Histological changes in rabbits after application of medicaments and cosmetic bases

Rantuccio, F; Scardigno, A; Conte, A; Sinisi, D.; Coviello, C.

doi:10.1111/j.1600-0536.1979.tb04912.x

Cited by 13 publications

(14 citation statements)

References 4 publications

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“…Several studies of the histology of experimentally induced cumulative irritation have been reported (7–10). In these studies, medicaments and cosmetic bases showed different grades of irritancy, however, it was not clearly described whether differences exist according to the chemical nature of irritants.…”

Section: Discussionmentioning

confidence: 99%

Pathological findings in cumulative irritation induced by SLS and croton oil in hairless mice

Moon

Seo²,

Han³

et al. 2001

Contact Dermatitis

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It is known that the pathological features of acute irritant contact dermatitis are specific according to the irritant. However, in chronic irritant contact dermatitis, it is not clear whether specific patterns exist. To investigate whether the specific pathology of acute irritant contact dermatitis is sustained in chronic irritant contact dermatitis, sodium lauryl sulfate (SLS) and croton oil were applied 3x a week for 2 weeks on the dorsal skin of hairless mice using Finn Chambers. The pathologic changes induced by irritants at various concentrations were evaluated using H&E and Luna's staining, as well as immunohistochemistry for 5-bromo-2-deoxyuridine (BrdU), keratin 6 and loricrin. Our results showed that epidermal hyperplasia and inflammatory infiltration were relatively marked in the groups treated with higher concentrations of irritants. These features were more prominent in the 1% croton oil treated group than in the 0.25% SLS treated group. However, lower concentrations of irritants resulted in very similar histological changes, characterized by epidermal hyperplasia with minimal inflammatory infiltration, irrespective of the chemical. Our results suggest that the histological responses to irritants vary with concentration in cumulative irritation, as in acute irritation, but repetitive mild irritation may evoke common histological changes, characterized by epidermal hyperplasia with minimal inflammatory infiltration, irrespective of the chemical used.

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Section: Discussionmentioning

confidence: 99%

Pathological findings in cumulative irritation induced by SLS and croton oil in hairless mice

Moon

Seo²,

Han³

et al. 2001

Contact Dermatitis

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“…There is limited information on dermal irritation to petrolatum in laboratory animals. In addition to the two previous publications (Budhiraja et al ., ; Rantuccio et al ., ), acanthosis in the skin of guinea pigs has been described (Wollmann et al ., ). As indicated previously, dermal irritation (either clinically or microscopically) was not observed in Wistar‐Han rats, CD1 or C57Bl/6J mice or Göttingen minipigs treated topically with white petrolatum.…”

Section: Discussionmentioning

confidence: 95%

“…In the other earlier study (Budhiraja et al ., ), petrolatum (white and yellow) was applied for 10 days and skin for histological examination was obtained on Study Day 11 and Study Day 15. Similar to the findings observed in this study, erythema and edema was noted clinically, with thickening of the epidermis, hyperkeratosis and marked cellular infiltration of the dermis (Rantuccio et al ., ). Budhiraja et al .…”

Section: Discussionmentioning

confidence: 97%

“…The objective of this study was to describe dermal irritation observed in New Zealand white rabbits treated topically with white petrolatum. Although unexpected, a thorough review of the literature revealed similar findings had been reported in rabbits (Budhiraja et al ., ; Rantuccio et al ., ) and rats (Budhiraja et al ., ) more than three decades ago. In general, the macroscopic as well as the onset of clinical changes reported in the two previous studies are generally similar to that observed in our study although the treatment duration was slightly different.…”

Section: Discussionmentioning

confidence: 99%

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Dermal irritation of petrolatum in rabbits but not in mice, rats or minipigs

Chandra

Peterson

Melich

et al. 2013

J of Applied Toxicology

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Petrolatum is widely used in cosmetics, topical pharmaceuticals and also as a vehicle in dermal toxicity studies. New Zealand white rabbits treated with white petrolatum (vehicle control) in a 2-week dermal irritation study exhibited moderate to severe erythema starting on Day 7 that subsided towards the end of the study. Histological examination of abraded and non-abraded petrolatum-treated skin obtained at termination (Day 15) revealed mild acanthosis, hyperkeratosis, dermal edema with mixed inflammatory cells in the dermis. Macroscopic and microscopic features noted in rabbits were consistent with dermal irritation to petrolatum. Wistar-Han rats, CD1 mice, C57/Bl/6J mice and Göttingen minipigs treated topically with white petrolatum did not exhibit clinical or histologic evidence of dermal irritation. Therapeutic agents developed for topical application are generally tested in rabbits during some point in development. Interpretation of skin irritation data from a single species can impact risk assessment for humans and on product labeling.

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“…Treatment of the SC with cationic surfactants can markedly increase the transport of hydrophilic substances. 13,14) Application of even diluted solutions of cationic surfactants to the skin changes the structure of the lipid bilayers in the skin.…”

Section: Back Diffusion Of Glucose Enhanced By Surfactant Pretreatmentsmentioning

confidence: 99%

Noninvasive Glucose Monitoring by Back Diffusion via Skin: Chemical and Physical Enhancements

Huang

Fang

et al. 2003

Biological & Pharmaceutical Bulletin

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Blood glucose levels are routinely obtained by invasive and painful methods using glucose meters and test strips. The development of less invasive or non invasive techniques would be beneficial for diabetes patients. In this study, a noninvasive method was evaluated using the back diffusion of glucose across skin with or without permeation enhancement methods. An in vitro model was utilized. The stratum corneum (SC) was the predominant barrier for both back and forward diffusion of glucose across skin. Surfactants with various charges and essential oils (cyclic monoterpenes) were used as chemical enhancers to promote the back diffusion of glucose. A cationic surfactant (benzalkonium chloride) showed the highest enhancement, followed by anionic and nonionic surfactants. d-Limonene and 1,8-cineole dispersed in appropriate proportions of ethanol could enhance the glucose diffusion after pretreatment of the skin surface. Electroporation, defined as a physical method, significantly increased the amount of glucose that diffused back. The percentages of diffused glucose by 300 V (volts) and 500 V high voltage pulses on skin for 10 min were found to be 45 and 75 times greater than the control group, respectively.

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Histological changes in rabbits after application of medicaments and cosmetic bases

Cited by 13 publications

References 4 publications

Pathological findings in cumulative irritation induced by SLS and croton oil in hairless mice

Pathological findings in cumulative irritation induced by SLS and croton oil in hairless mice

Dermal irritation of petrolatum in rabbits but not in mice, rats or minipigs

Noninvasive Glucose Monitoring by Back Diffusion via Skin: Chemical and Physical Enhancements

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