Histological and transcriptional effects of androstenedione in adult zebrafish (<scp><i>Danio rerio</i></scp>)

Liang, Yan‐Qiu; Pan, Chang-Gui; Xie, Linchun; Huo, Jialiang; Yang, Jingquan; Lan, Tianhong; Dong, Zhongdian; Duan, Meina

doi:10.1002/tox.23064

Cited by 4 publications

(3 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 65 For the testis, our primary focus was on different developmental stages of the spermatogenic cycle, including spermatogonia, spermatocytes, spermatids, and spermatozoa. 66 …”

Section: Methodsmentioning

confidence: 99%

Combined Effects of Polystyrene Nanosphere and Homosolate Exposures on Estrogenic End Points in MCF-7 Cells and Zebrafish

Ye,

Li,

Xian

et al. 2024

Environ Health Perspect

View full text Add to dashboard Cite

Background: Micro- and nanoplastics (MNPs) and homosalate (HMS) are ubiquitous emerging environmental contaminants detected in human samples. Despite the well-established endocrine-disrupting effects (EDEs) of HMS, the interaction between MNPs and HMS and its impact on HMS-induced EDEs remain unclear. Objectives: This study aimed to investigate the influence of MNPs on HMS-induced estrogenic effects and elucidate the underlying mechanisms in vitro and in vivo . Methods: We assessed the impact of polystyrene nanospheres (PNSs; , ) on HMS-induced MCF-7 cell proliferation (HMS: , equivalent to ) using the E-SCREEN assay and explored potential mechanisms through transcriptomics. Adult zebrafish were exposed to HMS ( ) with or without PNSs ( , ) for 21 d. EDEs were evaluated through gonadal histopathology, fertility tests, steroid hormone synthesis, and gene expression changes in the hypothalamus–pituitary–gonad–liver (HPGL) axis. Results: Coexposure of HMS and PNSs resulted in higher expression of estrogen receptor ( ESR1 ) and the mRNAs of target genes ( pS2 , AREG , and PGR ), a greater estrogen-responsive element transactivation activity, and synergistic stimulation on MCF-7 cell proliferation. Knockdown of serum and glucocorticoid-regulated kinase 1 (SGK1) rescued the MCF-7 cell proliferation induced by PNSs alone or in combination with HMS. In zebrafish, coexposure showed higher expression of SGK1 and promoted ovary development but inhibited spermatogenesis. In addition, coexposure led to lower egg hatchability, higher embryonic mortality, and greater larval malformation. Coexposure also modulated steroid hormone synthesis genes ( cyp17a2 , hsd17 1 , esr2b , vtg1 , and vtg2 ), and resulted in higher ( ) release in females. Conversely, males showed lower testosterone, , and gene expressions of cyp11a1 , cyp11a2 , cyp17a1 , cyp17a2 , and hsd17 1 . Discussion: PNS exposure exacerbated HMS-induced estrogenic effects via SGK1 up-regulation in MCF-7 cells and disrupting the HPGL axis in zebrafish, with gender-specific patterns. This offers new mechanistic insights and health implications of MNP and contaminant coexposure. https://doi.org/10.1289/EHP13696

show abstract

“… 65 For the testis, our primary focus was on different developmental stages of the spermatogenic cycle, including spermatogonia, spermatocytes, spermatids, and spermatozoa. 66 …”

Section: Methodsmentioning

confidence: 99%

Combined Effects of Polystyrene Nanosphere and Homosolate Exposures on Estrogenic End Points in MCF-7 Cells and Zebrafish

Ye,

Li,

Xian

et al. 2024

Environ Health Perspect

View full text Add to dashboard Cite

show abstract

“…All experiments were performed in at least triplicate with three biological repetitions. Differences were calculated according to the 2 −△△Ct comparative quantization method using 18S as an internal control 36 …”

Section: Methodsmentioning

confidence: 99%

“…Differences were calculated according to the 2 À44Ct comparative quantization method using 18S as an internal control. 36…”

Section: Quantitative Rt-qpcr Analysismentioning

confidence: 99%

Intergenerational toxic effects of parental exposure to [C_nmim]NO₃ (n = 2,4,6) on nervous and skeletal development in zebrafish offspring

Liu

et al. 2023

Environmental Toxicology

View full text Add to dashboard Cite

Ionic liquids (ILs) are thought to have negative effects on human health. Researchers have explored the effects of ILs on zebrafish development during the early stages, but the intergenerational toxicity of ILs on zebrafish development has rarely been reported. Herein, parental zebrafish were exposed to different concentrations (0, 12.5, 25, and 50 mg/L) of [Cnmim]NO3 (n = 2, 4, 6) for 1 week. Subsequently, the F1 offspring were cultured in clean water for 96 h. [Cnmim]NO3 (n = 2, 4, 6) exposure inhibited spermatogenesis and oogenesis in F0 adults, even causing obvious lacunae in the testis and atretic follicle oocytes in ovary. After parental exposure to [Cnmim]NO3 (n = 2, 4, 6), the body length and locomotor behavior were measured in F1 larvae at 96 hours post‐fertilization (hpf). The results showed that the higher the concentration of [Cnmim]NO3 (n = 2, 4, 6), the shorter the body length and swimming distance, and the longer the immobility time. Besides, a longer alkyl chain length of [Cnmim]NO3 had a more negative effect on body length and locomotor behavior. RNA‐seq analysis revealed several downregulated differentially expressed genes (DEGs)—grin1b, prss1, gria3a, and gria4a—enriched in neurodevelopment‐related pathways, particularly the pathway for neuroactive ligand–receptor interaction. Moreover, several upregulated DEGs, namely col1a1a, col1a1b, and acta2, were mainly associated with skeletal development. Expression of DEGs was tested by RT‐qPCR, and the outcomes were consistent with those obtained from RNA‐Seq. We provide evidence showing the effects of parental exposure to ILs on the regulation of nervous and skeletal development in F1 offspring, demonstrating intergenerational effects.

show abstract

Exposure of adult zebrafish to androstenedione alters thyroid hormone levels and the transcriptional expression of genes related to the hypothalamus-pituitary-thyroid axis

Liang

Situ

Xie

et al. 2021

Aquaculture Reports

View full text Add to dashboard Cite

Histological and transcriptional effects of androstenedione in adult zebrafish (Danio rerio)

Cited by 4 publications

References 35 publications

Combined Effects of Polystyrene Nanosphere and Homosolate Exposures on Estrogenic End Points in MCF-7 Cells and Zebrafish

Combined Effects of Polystyrene Nanosphere and Homosolate Exposures on Estrogenic End Points in MCF-7 Cells and Zebrafish

Intergenerational toxic effects of parental exposure to [C_nmim]NO₃ (n = 2,4,6) on nervous and skeletal development in zebrafish offspring

Exposure of adult zebrafish to androstenedione alters thyroid hormone levels and the transcriptional expression of genes related to the hypothalamus-pituitary-thyroid axis

Contact Info

Product

Resources

About

Histological and transcriptional effects of androstenedione in adult zebrafish (Danio rerio)

Cited by 4 publications

References 35 publications

Combined Effects of Polystyrene Nanosphere and Homosolate Exposures on Estrogenic End Points in MCF-7 Cells and Zebrafish

Combined Effects of Polystyrene Nanosphere and Homosolate Exposures on Estrogenic End Points in MCF-7 Cells and Zebrafish

Intergenerational toxic effects of parental exposure to [Cnmim]NO3 (n = 2,4,6) on nervous and skeletal development in zebrafish offspring

Exposure of adult zebrafish to androstenedione alters thyroid hormone levels and the transcriptional expression of genes related to the hypothalamus-pituitary-thyroid axis

Contact Info

Product

Resources

About

Intergenerational toxic effects of parental exposure to [C_nmim]NO₃ (n = 2,4,6) on nervous and skeletal development in zebrafish offspring