Histomorphology of Cervical and Uterine Epithelia of Crab-Eating Macaque, &lt;i&gt;Macaca fasciculari&lt;/i&gt;&lt;i&gt;s&lt;/i&gt;

Dávid, Gyula; Hafez, E. S. E.; Kamash, M.A.

doi:10.1159/000155665

Cited by 3 publications

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“…In this study, two PAS-positive cells, vacuolated and granular, were isolated from estrous, 5-d pseudopregnant and ovariectomized animals . Similar cell types have been described by others in the intact cervices of rabbits, cattle, and monkeys (7,15) . Vacuolated cells have also been observed in the postovulatory and pregnant rabbit (23) secretory granule-to-granule fusion and mucus release (29) .…”

Section: Discussionsupporting

confidence: 84%

Isolation and characterization of rabbit endocervical cells

Chilton¹,

Sv²,

Jm³

et al. 1980

The Journal of Cell Biology

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Three cytologically distinct cell populations were identified, in addition to ciliated cells, when a unit gravity sedimentation procedure was applied to pronase-dispersed rabbit endocervical cells. Two of these cell populations contained histochemically distinguishable (periodic acid-Schiff [PAS]) mucoproteins and were designated vacuolated and granular PASpositive cells. The third, designated as vacuolated PAS-negative, did not contain secretory granules . Cell integrity was confirmed by trypan blue dye exclusion, [3H]leucine incorporation, and ultrastructural analysis . To demonstrate hormonal modulation of endocervical cell morphology, cell distribution profiles were compared from animals in different hormonal states . In the absence of estrogen dominance, PAS-positive cells from 5-d pseudopregnant rabbits were reduced 50%, while vacuolated PAS-negative cells increased fourfold as compared with estrous cell populations. The PAS-positive cells sedimented toward the top of the gradient where the bovine serum albumin concentrations were lower, consistent with a reduction in the number of secretory granules . In the sustained absence of ovarian steroid hormones, the number of PAS-positive mucous cells from ovariectomized rabbits was reduced to only 4% of the total endocervical cell population .The biosynthetic capacity of isolated endocervical cells was determined by incubating the three nonciliated cell populations from estrous and 5-d pseudopregnant rabbits for 36 h with the mucin precursor, [14 C]N-acetyl-D-glucosamine . Only PAS-positive cells incorporated significant amounts of the radiolabel . Specific biosynthetic and secretory activities were the same for vacuolated and granular PAS-positive cells from both estrous and pseudopregnant rabbits. Cells isolated from ovariectomized rabbits did not incorporate significant amounts of labeled precursor. This study indicates that steroid hormones influence cervical secretions by modulating the type of endocervical cells.

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Section: Discussionsupporting

confidence: 84%

Isolation and characterization of rabbit endocervical cells

Chilton¹,

Sv²,

Jm³

et al. 1980

The Journal of Cell Biology

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A gross anatomical and histo-morphological study of the vagina of the emu (Dromaius novaehollandiae) and ostrich (Struthio camelus)

Madekurozwa¹

2008

Aust. J. Exp. Agric.

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The present study details anatomical features of the lining of the emu and ostrich vagina, which in birds may impede the forward movement of spermatozoa. Vaginal samples were collected for light and electron microscopy. Samples for light microscopy were fixed in Bouin’s fluid, while samples for electron microscopy were fixed in 3% glutaraldehyde. After fixation the samples were processed routinely. A series of broad annular mucosal folds bearing convoluted primary folds were present in both ratites. The lining of the vaginal folds was a combination of ciliated, non-ciliated, vesicular and mitochondrial cells. The non-ciliated and a few ciliated cells, lining the crypts, contained mucin droplets. The role of the mucus is unclear. The results of the study show a similarity in the gross anatomical and scanning electron microscope features of the vagina in the emu and ostrich. Differences in the cellular composition of the vaginal epithelium were observed at the transmission electron microscope level.

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Morphologic and Immunohistochemical Features of the Cynomolgus Macaque Cervix

Wood

2008

Toxicol Pathol

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Female macaques serve as an important model for the study of reproductive diseases in women. Here we summarize characteristics of the macaque cervix, with a particular emphasis on the cynomolgus macaque. Key anatomic features include a stratified squamous exocervix, squamocolumnar junction and transformation zone, and glandular endocervix with prominent colliculi. Endocervical eversion occurs with onset of ovarian cycling and regresses to varying degrees based on age, hormonal status, and individual conformation. The cervical epithelium in macaques is highly responsive to estrogens, which induce marked squamous epithelial maturation and glandular hypertrophy. Progestogen effects include further induction of endocervical secretory activity and release of viscous mucus, which functions in both mucosal immunity and fertility regulation. On immunohistochemistry, the squamous cervix shows strong expression of estrogen receptor α, the proliferation marker Ki67/MIB1, and cytokeratin 14, whereas endocervical epithelium strongly expresses estrogen and progesterone receptors and cytokeratins 18 and 19. These patterns of expression are altered in cervical intraepithelial neoplasia, which is a common spontaneous lesion of the macaque cervix associated with specific types of oncogenic papillomaviruses. This report highlights important similarities with human cervix that should be useful for future studies of genital infection, neoplasia, and immunity in the macaque model.Competing Interests: This article was sponsored by Covance Inc. and Schering-Plough. The author did not declare any other competing interests.

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Histomorphology of Cervical and Uterine Epithelia of Crab-Eating Macaque, <i>Macaca fasciculari</i><i>s</i>

Cited by 3 publications

References 1 publication

Isolation and characterization of rabbit endocervical cells

Isolation and characterization of rabbit endocervical cells

A gross anatomical and histo-morphological study of the vagina of the emu (Dromaius novaehollandiae) and ostrich (Struthio camelus)

Morphologic and Immunohistochemical Features of the Cynomolgus Macaque Cervix

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