Histological changes in masticatory muscles of mdx mice

Spassov, Alexander; Gredes, Tomasz; Gedrange, Tomasz; Lucke, Silke; Pavlović, Dragan; Kunert‐Keil, Christiane

doi:10.1016/j.archoralbio.2010.02.005

Cited by 27 publications

(38 citation statements)

References 38 publications

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“…Unlike the other masticatory muscles, in the tongue we did not find changes in the level of cav-1. This could explain our earlier results which showed a virtually unaffected tongue muscle, with absence of inflammation and collagen accumulation [27].…”

Section: Discussionsupporting

confidence: 52%

“…masseter and temporal, were severely affected by dystrophin deficiency, while mdx tongue muscle was partially spared. These chang- www.fhc.viamedica.pl es were found to be accompanied by intensive regeneration, with numerous inflammatory foci and new vessel formations [27]. In our study, we analyzed the expression of caveolin-1 and -3 as well as VEGF in the masticatory muscles of the animal model of DMD, the mdx mice.…”

Section: Discussionmentioning

confidence: 96%

“…In the tested dystrophic masticatory muscles, we found an increase in the expression of cav-1. Because both protein and mRNA were extracted from the whole muscle sample containing muscle fibers, blood vessels and connective tissue, it seems possible that the increased concentration of cav-1 is due to: (i) formation of new blood vessels during regeneration: the numerous inflammatory foci found in mdx masticatory and soleus muscles are associated with new vessel formation and leukocyte accumulation [27]; (ii) by fibroblasts, which are responsible for increased collagen and connective tissue content in mdx muscles [27,39,40]; and (iii) by the physiological abundance of adipocytes. It is known that cav-1 is mainly expressed in adipocytes, smooth muscle cells, fibroblasts, and endothelial cells [5,41,42].…”

Section: Discussionmentioning

confidence: 99%

“…Recently, it has been shown that the masticatory muscles of mdx mice are characterized by typical cycles of necrosis and regeneration associated with inflammation, muscle fiber hypertrophy and collagen accumulation [27].…”

Section: Introduction Introduction Introduction Introduction Introducmentioning

confidence: 99%

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Caveolin-1, caveolin-3 and VEGF expression in the masticatory muscles of mdx mice

Kunert‐Keil¹,

Gredes²,

Lucke³

et al. 2011

Folia Histochem Cytobiol.

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Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

Section: Introduction Introduction Introduction Introduction Introducmentioning

confidence: 99%

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Caveolin-1, caveolin-3 and VEGF expression in the masticatory muscles of mdx mice

Kunert‐Keil¹,

Gredes²,

Lucke³

et al. 2011

Folia Histochem Cytobiol.

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“…Furthermore, mdx skeletal muscles often present varying patterns of histopathology [19]. In a recent preliminary study from our laboratory, we also confirmed such intermuscular differences for the masticatory muscles of 100-day old mdx mice [20]. Thus, mdx masseter and temporal muscles contained mostly regenerated fibres, some inflammatory foci and increased collagen content, whereas the mdx tongue muscles seemed almost to have been spared by dystrophinophinopathy.…”

Section: Introductionsupporting

confidence: 82%

The expression of myogenic regulatory factors and muscle growth factors in the masticatory muscles of dystrophin-deficient (MDX) mice

Spassov

Gredes

Gedrange

et al. 2011

Cellular and Molecular Biology Letters

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Abstract:The activities of myogenic regulatory factors (MRF) and muscle growth factors increase in muscle that is undergoing regeneration, and may correspond to some specific changes. Little is known about the role of MRFs in masticatory muscles in mdx mice (the model of Duchenne muscular dystrophy) and particularly about their mRNA expression during the process of muscle regeneration. Using Taqman RT-PCR, we examined the mRNA expression of the MRFs myogenin and MyoD1 (myogenic differentiation 1), and of the muscle growth factors myostatin, IGF1 (insulin-like growth factor) and MGF (mechanogrowth factor) in the masseter, temporal and tongue masticatory muscles of mdx mice (n = 6 to 10 per group). The myogenin mRNA expression in the mdx masseter and temporal muscle was found to have increased (P < 0.05), whereas the myostatin mRNA expressions in the mdx masseter (P < 0.005) and tongue (P < 0.05) were found to have diminished compared to those for the controls. The IGF and MGF mRNA amounts in the mdx mice remained unchanged. Inside the mdx animal group, gender-related differences in the mRNA expressions were also found. A higher mRNA expression of myogenin and MyoD1 in the mdx massterer and temporal muscles was found in females in comparison to males, and the level of myostatin was higher in the masseter and tongue muscle (P < 0.001 for all comparisons). Similar gender-related differences were also found within the control groups. This study reveals the intermuscular differences in the mRNA expression pattern of myogenin and myostatin in mdx mice. The existence of these differences implies that dystrophinopathy affects the skeletal muscles differentially. The finding of gender-related differences in the mRNA expression of the examined factors may indicate the importance of hormonal influences on muscle regeneration.

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