Histological and sex steroid hormone receptor changes in testes of immature, mature, and aged chickens

González-Morán, María Genoveva; Guerra‐Araiza, Christian; Campos, Marı́a G.; Camacho‐Arroyo, Ignacio

doi:10.1016/j.domaniend.2008.08.001

Cited by 52 publications

(27 citation statements)

References 45 publications

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“…2). These results are in agreement with previous published data for these receptors in birds (Gonzalez-Moran et al 2008. A significant increase (26%) in ESR2 expression was observed in the epididymal region of roosters affected by epididymal lithiasis compared with non-affected animals (Fig.…”

Section: Esr1 and Esr2 Detectionsupporting

confidence: 83%

Roosters affected by epididymal lithiasis present local alteration in vitamin D3, testosterone and estradiol levels as well as estrogen receptor 2 (β) expression

Oliveira

Dornas²,

Praes³

et al. 2011

Reproduction

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Epididymal lithiasis is a reproductive dysfunction of roosters that is associated with loss of fertility and is characterized by the formation of calcium stones in the lumen of the efferent ductules of the epididymal region. The efferent ductules of birds are responsible for the reabsorption of the fluid coming from the testis as well as luminal calcium. It has been hypothesized that the epididymal stone formation may be related to the impairment of local fluid or calcium homeostasis, which depends on hormones such as estradiol (E 2 ). Therefore, this study aimed to investigate possible alterations in the expression of ERa (ESR1) and ERb (ESR2) in the epididymal region of roosters affected by epididymal lithiasis. The study was performed by immunohistochemistry and western blotting assays. In addition, the concentrations of E 2 , vitamin D3, and testosterone, which are also key hormones in maintenance of calcium homeostasis, were determined in the plasma and epididymal region, by ELISA. It was observed that ESR2 expression is increased in all segments of the epididymal region of affected roosters, whereas ESR1 levels are not altered. Moreover, the hormone concentration profiles were changed, as in the epididymal region of roosters with lithiasis the E 2 levels were increased and vitamin D3 as well as testosterone concentrations were significantly decreased. These results suggest that a hormonal imbalance may be involved with the origin and progression of the epididymal lithiasis, possibly by affecting the local fluid or calcium homeostasis.

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Section: Esr1 and Esr2 Detectionsupporting

confidence: 83%

Roosters affected by epididymal lithiasis present local alteration in vitamin D3, testosterone and estradiol levels as well as estrogen receptor 2 (β) expression

Oliveira

Dornas²,

Praes³

et al. 2011

Reproduction

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show abstract

“…However, in all vertebrates, PGRs are expressed predominantly in the reproductive organ (Graham & Clarke 1997, Wang et al 2004, González-Morán et al 2008. Interestingly, pgr mRNA has a less broad expression pattern than the androgen receptor in male teleosts (de Waal et al 2008), although both receptor types were created from a 3-ketogonadal steroid receptor by the third genome duplication during vertebrate evolution (Thornton 2001).…”

Section: Discussionmentioning

confidence: 99%

Cloning, pharmacological characterization, and expression analysis of Atlantic salmon (Salmo salar L.) nuclear progesterone receptor

Chen¹,

Bogerd²,

Andersson³

et al. 2011

Reproduction

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To better understand the role(s) of progestogens during early stages of spermatogenesis, we carried out studies on the nuclear progesterone receptor (Pgr) of the Atlantic salmon. Its open-reading frame shows the highest similarity with other piscine Pgr proteins. When expressed in mammalian cells, salmon Pgr exhibited progestogen-specific, dose-dependent induction of reporter gene expression, with 17a,20b-dihydroxy-4-pregnen-3-one (DHP) showing the highest potency. We then analyzed testicular pgr mRNA and DHP plasma levels in animals during the onset of spermatogenesis, which were exposed to natural light or to constant light, to induce significant differences in testis growth. Grouping of the animals according to their progress through spermatogenesis showed that testicular pgr mRNA levels as well as DHP plasma levels first increased when germ cells had reached the stage of late type B spermatogonia and further increased when entered meiosis, i.e. when spermatocytes were present. However, in situ hybridization studies revealed that pgr mRNA expression was restricted to Sertoli cells, with a strong signal in Sertoli cells contacting type A/early type B spermatogonia, while Sertoli cells contacting larger germ cell clones with further differentiated stages (e.g. late type B spermatogonia) were less intensely/not stained. We conclude that the increase in pgr mRNA levels per pair of testis reflects, at least in part, the increased number of Sertoli cells enveloping type A and early type B spermatogonia. We propose that Sertoli cell-expressed Pgr may mediate DHP-stimulated early steps in spermatogenesis in Atlantic salmon, such as an increase in the number of new spermatogonial cysts.

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“…Studies on sex hormone receptors have mainly focused on vertebrates (Fannon et al, 2001;González-Morán et al, 2008;Cleve et al, 2012;Wang et al, 2014;Fu et al, 2016); however, the number of studies that have investigated sex hormone receptors in invertebrates has increased in the last 20 years, before which it was believed that these receptors originated from deuterostome (Laudet et al, 1992). These receptor families are more ancient than previously thought (Thornton et al, 2003).…”

Section: Introductionmentioning

confidence: 96%

Immunohistochemical localization of sex hormone receptors in two Raillietina tapeworms

L¹,

Sun²,

Mu³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Sex hormone receptors play critical roles in development and reproduction. However, it is not known whether they exist in Raillietina tapeworms, and if they do, whether they have a similar function to that in vertebrates. We examined the immunohistochemical distributions of androgen receptors (ARs), estrogen receptors (ERs), and progesterone receptors (PRs) in the tissues of two tapeworm species: Raillietina echinobothrida and Raillietina tetragona. Immunopositive ARs were found in the entire reproductive system of R. echinobothrida, including the testes, ovaries, and oocysts, and weakly immunopositive ERs and PRs were found in the testes, ovaries, and oocysts. Immunopositive ARs were also found throughout the entire reproductive system of R. tetragona, including the testes, ovaries, and oocysts, and weakly immunopositive ERs were in the testes and oocysts; the PRs were distributed in an immunonegative manner. The results show that androgens and their receptors play critical roles in reproductive system development in the two tapeworms. The immunoreactivity and tissue localizations of the sex hormone receptors suggest that, in both species, they have similar functions as in vertebrates, and modulate reproduction.

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Histological and sex steroid hormone receptor changes in testes of immature, mature, and aged chickens

Cited by 52 publications

References 45 publications

Roosters affected by epididymal lithiasis present local alteration in vitamin D3, testosterone and estradiol levels as well as estrogen receptor 2 (β) expression

Roosters affected by epididymal lithiasis present local alteration in vitamin D3, testosterone and estradiol levels as well as estrogen receptor 2 (β) expression

Cloning, pharmacological characterization, and expression analysis of Atlantic salmon (Salmo salar L.) nuclear progesterone receptor

Immunohistochemical localization of sex hormone receptors in two Raillietina tapeworms

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