Histological study, including ultrastructural quantification, of the periodontal ligament in the lathyritic rat mandibular dentition

Shore, R.C.; Berkovitz, Β.Κ.B.; Moxham, B.J.

doi:10.1016/0003-9969(84)90100-6

Cited by 13 publications

(2 citation statements)

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“…Shore, Berkovitz and Moxham (38) have suggested that care must be taken in choosing appropriate controls for use in lathyritie studies to distinguish the true effects of lathyrogens from those related to dietary deficiency. In an additional experiment, we eould not find a significant dilTerence in the meehanica!…”

Section: Aan (mentioning

confidence: 99%

Effects of lathyrogens on the mechanical strength of the periodontal ligament in the rat mandibular first molar

Ohshima

Nakamura

Chiba

1989

J of Periodontal Research

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Effects of lathyrogens such as aminoacetonitrile (AAN), beta-aminopropionitrile (BAPN) and cysteamine--known inhibitors of cross-linking of collagen--on the mechanical strength of the periodontal ligament of the rat mandibular first molar were examined by measuring the ultimate load required to extract the tooth from its socket in the dissected jaw. Single injections of AAN (40 approximately 100 mg/100 g body weight) or of BAPN (100 mg/100 g body weight) caused significant decreases of the mechanical strength 24 h after administration of the drugs but that of cysteamine (30 mg/100 g body weight) did not. Significant correlations between the dose of AAN or of BAPN and the mechanical strength were found following daily administrations of the drugs for 5 days. The relative potency of AAN to BAPN was estimated to be 4.5 by a slope ratio assay. The rapid appearance and disappearance of the effect of lathyrogens on the mechanical strength of the periodontal ligament of the rat mandibular first molar provide further evidence that the turnover of the collagen in the tissue is fast. The half-time of collagen synthesis was estimated to be approximately 3 d.

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Section: Aan (mentioning

confidence: 99%

Effects of lathyrogens on the mechanical strength of the periodontal ligament in the rat mandibular first molar

Ohshima

Nakamura

Chiba

1989

J of Periodontal Research

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“…1 Lathyrogens act on the enzyme lysyl oxidase and specifically inhibit the formation of cross-links between collagen molecules. 2 Lathyritic agents cause weakness and deformity in connective tissues, preventing normal maturation of collagen by inhibiting the formation of cross-links. 3 A lathyritic agent, b-aminoproprionitrile (b-APN), was isolated from the sweet pea, L. odouratus, by Selye in 1957 and is considered an appropriate agent for studying connective tissue metabolism.…”

Section: Introductionmentioning

confidence: 99%

The role of ascorbic acid on collagen structure and levels of serum interleukin-6 and tumour necrosis factor-alpha in experimental lathyrism

et al. 2004

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Lathyrism is characterized by defective collagen synthesis due to inhibition of lysyl oxidase, an enzyme essential for interfibrillar cross-linking. The lathyritic agent beta-aminoproprionitrile (beta-APN) is considered an appropriate agent for studying connective tissue metabolism. We investigated the effects of ascorbic acid on collagen structure and serum cytokine levels in experimentally induced lathyrism. Forty Wistar rats weighing 200-300 g were used in the study: three test groups of 10 rats each (groups 2, 3 and 4) and 10 rats used as a control group (group 1). Experimental lathyrism was induced with daily subcutaneous injections of beta-APN in the test groups for 40 days. On the 40th day, skin biopsies were taken from the control group (group 1) and group 2, to evaluate the effect of beta-APN on dermal collagen. After the 40th day, 10 rats received ascorbic acid 100 mg/kg intraperitoneally daily for 15 days (group 3) and 10 rats (group 4) received no medication and served as a control for group 3. On the 55th day, skin biopsies were taken from groups 3 and 4. Serum concentrations of interleukin-6 and tumour necrosis factor-alpha were assessed in each group by enzyme-linked immunosorbent assay. Ultrastructural examination of the skin biopsies in group 1 revealed normal-appearing epidermal and dermal structures. Group 2 showed disorganization of the epidermis and collagen structure, and vacuolization of the endoplasmic reticulum in fibroblasts. In group 3, ultrastructural examination revealed significant improvement in the structure of dermal collagen after administration of ascorbic acid, whereas the changes in group 4 were unremarkable. Ascorbic acid administration significantly decreased the concentrations of serum cytokines in group 3 compared with group 2 (P < 0.001). Ascorbic acid administration significantly improved dermal collagen structure and serum cytokine levels in experimental lathyrism.

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The mobility of the lathyritic rabbit mandibular incisor in response to axially-directed extrusive loads

Moxham

Berkovitz

1984

Archives of Oral Biology

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Histological study, including ultrastructural quantification, of the periodontal ligament in the lathyritic rat mandibular dentition

Cited by 13 publications

References 20 publications

Effects of lathyrogens on the mechanical strength of the periodontal ligament in the rat mandibular first molar

Effects of lathyrogens on the mechanical strength of the periodontal ligament in the rat mandibular first molar

The role of ascorbic acid on collagen structure and levels of serum interleukin-6 and tumour necrosis factor-alpha in experimental lathyrism

The mobility of the lathyritic rabbit mandibular incisor in response to axially-directed extrusive loads

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