Histological and Histometric Evaluation of the Liver in Astyanax Bimaculatus (Teleostei: Characidae), Exposed to Different Concentrations of an Organochlorine Insecticide

Abstract

To investigate possible morphological changes to the liver tissue of lambaris, Astyanax bimaculatus (Linnaeus, 1758), females were exposed to treatments of sublethal concentrations of the insecticide Thiodan V R for 96 hr. Treatments included three sublethal concentrations of 1.15, 2.3, and 5.6 lg L 21 of Thiodan V R and a control group without insecticide. The action of Thiodan V R at sublethal concentrations did not affect the morphological structure of the liver as a whole, but changes in isolated locations of the hepatic parenchyma were observed. Glycogen depletion, nuclear and cytoplasmic deformation, nuclear and cytoplasmic hypertrophy, hyperemia, and cellular degeneration in liver cells at the different concentrations studied were recorded. These observed changes in the livers were greater in groups exposed to Thiodan V R in comparison to the control group. Furthermore, there was a change in the diameter of the nuclei and cytoplasm of hepatocytes in the different treatments. The groups exposed to Thiodan V R also exhibited a larger number of hepatocyte nuclei and a reduction in the amount of cytoplasm. We conclude that for the exposure period and concentrations of Thiodan V R analyzed, the morphology of hepatic tissue had a cellular adaptive response.

Growth, Chemical Composition, Histology and Antioxidant Genes of Atlantic Salmon (Salmo salar) Fed Whole or Pre-Processed Nannochloropsis oceanica and Tetraselmis sp.

“…Vacuoles were visible in the hepatocytes of the pre-extruded groups (NE, TE). These vacuoles may indicate accumulation of glycogen or fat in the hepatocytes prompted by the algae incorporation in the feeds, as noted in previous studies [74,75]. A study revealed that the vacuoles in the liver of juvenile Senegalese sole (Solea senegalensis) disappeared after 90 days of feeding of the macroalga, Ulva ohnoi [76].…”

Histopathological analysis of liver of the catfish <i>Pimelodus maculatus</i> in a tropical eutrophic reservoir from Southeastern Brazil

“…Among the species that have been studied in this subject, we can highlight the histology of the liver of the channel catfish Ictalurus punctatus (Rafinesque, 1818), the tigerfish Hydrocynus forskahlii (Cuvier, 1819), the trout Oncorhynchus mykiss (Walbaum, 1792), the goldfish Carassius auratus (Linnaeus 1758) and the Siberian sturgeon Acipenser baerii (Brandt, 1869) (Ostaszewska et al, 2016). However, information on liver tissues in tropical freshwater systems are scarce (e.g., Bombonato, Rochel, Vicentini, & Vicentini, 2007;Faccioli, Chedid, Bombonato, Vicentini, & Vicentini, 2014;Marcon, Bazzoli, Mounteer, & Benjamin, 2015). As most of Brazilian freshwater systems are undergoing increasing pollution, especially eutrophication, there is a need for studies on this subject on eutrophic systems.…”

Growth hormone on ovarian morphology of lambaris (Astyanax bimaculatus) after induced spawning

“…Revisions have considered many of the 88 genera (620 species) of Characidae as "Incertaesedis", with 86 species belonging to the genus Astyanax (LIMA et al, 2003). Several papers have reported the importance of lambari to the trophic structure of Brazilian ecosystems and their reproductive behavior in the face of environmental change (PRADO et al, 2011;MARCON et al, 2015a). Lambari is of great importance to the equilibrium of the ecosystems where they occur, namely rivers of South and Central America, and they are well represented in such ecosystems in Brazilian (CARVALHO et al, 2009;WEBER et al, 2013).…”

“…Counts for each section were done in 10 randomly selected fields for each animal in both groups using a microscope equipped with a micrometer reticle (1 mm) in a 10X objective. Granulocytes associated with atretic follicles were also counted in 10 randomly selected fields using a microscope with a 40X objective (MARCON et al, 2015a).…”