controllable iceballs (−160 °C) in the mouse kidney. The time to create a 4‐mm cryolesion was compared using a contact vs puncture technique in 10 mice. To show consistency of the induced‐freeze injury, a 4‐mm iceball was created in 20 murine renal units and the time to creation and the size of the resultant cryolesion measured. To investigate lesion regression and histological changes, we created a 4‐mm renal cryolesion in 28 mice and killed four each at 1, 3, 7, 14, 21, 28, and 35 days. The measured coronal cross‐sectional area of the cryoablation site at necroscopy was compared to the initial calculated area as a percentage. To assess renal preservation, blood urea nitrogen (BUN) and creatinine levels at 1 week after cryoablation or sham ablation was compared (10 mononephric mice in each group). RESULTS The time to create the desired iceball was 1.9 times quicker using the puncture vs the contact technique. The mean (sd) time to forming a 4‐mm iceball was 35.3 (4.8) s with a mean maximum length of the resultant post‐thaw injury of 5.7 (0.5) mm and a 9% coefficient of variance. Regression analysis of the two‐dimensional cross‐sectional coronal area of the cryolesion showed a statistically significant linear pattern of regression over time (P = 0.037) and classic histological findings. There was no significant difference in the BUN or creatinine levels in mononephric mice 1 week after cryotherapy compared with the sham‐ablated controls. CONCLUSIONS We describe a reproducible, non‐debilitating, easily manipulated murine model for the study of human renal cryoablation.

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“…By 2 weeks after freezing, ultrastructural ex-Cryosurgery has come increasingly under evaluation as a modality for the treatment of certain benign and malignant tumors [ 5 ] , and selective freezing of lesions in the genitourinary tract has begun to be examined [2,3,6]. Cryosurgery may be considered as an approach to the treatment of certain lesions of the kidney because of the ability of cryosurgery to produce sharply demarcated necrosis in neoplastic areas without affecting adjacent normal tissue and invoking only a mild inflammatory response [4,9]. Relatively little work has been done, however, in histologically documenting changes following cryoinjury to normal tissues.…”

Section: Discussionmentioning

confidence: 99%

“…Certain characteristics of cryosurgery may be advantageous in tumor treatment. The cryolesion characteristically has sharp lines of demarcation of cellular damage associated with a relatively mild inflammatory response [3,4,9]. The possibility of enhanced host immunological response following freezing has been proposed in certain animal model tumor systems [1,7].…”

Section: Introductionmentioning

confidence: 99%

Histological and ultrastructural changes in rat kidney after cryosurgery

Sindelar

Javadpour

Bagley

1981

Journal of Surgical Oncology

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Effect of cryoinjury to normal rat kidney was studied at the light and electron microscope level, and characterization of the time course of pathologic changes in kidney tissue after cryosurgery was described from 1 hr up to 14 days after production of a standard cryolesion. Cryoinjury to the kidney resulted in sharply demarcated lesions with minimal inflammatory response during the period of cell destruction and healing by fibrosis. Cellular protein was completely coagulated within 1 hr after cryoinjury, with crystallization of protein and disruption of membranes. Cellular disruption was rapid, and the clearance of cellular debris from the area of injury by macrophage infiltration was accomplished through a quite mild inflammatory reaction. Supporting stromal elements of renal parenchyma remained intact, including collagen tubular framework and basement membranes, and formed the lattice over which fibrosis developed.

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Histological and autoradiographic findings in cryonecrosis of the liver and kidney

Cited by 21 publications

References 16 publications

Cryotherapy of Renal Masses: Intermediate-Term Follow-Up

Cryotherapy of Renal Masses: Intermediate-Term Follow-Up

A novel murine model for the study of human renal cryoablation

Histological and ultrastructural changes in rat kidney after cryosurgery

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