Histology and tetracycline labeling of a single section of alveolar bone of first molars in the rat

Abstract

We observed the histology and tetracycline (TC) labeling in a single frontal section of alveolar bone of upper first molars of adolescent rats. A single injection of TC was administered intraperitonealy in adolescent rats. After three weeks, the upper jaws were immersed rapidly in liquid nitrogen and sectioned. Five micrometer unfixed, undecalcified frozen sections were cut and observed by light and fluorescence microscopy. Frontal sections of the upper first molar area revealed that the structural relationships among the roots, the periodontal ligament and the alveolar bone, and also between the cervical enamel and the attachment epithelium were well preserved. The TC labeling lines in the sections were very clear and distinguished new bone from old bone. The brightness of the lines differed among regions. An analysis of the brightness in the same section suggested a difference in the bone forming activity at the time of injection.

Calcification of degenerating tissues in the periodontal ligament during tooth movement

“…With regard to the fine calcified particles, there is a possibility that they were fine fragments of either bone, cementum or dentin, attached to the sections during the sectioning process. Therefore, careful examination of unfixed undecalcified sections of PDL was performed and this confirmed that there was no sign of the attachment as in previous studies (12, 13).…”

“…compound was completely frozen. The frozen blocks were sectioned horizontally and frontally using a cryostat (Reitz, Wetzlar, Germany) with a super‐hard tungsten steel knife (Meiwa Shoji Ltd, Tokyo, Japan) (11), and 5‐µm undecalcified frozen serial cross‐ and frontal‐sections were collected and freeze‐dried for 1 h in the cryostat (11, 12).…”

Phosphatidylinositol-dependent bond between alkaline phosphatase and collagen fibers in the periodontal ligament of rat molars

“…Whole periodontal tissues, gingiva, periodontal ligament, alveolar bone, and cementum were observed in the sections. Structural relationships among the tissues were very well preserved (Nakamura et al 2000).…”

“…compound, and sectioned using a super-hard tungsten steel knife (Meiwa Shoji, Tokyo, Japan) (Nakamura et al 1994). Serial frontal sections, 5 mm thick (Nakamura et al 2000), were collected individually with powerful adhesive tapes (Kawamoto and Shimizu 1986) and were then freeze-dried for 1 h in the cryostat.…”

Tooth displacement and root dilaceration after trauma to primary predecessor: an evaluation by computed tomography

“…During odontogenesis, any factor that changes the metabolic and physical conditions around the tooth germ may disturb its development. Among metabolic alterations, the ability of tetracycline (1) or fluorides (2) to stain dentine and enamel have been extensively discussed. Traumatic injuries to deciduous dentition are the most important physical factors that can disturb permanent tooth formation (3, 4).…”