Histomorphological Evaluation of Wound Healing of Rabbit Oviduct after Microsurgical Reanastomosis with the Use of Autologous Fibrin Adhesive, Human Fibrin Adhesive or Poly-Glycolic Acid Suture

Abstract

The morphology of the healing process of microsurgical re-anastomosis of the rabbit oviduct with the use of fibrin adhesive, autologous and heterologous, and conventional sutures is described. Both oviducts in 48 rabbits were cut and reanastomoses were performed. The rabbits were killed at different intervals after the operations, ranging from 2 h to 28 days, and the anastomoses were evaluated by histomorphological examination.The autologous fibrin adhesive was absorbed after a week and an uncomplicated healing was observed. Heterologous fibrin adhesive caused a granulomatous inflammation interpreted as an immune reaction of the host to the foreign protein, and conventional suturing resulted in severe tissue damage with an intensive inflammatory reaction.

Important Factors Influencing the Strength of Autologous Fibrin Glue; the Fibrin Concentration and Reaction Time – Comparison of Strength with Commercial Fibrin Glue

“…This implies that the adhe sion of the sealant for the tissue is the decisive factor for gluing tissue [10]. Autologous fibrin glue is completely resorbed after several days by fibrinolysis and induces a minimum of tis sue reactions [9].…”

“…Several methods of preparing fibrin glue from the patients' own plasma or from single donor plasma have been described. The methods are based on the separation of fibrinogen by cryoprecipitation, by precipitation with ammoni um sulfate, polyethylene glycol, ethanol, or the use of a whole plasma fibrin gel [1,[5][6][7][8][9].…”

Effectiveness of Autologous Fibrin Sealant in Experimental Lung Surgery

“…This has not been the case in studies on rabbits where differences were found after sealing of their oviducts with hu man or autologous rabbit glue. The human glue caused a granulomatous inflammation which was interpreted as an immune reaction to the foreign protein, whereas the autologous glue resulted in uncomplicated healing [11], In summary, we found that air leak after experimental lung surgery was reduced signif icantly by the use of fibrin glue made from plasma by ethanol precipitation. The glue ap peared to be biocompatible and to function as a scaffold for cell growth.…”

Sutureless and Reduced Suture Anastomosis of Hollow Vessels With Fibrin Glue: A Review

“…Re ecting the 1943 ndings of Tarlov et al [38,39] regarding autologus plasma clots, more recent studies suggest that AFG obviates possible immunologic responses to non-AFG and consequently acclerates healing [31,46,[81][82][83]. Many methods of autologous brinogen have been described with varied bonding strengths [21,59,74,75,78,84].…”

“…Many methods of autologous brinogen have been described with varied bonding strengths [21,59,74,75,78,84]. Although AFG adhesive strengths are generally inferior to those of commercial products, they are suf cent for many of the attempted surgerical procedures [21,24,59,[83][84][85][86]. Most hematology laboratories can prepare AFG quickly and economically on the same day of surgery and even intraoperatively [79,84].…”