Histology and immunohistochemistry of the developing juxta-oral organ in mice

Short histological kaleidoscope – recent findings in histology. Part III

“…The stratum nervosum consists of nerve fibers and sensory corpuscles (Pacinian, Ruffini’s, glomerular or lamellar corpuscles), being described as a mechanosensory structure. Also, in 2015, Kobayashi et al demonstrated the presence of a ring of sensory endings between the parenchyma and stratum fibrosum internum [ 187 ]. Suarez-Quintanilla and the research team discovered that the connective layers showed intense immunoreactivity for a blood vessel marker (CD34), and thus suggested the presence at this level of structures resembling capillaries [ 182 , 184 ].…”

The Juxtaoral Organ: From Anatomy to Clinical Relevance

“…The study by Ito and colleagues [ 18 ] also suggested that the differences observed are linked to the phylogenetic order and the functional requirements of different species and stressed of the need for further studies on comparative anatomy and embryogenesis in other species to support/contradict their hypotheses. Kobayashi et al [ 19 ] studied the development of the juxtaoral organ in mice and confirmed that its morphology is very different from that in humans. Notably, for the first time, the authors described the immunohistochemical and ultrastructural characteristics of the epithelial, mesenchymal and neuronal components of the juxtaoral organ in both developing embryos and mature mice.…”

“…Especially in the middle and posterior segments, the juxtaoral organ is outside the buccinator muscle, but remains located adjacent to the oral mucosal epithelium. Furthermore, Kobayashi and colleagues [ 19 ] observed in 8-week-old mice, that the parenchyma of the juxtaoral organ is positive for p63, suggesting that the cell cycle of the parenchymal cells in the juxtaoral organ of adult mice is stopped or proceeds very slowly, unlike the rapid turnover of the oral epithelial cells. The parenchyma of the juxtaoral organ in adult mice also showed epithelial cells positive for cytokeratin 14, a typical marker of the basal cells of the stratified epithelium.…”