Histological and three-dimensional evaluation of osseointegration to nanostructured calcium phosphate-coated implants

Jimbo, Ryo; Coelho, Paulo G.; Vandeweghe, Stefan; Schwartz-Filho, Humberto Osvaldo; Hayashi, Mariko; Ono, Daisuke; Andersson, Martin; Wennerberg, Ann

doi:10.1016/j.actbio.2011.07.017

Cited by 76 publications

(62 citation statements)

References 32 publications

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“…Especially when evaluating the effect of nanometer structures, detailed evaluation approaches are essential to clarify their roles during osteogenesis (Jimbo et al, 2011a;Jimbo et al, 2011b). In our previous study, we reported that the presence of HA nanotopography on implant surfaces enhanced osteogenic markers such as alkaline phosphatase and osteocalcin and at the same time suppresses inflammation.…”

Section: Discussionmentioning

confidence: 99%

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Nano Hydroxyapatite-coated Implants Improve Bone Nanomechanical Properties

et al. 2012

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Abstract:Nanostructure modification of dental implants has long been sought as a mean to improve osseointegration through enhanced biomimicry of host structures. Several methods have been proposed and demonstrated for creating nanotopographic features; here we described a nanoscale hydroxyapatite (HA) coated implant surface and hypothesized that it will hasten osseointegration and improve its quality relative to non-coated implants. Twenty threaded titanium alloy implants, half prepared with stable HA nanoparticle surface and half grit-blasted, acid-etched, and heat treated (HT), were inserted into rabbit femurs. Preoperatively, the implants were morphologically and topographically characterized. After 3 weeks of healing, the samples were retrieved for histomorphometry. Moreover, the nanomechanical properties of the surrounding bone were evaluated using nanoindentation. While both implants revealed similar bone-to-implant contact, the nanoindentation demonstrated that the tissue quality was significantly enhanced around the HA-coated implants, validating the postulated hypothesis.

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Section: Discussionmentioning

confidence: 99%

“…Therefore, three-dimensional evaluation using the micro computed tomography has been implemented in order to compensate and to obtain further detailed information (Jimbo et al, 2011a). In this study, the effect of nanostructured HA-coating was evaluated histologically.…”

Section: Introductionmentioning

confidence: 99%

Nano Hydroxyapatite-coated Implants Improve Bone Nanomechanical Properties

et al. 2012

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“…Indeed, an important aim of the present study was to reveal molecular processes that underlie the observed biomechanics, by combining information obtained from biomechanical testing with that obtained from gene expression analysis. Based on the previously reported histological and biomechanical studies (Bjursten et al, 2010;Jimbo et al, 2011a), it is suggested that the effect of nanotopography in terms of bone acceleration occurs early. Therefore, in this study, we focused on the genetic responses at 2 and 4 wks, which is a commonly used time frame to evaluate initial osseointegration.…”

Section: Discussionmentioning

confidence: 99%

Genetic Responses to Nanostructured Calcium-phosphate-coated Implants

et al. 2011

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Nanostructured calcium phosphate (CaP) has been histologically and biomechanically proven to enhance osseointegration of implants; however, conventional techniques were not sufficiently sensitive to capture its biological effects fully. Here, we compared the conventional removal torque (RTQ) evaluation and gene expression in tissues around nanostructured CaP-coated implants, using real-time RT-PCR, with those of uncoated implants, in a rabbit model. At 2 wks, RTQ values were significantly higher, alkaline phosphatase (ALP) expression was significantly higher, and runt-related transcription factor 2 and tumor necrosis factor-α expressions were significantly lower in the coated than in the uncoated implants. This indicates that inflammatory responses were suppressed and osteoprogenitor activity increased around the CaP-coated surface. At 4 wks, although RTQ values did not significantly differ between the 2 groups, ALP and osteocalcin (OCN) were significantly up-regulated in the coated group, indicating progressive mineralization of the bone around the implant. Moreover, an osteoclast marker, adenosine triphosphatase, which indicates acidification of the resorption lacunae, was significantly higher for the coated implants, suggesting gradual resorption of the CaP coating. This study reveals detailed genetic responses to nanostructured CaP-coated implants and provides evidence that the effect of nanotopography is significant during the osseointegration cascade.

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“…In vivo studies also indicated that CaP coating on implant surface can enhance osseointegration and fixation. 50,51 Thus, the CaP coating may work synergistically with the loaded alendronate in enhancing osteoblast functions while not affecting the alendronate's inhibiting effect on fibroblast proliferation.…”

Section: Osteoblast Attachment Proliferation Differentiation and Amentioning

confidence: 99%

AnIn VitroAssessment of Fibroblast and Osteoblast Response to Alendronate-Modified Titanium and the Potential for Decreasing Fibrous Encapsulation

Neoh

Shi

et al. 2013

Tissue Engineering Part A

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Fibrous encapsulation can impair implant osseointegration and cause implant failure but currently there are limited strategies to address this problem. Since bisphosphonates (BPs), a class of drugs widely used to treat bone diseases, was recently found to induce fibroblast apoptosis, we hypothesize that by loading BPs on titanium (Ti) implant surface, fibrous encapsulation may be inhibited with simultaneous enhancement of implant osseointegration. This strategy of local administration can also be expected to minimize the adverse side effects of BPs, which are associated with intravenous injections. To verify this hypothesis, alendronate was loaded on Ti surface via a hydroxyapatite (CaP) coating, and the effects of the loaded alendronate on fibroblast proliferation and apoptosis, and osteoblast proliferation, alkaline phosphatase (ALP) activity, and apoptosis were investigated in vitro. With a surface density of loaded alendronate 0.046 mg/cm 2 or higher, fibroblast proliferation was suppressed due to increased apoptosis, while osteoblast proliferation and ALP activity increased with minimal apoptosis. In a coculture of fibroblasts and osteoblasts in a 1:1 ratio, *60% of the cells on these alendronate-loaded substrates were osteoblasts 1 day after cell seeding. The percentage of osteoblasts increased to about 75% 4 days after cell seeding. These results suggest that fibroblasts and osteoblasts respond differently toward the alendronate-modified substrates, and this phenomenon can potentially be capitalized to reduce fibrous encapsulation.

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Histological and three-dimensional evaluation of osseointegration to nanostructured calcium phosphate-coated implants

Cited by 76 publications

References 32 publications

Nano Hydroxyapatite-coated Implants Improve Bone Nanomechanical Properties

Nano Hydroxyapatite-coated Implants Improve Bone Nanomechanical Properties

Genetic Responses to Nanostructured Calcium-phosphate-coated Implants

AnIn VitroAssessment of Fibroblast and Osteoblast Response to Alendronate-Modified Titanium and the Potential for Decreasing Fibrous Encapsulation

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