Histological and Ultrastructural Study Of Silver Nanoparticles Toxicity and The Possible Protective Effect Of Vitamin C On Submandibular Salivary Glands Of Albino Rats

Shamel, Mohamed; Ankily, Mahmoud Al

doi:10.19070/2377-8075-21000428

Cited by 3 publications

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“…In the current study, it was observed that the excretory ducts showed a loss of pseudo stratification. These results are similar to those obtained by Shamel et al (2021) [ 29 ], where they found that the excretory ducts of the submandibular salivary glands of albino rats appeared vacuolated and degenerated and lost their pseudo stratification after administration of AgNPs. The ultrastructural results of the AgNPs group support the previously mentioned histological results, which showed severe ultra-structural changes of the secretory portions and ducts as well as the CT stroma.…”

Section: Discussionsupporting

confidence: 92%

“…The most noticeable observation was the frequent formation of cytoplasmic vacuolization in the serous acini as well as in the intraglandular ducts. These histological changes were also observed by several studies where rats were intoxicated with AgNPs [ 29 , 50 ]. These findings were explained by Asharni et al (2009) [ 51 ], who found that AgNPs can cause DNA and chromosomal abnormalities.…”

Section: Discussionsupporting

confidence: 68%

“…In fact, AgNPs toxicity has been reported in many tissues, such as liver, kidneys, intestine, and reproductive systems [ 45 , 46 , 47 , 48 ]. Oral tissue toxicity following AgNPs exposure has been previously reported in salivary glands and lingual mucosa [ 29 , 49 ]. In the current study, evidence of AgNPs toxicity in the submandibular salivary glands was evident through histological, immunohistochemical, and ultrastructural results.…”

Section: Discussionmentioning

confidence: 99%

“…However, many studies have reported potential toxicological effects of AgNPs. Those studies have proposed that AgNPs possess a possible cytotoxic effect through the generation of oxidative stress induced by the overproduction of reactive oxygen species (ROS) in various tissues and pathological conditions including cancer [ 25 , 26 , 27 , 28 , 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

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Attenuating Effect of Vitamin E against Silver Nano Particles Toxicity in Submandibular Salivary Glands

et al. 2021

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Silver nanoparticles (AgNPs) are extensively used in many industries due to their superior antimicrobial properties. However, it is evident from many studies that AgNPs has cytotoxic potential through its effect on excessive formation of reactive oxygen species (ROS). The aim of this study was to examine the toxic effect of AgNPs on the submandibular salivary glands and the attenuating effect of vitamin E, as a natural antioxidant, against this toxicity. Thirty Albino rats were divided into 3 groups (n = 10): control group, AgNPs group receiving 2 mg/kg daily for 28 days, and AgNPs and vitamin E group receiving AgNPs the same as the previous group in addition to vitamin E at a dose of 100 mg/kg. Microscopic, ultrastructural, and cytokeratin immune-reactivity examination of the glands were performed. The AgNPs group showed noticeable degeneration in all structures of the gland as evident in the histological and ultrastructural examination. The AgNPs and vitamin E group revealed an improvement of the glandular elements. A significant increase in cytokeratin immune expression was found after comparison of both groups (p = 0.01). This current study shows that vitamin E has powerful antioxidant properties, which can combat the cytotoxic effect caused by AgNPs. Further studies are deemed necessary to confirm this finding using other immunohistochemical markers, such as myosin and E-cadherin.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Attenuating Effect of Vitamin E against Silver Nano Particles Toxicity in Submandibular Salivary Glands

et al. 2021

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“…Silver nanoparticles caused a significant toxicity in the salivary glands of albino rats. These toxic effects, however; can be effectively reversed by administration them with vitamin C [17]. Another study showed that vitamin C is a hepatoprotective agent against ZnO-NP toxicity [18].…”

Section: Introductionmentioning

confidence: 99%

Bioaccumulation and Evaluation of Magnesium Oxide Nanoparticles Toxicity and Combination Effects of Vitamin E and C with it on Exposed Male Rats

Majeed

Mohammed

Mohammad

2023

KJAR

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In the recent years, MgO nanoparticles (MgO NPs) have been one of the metal oxide nanoparticles that used in various medicinal fields. Despite MgO NPs' widespread use, a little is known regarding their cytotoxic effects on health. The purpose of this study was to determine the toxic dose of MgO NPs that prepared by the sol-gel method and the roles of vitamins E and C in the toxicity that is produced by MgO NPs. The MgO NPs were characterized and several amounts (62.5, 125, 250, and 500 mg/kg BW) were orally administrated for 28 days to male Wister rats. Toxic dosimetry was evaluated using metal content analysis, hematological, biochemical, histopathological, and serum ROS estimation. Then toxic doses of MgO NPs were administered orally with vitamins E and C, or a combination of them for six weeks, twice a week. The result showed 500 mg/kg bw/day MgO NPs is considered as a toxic and leads to a significant Mg bioaccumulation in the liver (P < 0.001) and significantly increased serum ROS (P<0.001) and histopathological damage in the liver and kidney with an immunologic response. Administration of vitamin E with MgO NPs significantly increased serum ROS and adversely affected blood parameters and histological damage to the liver and kidney. The combination of vitamins E and C with MgO NPs significantly reduced the immunologic response to MgO NPs, but serum ROS levels increased significantly with histopathological damage in vital organs. Vitamin C significantly reduced MgO NPs-induced damage in kidney and liver tissues. In the conclusion, MgO NPs are toxic at high doses. Administration of vitamin E alone or combined vitamin E and C with toxic doses of MgO NPs increases their toxicity. However, vitamin C was shown to be histopathologically protective. Therefore, a serious caution should be considered when vitamins are administered with nanoparticles.

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Histological Assessment of Systemic Toxicity Induced by Zinc oxide Nanoparticles and the Prophylactic Potency of Ascorbate in Albino Rats

Osman,

Afify,

Frag

et al. 2022

Journal of Microscopy and Ultrastructure

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Background: Nanoparticles of zinc oxide (ZnO-NPs) are frequently implemented in cosmetics, additives, and electronic devices. Moreover, their applications extend to water treatment, drug delivery, and cancer therapy. As a result, NP toxicity became an essential subject in biosafety research. Aim: Using histological and immunohistochemical analysis, we attempted to investigate whether ascorbate (“vitamin C”) (VC) could protect liver, lung, and spleen tissues from ZnO-NP systemic toxicity. Materials and Methods: Rats were classified as control group, NP group injected intraperitoneally (IP), once by dissolved ZnO-NPs (200 mg/kg), and NP + VC group injected IP, once by dissolved ZnO-NPs (200 mg/kg) and then ingested 100 mg/kg of VC orally. Blood samples were collected. Liver, lung, and spleen specimens were prepared for light, electron microscopic, and immunohistochemical analysis. Results: In comparison to the control group, the NP group’s liver enzyme, i.e. aspartate transaminase and alanine transaminase, values and counts of white blood cells (WBCs) were higher on the 7th day, but their red blood corpuscle (RBC) count, hemoglobin (Hgb) level, platelet count, and albumin values were lower. Histopathological analysis of liver, lung, and spleen tissues showed severe toxicity manifested by cell apoptosis, mononuclear cell infiltration, dilated blood vessels, and hemorrhage. In addition, the NP group showed a significantly higher expression of Ki67 and caspase-3 immunoreactivity. The biochemical, hematological, and histopathological results of the NP + VC group improved overall, reflecting VC’s protective effect against systemic toxicity. Conclusion: Our study revealed that ascorbate (VC) inhibited the systemic toxicity prompted by ZnO-NPs in lung, liver, and spleen tissues, indicating its importance for future treatment with ZnO-NPs.

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Histological and Ultrastructural Study Of Silver Nanoparticles Toxicity and The Possible Protective Effect Of Vitamin C On Submandibular Salivary Glands Of Albino Rats

Cited by 3 publications

References 0 publications

Attenuating Effect of Vitamin E against Silver Nano Particles Toxicity in Submandibular Salivary Glands

Attenuating Effect of Vitamin E against Silver Nano Particles Toxicity in Submandibular Salivary Glands

Bioaccumulation and Evaluation of Magnesium Oxide Nanoparticles Toxicity and Combination Effects of Vitamin E and C with it on Exposed Male Rats

Histological Assessment of Systemic Toxicity Induced by Zinc oxide Nanoparticles and the Prophylactic Potency of Ascorbate in Albino Rats

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