Histology of human teeth: Standard and specific staining methods revisited

Widbiller, Matthias; Rothmaier, C.; Saliter, D.; Wölflick, M.; Rosendahl, Andreas; Buchalla, Wolfgang; Schmalz, Gottfried; Spruß, Thilo; Galler, Kerstin M.

doi:10.1016/j.archoralbio.2021.105136

Cited by 21 publications

(14 citation statements)

References 55 publications

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“…The incisor extracted from sheep in this study had similar histological features in comparison with human teeth (Widbiller et al, 2021). The layer of odontoblastic cells was also observed with heterogeneous morphology.…”

Section: Discussionsupporting

confidence: 56%

Volumetry of ovine incisors dental pulp for further regenerative therapy

Ems,

Severino,

Nisolle

et al. 2023

Anat Histol Embryol

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Mesenchymal stem cells (MSCs) are used for regenerative therapy. Dental pulp MSCs make extracted wisdom teeth a useful resource in humans. Preclinical validation of regenerative therapies requires large animal models such as the sheep. Since stem cells can be retrieved from the dental pulp of ovine incisors, the best age to extract a maximal volume of dental pulp needs to be defined. The objective of this ex vivo study was to quantify incisors dental pulp volume, in sheep of various age. Three jaws were dedicated to histology (one per age group); the others were imaged with a computed tomography scanner [3 years‐old (n = 9), 4 (n = 3) and 6 (n = 5)]. The incisors dental pulp volume was measured after 3D reconstruction. Multiple linear regression showed that dental pulp volume of ovine incisors decreases with age (β‐estimate = −3.3; p < 0.0001) and teeth position from the more central to the more lateral (β‐estimate = −4.9; p = 0.0009). Weight was not a relevant variable in the regression model. The dental pulp volume ranged from 36.7 to 19.6 mm3 in 3‐year‐old sheep, from 23.6 to 11.3 in 4‐year‐old sheep, and from 19.4 to 11.5 in 6‐year‐old sheep. The pulp volume of the most central teeth (first intermediate) was significantly higher than the most lateral teeth (corner). Haematoxylin–Eosin–Safran of the whole incisors, and of isolated dental pulps demonstrated a similar morphology to that in humans. The first intermediate incisor of 3‐year‐old sheep should be selected preferentially in preclinical research to retrieve the highest volume of dental pulp.

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Section: Discussionsupporting

confidence: 56%

Volumetry of ovine incisors dental pulp for further regenerative therapy

Ems,

Severino,

Nisolle

et al. 2023

Anat Histol Embryol

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“…To assess the effectiveness of the isolation procedure, amniotic tissue before digestion as well as after the first and second digestion step was fixed in formalin for 2 h and processed for histology. Histological processing and HE staining was performed according to a previously published protocol [ 85 ]. Furthermore, flow cytometry was conducted to evaluate the antigen profile of the isolated cells.…”

Section: Methodsmentioning

confidence: 99%

Human Amnion Epithelial Cells: A Potential Cell Source for Pulp Regeneration?

Bucchi

Ohlsson

Anta

et al. 2022

IJMS

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The aim of this study was to analyze the suitability of pluripotent stem cells derived from the amnion (hAECs) as a potential cell source for revitalization in vitro. hAECs were isolated from human placentas, and dental pulp stem cells (hDPSCs) and dentin matrix proteins (eDMPs) were obtained from human teeth. Both hAECs and hDPSCs were cultured with 10% FBS, eDMPs and an osteogenic differentiation medium (StemPro). Viability was assessed by MTT and cell adherence to dentin was evaluated by scanning electron microscopy. Furthermore, the expression of mineralization-, odontogenic differentiation- and epithelial–mesenchymal transition-associated genes was analyzed by quantitative real-time PCR, and mineralization was evaluated through Alizarin Red staining. The viability of hAECs was significantly lower compared with hDPSCs in all groups and at all time points. Both hAECs and hDPSCs adhered to dentin and were homogeneously distributed. The regulation of odontoblast differentiation- and mineralization-associated genes showed the lack of transition of hAECs into an odontoblastic phenotype; however, genes associated with epithelial–mesenchymal transition were significantly upregulated in hAECs. hAECs showed small amounts of calcium deposition after osteogenic differentiation with StemPro. Pluripotent hAECs adhere on dentin and possess the capacity to mineralize. However, they presented an unfavorable proliferation behavior and failed to undergo odontoblastic transition.

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“…Lung specimens were fixed in 10% buffered formalin (pH 7.4) fixative solution for 24 hours, dehydrated in ascending grades of ethanol, cleared in xylol, and embedded in paraffin. Tissues were processed for preparation of paraffin blocks to get paraffin sections (5 μm in thickness), which were stained by conventional H&E to assess the cell nucleus, cytoplasm and organelles [ 21 ], Masson’s Trichrome that allows the differentiation of collagen fibers [ 22 ] and periodic acid shift (PAS stain) to assess glycogen presence which reflects the state of energy stores [ 23 , 24 ].…”

Section: Histological Study Of Lungmentioning

confidence: 99%

Quercetin ameliorates acute lung injury in a rat model of hepatopulmonary syndrome

Nassef

Hamid

Abusikkien

et al. 2022

BMC Complement Med Ther

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Background Common bile duct ligation (BDL) is a rat experimental model to induce biliary cirrhosis. Lung fibrosis and pulmonary vascular angiogenesis and congestion are the most common complications of biliary cirrhosis that is known as hepatopulmonary syndrome. The aim of the present work is to investigate the acute lung injury in a BDL model and to investigate the possible protective effect of quercetin on this injury. Methods Twenty-four adult male albino rats of the Wister strain (weighing 150–250 g). Animals were divided into 3 groups, with 8 rats each: Group I: Sham-operated group (control). Group II: Bile duct ligation group (BDL) sacrificed after 28 days from the surgery. Group III: Quercetin-treated bile duct ligation group (Q-BDL) was given orally by gastric gavage in a dose of 50 mg/kg/day, starting from the 4th day of the operation until the 28th day. At the end of the experiment, at day 28, all rats were sacrificed. Lung specimens were processed to measure Endothelin B receptor gene expression by PCR, lung surfactant by ELISA, “eNO” s by immunohistochemistry. Histological assessment was done using; H&E, Masson’s trichrome, PAS, toluidine blue-stained semi-thin sections, transmission electron microscope. Histomorphometric and statistical studies were done. Results BDL group showed significant increase in lung index together with mononuclear cellular infiltration denoting lung inflammatory state. Also, the significant increase in pulmonary endothelial nitric oxide synthase ("eNO" s) area percent and endothelin B receptor (ETB) gene expression indicates enhanced angiogenesis. Pulmonary surfactant concentration was significantly decreased together with thickening of interalveolar septa denoting lung injury and fibrosis. Quercetin led to significant decrease in lung index, pulmonary "eNO" s area percent, ETB gene expression and significant increase in pulmonary surfactant concentration. Quercetin treatment improved histological changes and morphometric measurements, limited mononuclear cellular infiltration and decreased perivascular and perialveolar collagen deposition. Conclusion Quercetin ameliorates the hepatopulmonary syndrome-induced lung injury through its anti-inflammatory, antioxidative and antifibrotic effects.

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Histology of human teeth: Standard and specific staining methods revisited

Cited by 21 publications

References 55 publications

Volumetry of ovine incisors dental pulp for further regenerative therapy

Volumetry of ovine incisors dental pulp for further regenerative therapy

Human Amnion Epithelial Cells: A Potential Cell Source for Pulp Regeneration?

Quercetin ameliorates acute lung injury in a rat model of hepatopulmonary syndrome

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